Navegando por Autor "SOUZA, Claudia Regina Batista de"

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Extração e análise eletroforética em gel de poliacrilamida (SDS-PAGE) de proteínas totais de folhas e raízes de Piper tuberculatum
(Instituto Nacional de Pesquisas da Amazônia, 2009) SILVA, Redinaldo dos Santos; SOUZA, Claudia Regina Batista de
The Pará State is the principal Brazilian producer of black pepper (Piper nigrum Link), however, the spice’s production has been damaged by the Fusarium disease. The Fusarium solani f. sp. piperis, causal agent of this disease which attacks the plant root system promoting the root rot, leaves fall and plant death. Piperaceae plants endemic of Amazon region and resistant to F. solani f. sp. piperis infection, such as Piper tuberculatum Jacq., which has been studied in order to understand this host-pathogen interaction. In this work were evaluated five conditions for total protein extraction aiming to select suitable buffers for extraction of total proteins from P. tuberculatum leaves and roots. The conditions used to roots and leaves protein extractions were salt buffer, sucrose buffer, glycerol buffer, urea buffer and sodium phosphate buffer. Quantitative analysis showed that sucrose, glycerol and urea buffers were more efficient for leaves and roots protein extractions. SDS-PAGE analysis showed distinct band patterns in leaves and roots protein extracts obtained with different buffers. Our results could support the selection of extraction buffers for proteomics analysis in P. tuberculatum - F. solani f sp. piperis interaction studies.
Acesso aberto (Open Access)
A genomic assisted breeding program for cassava to improve nutritional quality and industrial traits of storage root
(2011-12) CARVALHO, Luiz Joaquim Castelo Branco; VIEIRA, Eduardo Alano; FIALHO, Josefino de Freitas; SOUZA, Claudia Regina Batista de
Cassava is cultivated for two ends proposals: "sweet cassava" as fresh consumes and "industry cassava" as source of starch and farina. Landraces were used to discover "spontaneous mutations" and to develop evolutionary and breeding perspective of gene function. Genomic and Proteomic resources were obtained. Gene expression by RNA blot and Microarray analysis were performed to identify differentially expressed genes. A new sugary cassava was identified to be related to missing expression of BEI and a nonsense mutation in GBSSI gene leading to amylose free starch. A pink phenotype showed no expression of CasLYB gene, and a yellow phenotype a down regulation of CasHYb. Proteomic analysis of carotenoid-protein complex together with gene expression analysis of CAP4 revealed a heteroduplex double strand cDNA associated with high carotenoid content. GBSSI gene sequencing identified 22 haplotypes and large nucleotide diversity. Segregating populations by crossing differential biochemical phenotypes and parents adapted to Cerrado's Region were obtained.
Acesso aberto (Open Access)
Studies toward the identification of transcription factors in cassava storage root
(2003-12) SOUZA, Claudia Regina Batista de; ALMEIDA, Elionor Rita Pereira de; CARVALHO, Luiz Joaquim Castelo Branco; GANDER, Eugen Silvano
Transcription factors play important roles in several physiological processes. In recent years many transcription factors have been isolated from plants and they are emerging as powerful tools in the manipulation of plant traits. In this work we initiated studies in order to isolate transcription factors from cassava (Manihot esculenta Crantz), an important tropical and subtropical crop. Our results show three kinds of proteins expressed differentially in cassava storage root and immunologically related to the opaque-2 transcription factor from maize. Southwestern experiments showed two proteins capable of interacting in vitro with the DNA sequence of the be2S1 gene from the Brazil nut tree.