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Busca e classificação sistemática das proteínas oxigenases com ferro não hêmico em plantas
(Universidade Federal do Pará, 2015-03-06) SOUSA, Kellen Rayanne Matos de; DARNET, Sylvain Henri; http://lattes.cnpq.br/4586614214029929
Nonheme iron oxygenase proteins shares a conserved domain consisting of eight histidines, and they can be found in eukaryotes and prokaryotes organisms and participate in important pathways of lipid biosynthesis. To understand the evolutionary relationship among these proteins, we performed comparative and phylogenetic analyzes in prokaryotes and eukaryotes that allowed a classification of this family, nonexistent until now. The search of sequences resulted in a collection of 448 proteins, belonging to 58 organisms previously selected. The multiple alignment made with MAFFT (BLOSUM 62; L-INS-i) showed the presence of three histidine-rich motifs, with conserved spacing among them. The classification made with CLANS software has generated 28 clusters through of similarity among sequences. Two clusters contain sequences that had no similarity to proteins already characterized, and 48 sequences were not assigned to any of the 28 clusters. In the collection, 119 sequences are derived from plants, distributed in 7 clusters corresponding to C4 methysterol monooxygenase, C5 sterol desaturase, fatty acid hydroxylase, sphingolipid C4 monooxygenase, aldehyde decarbonylase, β-carotene hydroxylase and Acyl-ACP desaturase functions. Phylogenetic analysis using maximum likelihood method with PhyML tool showed the formation of well-defined groups that were similar to generated by CLANS. These results start to fill a gap existing so far about the evolutionary relationship and classification of nonheme iron oxygenases. Also, suggest that within this family there are still proteins with unknown functions, reinforcing the need for more studies of functional characterization.