Navegando por Autor "SANTOS, Ian Barroso dos"

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Análise da expressão de miRNAs em carcinoma hepatocelular
(Universidade Federal do Pará, 2014-02-11) SANTOS, Ian Barroso dos; DEMACHKI, Samia; SANTOS, Ney Pereira Carneiro dos; http://lattes.cnpq.br/7568391537270652; http://lattes.cnpq.br/1290427033107137
Hepatocellular carcinoma represents the most common primary malignancy of the liver and the fifth most common solid tumor worldwide. Highly lethal, remais a serious public health problem because of difficulties in early diagnosis and the development of effective therapeutic measures. Recent in the field of molecular biology studies suggest that define the profile of miRNAs in hepatocellular carcinoma may considerably influence the identification of risk factors associated with oncogenes and suppressor genes. The objective is to evaluate the expression of miRNA 135b, miRNA 181a-5p and miRNA 181a-3p in samples of Hepatocellular Carcinoma and Chronic Hepatitis C and correlate them so likely to seek biomarkers related to the mechanism of carcinogenesis. The research was done in six patients with hepatocellular carcinoma and twenty four cases of Chronic Hepatitis C, Para, northen Brazil. All samples Hepatocellular carcinoma underwent microdissection for subsequent RNA extraction. For the extraction of total RNA and microRNA All=Prep the DNA / RNA FFPE kit (quiagem), quantified by the Qubit® 2.0 Fluorometer (Invitrogen) for final concentration of 5ng/μL standard equipment was used. The cDNA was obtained using TaqMan® MicroRNA Reverse Transcription (Applied Biosystems). Statistical analyzes were performed in softwares SPSS 17.0, using the Mann-Whitney test, with significat differences in the expression levels of the miR181a-3p and miR 181a-5p in hepatocellular carcinoma (average 3.94 and 17.9, respectively) compared with chronic hepatitis C (average 1.18 to 1.8, respectively) with P-value of 0.005 and 0.003. In this study, it was observed that miRNAs 181a-3p and 181a-5p, especially the 181a-5p way were significantly more highly expressed in hepatocellular carcinoma samples when compared to non-tumor liver tissue with chronic hepatitis C. Therefore, microRNAs have interesting characteristics that favor them as possible in biological screening for early diagnosis of tumors and targeted therapies selected markers.