Navegando por Autor "TAGLIARO, Claudia Helena"

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Acesso aberto (Open Access)
Biochemical polymorphisms and genetic relationships between Brazilian and foreign breeds of pigs reared in Brazil
(1999-06) TAGLIARO, Claudia Helena; FRANCO, Maria Helena Lartigau Pereira; SCHNEIDER, Maria Paula Cruz; BRITO, Benito Guimarães de; BARBOSA, Antonio Stockler; Centro de Ciências Rurais
The genetic variability of 14 protein systems encoded by 15 structural loci was investigated in blood samples of Piau and Caruncho pig breeds. The results were compared with those obtained previously for samples of Landrace, Large White, Duroc and Mouro. The degree of genetic variability obtained for Piau (He=0.114) was similar to that estimated for other breeds reared in Brazil (Landrace, He=0.116; Large White, He=0.119; Duroc, 0.095; Mouro, He= 0.130). Caruncho showed the lowest variability (He= 0.056). The gene frequencies at the polymorphic loci were used to evaluate the usefulness of these systems for paternity testing and the combined probabilities of paternity exclusion were estimated at 58% for the Piau and 36% for the Caruncho breed. Analysis of genetic distances revealed that the greatest similarity observed was between Piau and Landrace (D=0.042). Caruncho showed the greatest divergence among all breeds compared and the distances between this breed and others range from 0.107 (with Landrace) to 0.176 (with Duroc). The tree constructed by UPGMA and Rogers Distance gave a topology in which Piau and Mouro joined with the European breeds (Landrace and Large White) whereas Caruncho was separated from all the other breeds. The results of the analysis of the Caruncho samples should be interpreted with caution since the number of animals studied was small.
Acesso aberto (Open Access)
Genetic polymorphism and relationships among several swine populations of landrace, large white and duroc breeds
(1997-08) TAGLIARO, Claudia Helena; FRANCO, Maria Helena Lartigau Pereira; WEIMER, Tania Azevedo
The data of three protein polymorphisms were used to investigate the genetic relationships among the Landrace, Large White and Duroc swine breeds reared in Brazil, 12 other populations of these same breeds from various countries and a population of Belgium Landrace. The dendrogram, constructed from matrix of genetic distance coefficients, disclosed three large groups clustered by breed. Among them, the Landrace and the Large White showed in average closer resemblance (D = 0.203) than between them and Duroc (D = 0.241). It the three breeds, the smallest genetic distances were found between Brazilian and Cuban pig populations (Landrace: D = 0.060; Large White: D = 0.052; Duroc: D = 0.065), although there were not reports of pig exchanges between these two countries.
Acesso aberto (Open Access)
Molecular identification, phylogeny and geographic distribution of Brazilian mangrove oysters (Crassostrea)
(2010) MELO, Aline Grasielle Costa de; VARELA, Eduardo Sousa; BEASLEY, Colin Robert; SCHNEIDER, Horacio; SAMPAIO, Maria Iracilda da Cunha; GAFFNEY, Patrick Michael; REECE, Kimberly S.; TAGLIARO, Claudia Helena
Oysters (Ostreidae) manifest a high degree of phenotypic plasticity, whereby morphology is of limited value for species identification and taxonomy. By using molecular data, the aim was to genetically characterize the species of Crassostrea occurring along the Brazilian coast, and phylogenetically relate these to other Crassostrea from different parts of the world. Sequencing of the partial cytochrome oxidase c subunit I gene (COI), revealed a total of three species of Crassostrea at 16 locations along the Brazilian coast. C. gasar was found from Curuçá (Pará state) to Santos (São Paulo state), and C. rhizophorae from Fortim (Ceará state) to Florianópolis (Santa Catarina state), although small individuals of the latter species were also found at Ajuruteua beach (municipality of Bragança, Pará state). An unidentified Crassostrea species was found only on Canela Island, Bragança. Crassostrea gasar and C. rhizophorae grouped with C. virginica, thereby forming a monophyletic Atlantic group, whereas Crassostrea sp. from Canela Island was shown to be more similar to Indo-Pacific oysters, and either arrived in the Atlantic Ocean before the convergence of the Isthmus of Panama or was accidentally brought to Brazil by ship.
