Navegando por Autor "SILVA, Michele Bahia do Vale"

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Acesso aberto (Open Access)
Diagnóstico molecular e frequência de anticorpos anti-Leishmania infantum chagasi em cães do município de Belém, Pará
(2014-03) SCHWANKE, Katiane; SILVA, Aryane Maximina Melo da; OLIVEIRA, Adlilton Pacheco de; SILVA, Michele Bahia do Vale; SILVEIRA, Fernando Tobias; SCOFIELD, Alessandra; CAVALCANTE, Gustavo Góes
Visceral leishmaniasis is a disease whose etiological agent in Brazil is Leishmania infantum chagasi. Dogs are considered urban reservoirs of the disease, being an indicator of the human cases occurrence. The present study aimed to diagnose L. infantum chagasi infection in stray and owned dogs in Belém, Pará State, by polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA) using two different antigens. Venous blood samples from adult dogs, regardless of gender or breed, of different neighborhoods in Belém-PA, were collected in tubes with and without anticoagulant to obtain DNA and serum, respectively. These animals were divided into two groups: stray dogs captured by the Center for Zoonosis Control (Group A) and owned dogs (Group B). Sera were analyzed by IFA testing for IgG using two different antigens: 1) Bio-Manguinhos/Fiocruz antigen kit (Ag-PRO) containing promastigotes of Leishmania sp. (Complex Major-Like), 2) Instituto Evandro Chagas Antigen (Ag-AMA) consisting of amastigotes of L. infantum chagasi. The evaluation of the two antigens was performed considering positive the reactions above the 1:80 dilution. Already PCR was performed with DNA isolated from whole blood of animals and amplified with the primers RV1 and RV2. Of the 335 samples analyzed, 10.4% (35/335) were positive by IFA (Ag-PRO) and 0.9% (3/335) with the Ag-AMA. The distribution of positive samples is given as follows: Group A 14.8% (25/169) with Ag-PRO and 1.2% (2/169) with Ag-AMA; Group B 6% (10/166) with Ag-PRO and 0.6% (1/166) with Ag-AMA, being that all samples positive by IFA with Ag-AMA also reacted with Ag-PRO, and none of the samples detected DNA of L. infantum chagasi. The findings of this study indicate that Belém can still be considered non-endemic area for canine visceral leishmaniasis and the nature of the antigen influences the result of the IFA for the detection of anti-L. infantum chagasi antibodies in dogs, and the IFA using promastigotes of Leishmania major-like antigen should be used with caution as a confirmatory diagnostic on epidemiological studies in non-endemic areas.
Acesso aberto (Open Access)
Occurrence of eggs and oocysts of gastrointestinal parasites in passerine birds kept in captivity in Para State, Brazil
(Universidade Federal do Pará, 2016-12) MATOS, Paulo Cesar Magalhães; SILVA, Michele Bahia do Vale; SOUZA, Paulo Geovani Silva; LIMA, Danillo Henrique da Silva; MORAIS, Rodrigo de; FARIAS, Diana Maria de; ALVARENGA, Laís Cristina Oliveira; GABRIEL, Áurea Martins; OLIVEIRA, Wanessa Batista Lima; CAVALCANTE, Gustavo Góes; AMARAL, Alessandra Scofield
he objective of this study was to detect helminth eggs and protozoan oocysts in samples of feces from birds of the order Passeriformes in Para State, Brazil. Fecal samples were collected individually from 403 passerine birds seized and kept in captivity in Para State. Samples were processed by the double centrifugation technique in saturated sucrose solution and the coccidial oocyst-positive samples were submitted to sporulation in potassium dichromate 2.0%. Helminth eggs and/or protozoan oocysts were observed in 43.18% (174/403) of the fecal samples examined. Coccidial oocysts were detected in 93.68% (163/174) of the positive samples, whereas helminth eggs were observed in 10.34% (18/174) of the positive samples. Oocyst sporulation occurred in 43.56% (71/163) of the samples, and only Isospora spp. oocysts were detected. Nematode eggs of the superfamilies Trichostrongyloidea (4.60%; 8/174), Ascaridoidea (0.57%; 1/174), and Trichuroidea (0.57%; 1/174) were diagnosed in the positive samples. Cestoda eggs were diagnosed in 2.87% (5/174), whereas Trematoda eggs were detected in 2.30% (4/174) of positive samples. Passerine birds seized and kept in captivity in the visited local presented parasitism by intestinal helminths and protozoan, with a predominance of infection with coccidia of the gender Isospora.