Navegando por Autor "SOUZA, Alex Junior Souza de"

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Diagnóstico sorológico e caracterização molecular do vírus da hepatite E em suínos no estado do Pará, Brasil
(Universidade Federal do Pará, 2011-06-06) SOUZA, Alex Junior Souza de; CARNEIRO, Liliane Almeida; http://lattes.cnpq.br/8127606647914773; PEREIRA, Washington Luiz Assunção; http://lattes.cnpq.br/9266281009742277
Hepatitis E virus (HEV) is an RNA virus of genus Hepevirus causative agent of enterically transmitted acute hepatitis in humans that is widely distributed in many regions of the world. Pigs are reported as the main source of infection to humans related to genotypes 3 and 4 virus in non-endemic areas. In this sense, the present study aimed to demonstrate the HEV infection among swine in the Pará State by serological and molecular methods applied to samples of serum, stool and liver of 151 pigs slaughtered in metropolitan area of Belém A serologic investigation included the search for anti-HEV IgM and IgG and molecular diagnostic included the detection of the HEV RNA, nucleotide sequencing and phylogenetic analysis of sequences obtained. As result we detected no anti- HEV IgM and the prevalence of animal seroreactive for IgG was 8,6% (13/151). Molecular detection of HEV genome amplified positive fragments in 4,8% (22/453) of samples tested and the prevalence of positive animals at least one sample was 9,9% (15/151). Phylogenetic analysis has concluded that all sequences examined belonged to genotype 3 virus, described as zoonotic. We identified the subtypes 3c and 3f occurring both among samples according to the two regions of the genome amplified. These results are the first serological and molecular evidences of the existence of HEV among swine in Northern Brazil and also the first detection and genotyping of HEV in region.
Acesso aberto (Open Access)
Lesões cutâneas tipo tumorais associadas à infecção por avipoxvirus em uma marreca-cabocla (Dendrocygna autumnalis)
(2014-06) PEREIRA, Washington Luiz Assunção; GABRIEL, Áurea Martins; MONGER, Suellen da Gama Barbosa; MORAES, Leopoldo Augusto; QUEIROZ, Darlene Kássia Saraiva; SOUZA, Alex Junior Souza de
The fowlpox, also known as contagiosum epithelioma, is caused by a poxvirus of the genus Avipoxvirus and affects both domestic and wild birds. The disease has two forms cutaneous and diphtheria. In March 2008 the sector of rehabilitation of wild birds from Mangual das Garças Park, located in Belém, Pará attended a duck (Dendrocygna autumnalis) that had nodular lesions in regions of the wing devoid of feathers. The lesions consisted of two large cutaneous tumor-like nodules that measured 4,2 x 3,8 cm and 2,8 x 2,2 cm in length and width, respectively. The nodules were surgically removed, fixed in 10% formalin, processed and stained with hematoxylin-eosin. Histopathology showed acanthosis and expressive hyperkeratosis, some cells showed spongiosis. The confirmatory diagnosis of fowlpox was established by the pathognomonic sign of the presence of large intracytoplasmic eosinophilic inclusion corpuscles (Bollinger bodies) in epithelial cells. This is the first report of poxvirus infection in a wild bird in the State of Pará, Brazil.