Navegando por Autor "SILVEIRA, Edvaldo Lima"

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Acesso aberto (Open Access)
Copaiba oil effect under different pathways in mice subjected to sepsis
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-08) BOTELHO, Nara Macedo; SILVEIRA, Edvaldo Lima; LOPES, Letícia Nobre; SANTOS, Felipe Augusto Folha; TEIXEIRA, Renan Kleber Costa; SILVA, Thaís Travassos da
PURPOSE: To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture. METHODS: Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals; Control group: submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed. The Kaplan-Meier curves of surviving time were realized. RESULTS: All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05). CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage.
Acesso aberto (Open Access)
Implicações do perfil citocínico TH22 nas formas polares da hanseníase
(Universidade Federal do Pará, 2016-08-29) SILVEIRA, Edvaldo Lima; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
BACKGROUND: Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae. Among the immunopathological aspects of leprosy is known that the defense is done by the cellular immune response, able to phagocyte and destroy the bacilli, mediated by cytokines and mediators from oxidation. The long-standing concept of a Th1-Th2 dichotomy in leprosy, with predominant Th1 in tuberculoid lesions and Th2 predominant in virchowian pacients, has recently been challenged. Furthermore, the Th22 response was identified as modulating Th1-Th2 in inflammatory skin diseases, but their roles in leprosy have not yet been elucidated. OBJECTIVE: Evaluate the tissue expression of cytokines involved in Th22 response in the polar forms of leprosy. METHOD: Patients with dermato-immunological diagnosis of leprosy were included and selected 31 patients, 16 with the tuberculoid (TT) form and 15 with lepromatous (LL). Immunohistochemistry for tissue immunostaining with antibodies against IL-13, IL-22, TNF-α and FGF-b was based on the method involving the formation of biotin-streptavidin peroxidase complex. Quantitation of the immunostaining was taken randomly from 05 fields viewed at 400x magnification microscope. In univariate analysis, frequencies, measures of central tendency and dispersion were obtained and for investigation of the hypothesis were applied the Mann-Whitney test and the Pearson correlation, considering a significance level of 5% (p ≤ 0.05). RESULTS: Regarding the immunostaining for IL-22 can be observed statistical difference among the groups and in the LL pole the average was 241.3 ± 44.63 cells/mm2, while in the TT form the mean was 90.39 ± 30.18 cells/mm2 (p <0.0001). Engaging the presence of IL-13, LL pole average occurrence was 85.76 ± 19.99 cells/mm2. In the TT pole the mean was 57.20 ± 14.73 cells/mm2 (p = 0.0002). Regarding the immunostaining for FGF-b, in the LL lesions, the mean incidence was 228.9 ± 45.13 cells/mm2, while in the TT form the mean was 47.80 ± 14.29 cells/mm2 (p <0,0001). For TNF-α, quantitative analysis was statistically significant in TT form where the average of the cells expressing the cytokine was 99.74 ± 30.14 cells/mm2 when compared to the LL form where the results were 62.08 ± 13.67 cells/ mm2 (p = 0.0008). CONCLUSION: The Th-22 response, mediated by IL-22, has fundamental importance in the pathogenesis of leprosy, relating directly to the clinical form of the disease and other cytokines.
Acesso aberto (Open Access)
New experimental model of exposure to environmental tobacco smoke
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-12) BRITO, Marcus Vinicius Henriques; YASOJIMA, Edson Yuzur; SILVEIRA, Edvaldo Lima; YAMAKI, Vitor Nagai; TEIXEIRA, Renan Kleber Costa; FEIJÓ, Daniel Haber; GONÇALVES, Thiago Barbosa
PURPOSE: To describe a new model to passive smoking for rodents. METHODS: Twenty rats were distributed into two study groups (N=10): control group (CG), that was not exposed to tobacco smoke and used as normal standard for biochemical and histological analysis; Experimental Group (EG), that Animals were exposed to the passive smoking; Euthanasia was performed after 14 days of exposure. The serum level of nicotine and histological analysis were performed. RESULTS: There was a statistical difference on the nicotine serum levels between Experimental and Control group, with level of 286 ±23 nanograma/mL in the EG and undetectable on CG (p<0.01). The histological study suggested the model efficacy producing alveolar destruction and emphysema in the EG compared with the insignificant lesions in the CG's lung. CONCLUSION: The model of exposure to environmental tobacco smoke for rodents induced easily the changes related to secondhand smoke.