Navegando por Autor "SANTOS, Kemper Nunes dos"
Agora exibindo 1 - 3 de 3
- Resultados por página
- Opções de Ordenação
Item Acesso aberto (Open Access) Análise de polimorfismo na região promotora do gene da Interleucina 18 (-137 G/C e -607 C/A) em pacientes portadores do vírus da hepatite C de Belém, Pará(Universidade Federal do Pará, 2012-03-23) SANTOS, Kemper Nunes dos; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769Since its discovery in 1989, the hepatitis C virus (HCV) has been recognized as a major cause of chronic liver disease worldwide. Considered a public health problem worldwide involving between 170 to 350 million people infected. Host genetic factors have been implicated in the persistence of HCV infection. Studies suggest that two single nucleotide polymorphisms at position -607 C/A (rs1946518) and -137 G/C (rs187238) in the region of the gene IL-18 and have been found associated with the transcriptional promoter activity of IL -18, and potentially of IFN-γ, being associated with delayed viral clearance and persistence of the disease. We conducted a cross-sectional analytical study of the city of Belém-PA in 152 blood samples from patients infected with HCV and 188 uninfected controls. The samples were subjected to RT-PCR (Reverse Transcriptase - PCR) for detection of viral RNA and, subsequently, the PCR-RFLP (Restriction Fragment Length Polymorphism) to evaluate the polymorphism in the promoter region of IL-18 gene at positions -137 G/C and -607 C/A. The results showed no significant difference for IL-18 polymorphisms between patients and control group. But showed a significant difference for homozygous genotypes G/G (39.1%) at position -137 (OR = 3.00, CI [95%] = 1.24 – 7.22, p = 0.02), and A/A (21.7 %), position -607 (OR = 3.62, CI [95%] = 1.25 – 10.45, p = 0.03) among women than men (22.6% and 7.6%). The results showed evidence that among women, the presence of the polymorphism homozygous A/A (-607) acts as a protective factor against HCV infection, genotype as the A/A (-607) have been linked in some studies with liver disease and mild viral clearance.Item Acesso aberto (Open Access) Analysis of polymorphisms in the interleukin 18 gene promotor (-137 g/c and -607 c/a) in patients infected with hepatitis c virus from the brazilian amazon(Universidade Federal do Pará, 2015-09) SANTOS, Kemper Nunes dos; ALMEIDA, Marcella Kelly Costa de; FECURY, Amanda Alves; COSTA, Carlos Araújo da; MARTINS, Luisa CaricioBackground - The hepatitis C virus has been recognized as the leading cause of chronic liver disease in the world. Host genetic factors have been implicated in the persistence of hepatitis C virus infection. Single nucleotide polymorphisms at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the IL-18 gene promoter have been suggested to be associated with delayed hepatitis C virus clearance and persistence of the disease. Objective - Identify these polymorphisms in a population infected with hepatitis C virus from the Brazilian Amazon region. Methods - In a cross-sectional analytical study conducted in Belém, Pará, Brazil, 304 patients infected with hepatitis C virus were divided into two groups: group A, patients with persistent infection; group B, patients with spontaneous clearance. The control group consisted of 376 volunteers not infected with hepatitis C virus. Samples were analyzed by RT-PCR for the detection of viral RNA and by RFLP-PCR to evaluate the presence of the -137 G/C and -607 C/A IL-18 gene promoter polymorphisms. Results - Comparison of polymorphism allele frequencies between the patient and control groups showed a higher frequency of allele C at position -607 among patients (P=0.02). When the association between the polymorphisms and viral infection was analyzed, patients carrying genotype C/A at position -607 were found to be at higher risk of persistent hepatitis C virus infection (P=0.03). Conclusion - the present results suggest a possible role of the -607 IL-18 gene promoter polymorphism in the pathogenesis of hepatitis C virus infection.Item Acesso aberto (Open Access) Hepatitis B and C virus infection among Brazilian Amazon riparians(2011-10) OLIVEIRA, Claudia Suellen Ferro de; SILVA, Adenielson Vilar e; SANTOS, Kemper Nunes dos; FECURY, Amanda Alves; ALMEIDA, Marcella Kelly Costa de; FERNANDES, Adriana Prado; COSTA, Carlos Araújo da; FREITAS, Andrei Silva; CORVELO, Tereza Cristina de Oliveira; MARTINS, Luisa CaricioINTRODUCTION: Viral hepatitis is a major public health concern in Brazil. There are few past studies on this issue, especially among riparian communities. This study aims at determining the seroprevalence of viral hepatitis B and C in the riparian community of Pacuí Island, within the Cametá municipality of Pará State, Brazil. Moreover, this study aims to investigate the principal risk factors that this community is exposed to. METHODS: The current study has accessed blood samples from 181 volunteers who have answered an epidemiological questionnaire. Analyses on serological markers have been tested with commercial ELISA kits for detecting HBsAg, total anti-HBc, anti-HBs, and anti-HCV. Within seroreactive patients for HCV, RT-PCR and line probe assay have been performed to identify the viral genotype. RESULTS: In the serological marker analysis for hepatitis B, no reactivity for HBsAg, rate of 1.1% for total anti-HBc, and rate of 19.3% for anti-HBs have been observed. On hepatitis C, 8.8% seroprevalence has been found, in which 62.5% have gotten viral RNA. Among the risk factors studied, the following have been highlighted: non-use of condoms, sharing of cutting instruments, use of illicit drugs, and reports of family disease with HBV or HCV. CONCLUSIONS: The vaccination coverage against HBV is low, and the high prevalence of HCV within this community has been observed.