Acesso aberto (Open Access)
Molecular phylogeny of the genus Saguinus (Platyrrhini, Primates) based on the ND1 mitochondrial gene and implications for conservation
(2005-03) TAGLIARO, Claudia Helena; SCHNEIDER, Horacio; SAMPAIO, Maria Iracilda da Cunha; SCHNEIDER, Maria Paula Cruz; SOUZA, Marcelo Nazareno Vallinoto de; STANHOPE, Michael
The systematics of the subfamily Callitrichinae (Platyrrhini, Primates), a group of small monkeys from South America and Panama, remains an area of considerable discussion despite many investigations, there being continuing controversy over subgeneric taxonomic classifications based on morphological characters. The purpose of our research was to help elucidate the phylogenetic relationships within the monkey genus Saguinus (Callitrichinae) using a molecular approach to discover whether or not the two different sections containing hairy-faced and bare-faced species are monophyletic, whether Saguinus midas midas and Saguinus bicolor are more closely related than are S. midas midas and Saguinus midas niger, and if Saguinus fuscicollis melanoleucus and Saguinus fuscicollis weddelli really are different species. We sequenced the 957 bp ND1 mitochondrial gene of 21 Saguinus monkeys (belonging to six species and nine morphotypes) and one Cebus monkey (the outgroup) and constructed phylogenetic trees using maximum parsimony, neighbor joining, and maximum likelihood methods. The phylogenetic trees obtained divided the genus Saguinus into two groups, one containing the small-bodied species S. fuscicollis and the other, the large-bodied species S. mystax, S. leucopus, S. oedipus, S. midas, S. bicolor. The most derived taxa, S. midas and S. bicolor, grouped together, while S. fuscicollis melanoleucus and S. f. weddelli showed divergence values that did not support the division of these morphotypes into subspecies. On the other hand, S. midas individuals showed divergence compatible with the existence of three subspecies, two of them with the same morphotype as the subspecies S. midas niger. The results of our study suggest that there is at least one Saguinus subspecies that has not yet been described and that the conservation status of Saguinus species and subspecies should be carefully revised using modern molecular approaches.
Acesso aberto (Open Access)
Molecular studies of Callithrix pygmaea (Primates, Platyrrhini) based on transferrin intronic and ND1 regions: implications for taxonomy and conservation
(2000-12) TAGLIARO, Claudia Helena; SCHNEIDER, Maria Paula Cruz; SCHNEIDER, Horacio; SAMPAIO, Maria Iracilda da Cunha; STANHOPE, Michael
Traditional classifications of Platyrrhini monkeys, based mainly on morphological features, are being contested by recent molecular data. The subfamily Callitrichinae (Platyrrhini, Primates) consists of a diverse group of species, many of them considered endangered. Our analysis of two DNA regions, a mtDNA gene (ND1) and a nuclear gene (intronic regions of the transferrin gene), suggests that Callithrix pygmaea may have sufficient variability to justify the existence of subspecies or even separate species. Phylogenetic dendrograms based on the ND1 region show that this species is more closely related to Amazonian than to Atlantic forest marmosets. These results reopen the discussion about diversity and conservation programs based exclusively on traditional classifications.
Acesso aberto (Open Access)
Novel Microsatellite Markers for Brazilian Mangrove Oysters ( Crassostrea gasar ) and their Cross-Amplification in Crassostrea rhizophorae
(Universidade Federal do Pará, 2016-08) BALDEZ, Renata do Socorro Corrêa; MELO, Mauro Andre Damasceno de; SAMPAIO, Maria Iracilda da Cunha; TAGLIARO, Claudia Helena
A microsatellite CT/GT enriched genomic library was developed for Crassostrea gasar and twelve new polymorphic loci were isolated and characterized. The markers were successfully amplified from 25 individuals of Crassostrea gasar and 11 cross-amplified individuals of Crassostrea rhizophorae. There was no evidence of linkage between loci in either species.
Acesso aberto (Open Access)
Protein phenotypes and productive traits in landrace, large white and duroc swine
(1995) TAGLIARO, Claudia Helena; FRANCO, Maria Helena Lartigau Pereira; MEINCKE, Werner; SILVA, Gilberto
Fourteen protein systems coded by 15 structural loci were typed by horizontal electrophoresis to determine possible associations betweem the protein phenotypes and productive traits in Landrace (N=109), Largo White (N= 116) and Duroe (N=57) pigs, reared in Southern Brazil. Signiticant associations between protein phenotypes and production traits were detected. The most consistent interaction were observed between two protein systems (phosphogluconate dehydrogenase - Pgd and Hemopexin -Hpx) and at least one of the four performance variables considered. In Duroc breed, the Pgd phenotypes were associated with daily weight gain (P < 0.01), feed conversion ratio (P < 0.01) and selection index (P < 0.001), while in Landrace significant associations were observed only with feed convertion ratio (P < 0.05). The Hpx phenotypes were associated with daily weight gain (P < 0.05) and backfat thickness (P < 0.05) in Large White and with this last variable (P < 0.01) and selection index (P < 0.05) in Duroc pigs. Since these results had not been reported previously, turther studies are need to confirm these associations.
Acesso aberto (Open Access)
Seasonal abundance of the shipworm Neoteredo reynei (Bivalvia, Teredinidae) in mangrove driftwood from a northern Brazilian beach
(2008-03) SANTOS FILHO, Carlos; TAGLIARO, Claudia Helena; BEASLEY, Colin Robert
Shipworms are important decomposers of wood, especially in mangrove forests where productivity is high. However, little emphasis has been given to the activity of shipworms in relation to the export of nutrients from mangroves to adjacent coastal areas. As a first step to obtaining such information, the frequency of colonized mangrove driftwood as well as shipworm density and length were studied by collecting washed up logs during a year at Ajuruteua beach, state of Pará, northern Brazil. A single species, Neoteredo reynei (Bartsch, 1920), was found colonizing driftwood. Although large colonized logs were most common on the beach, shipworm density was higher in small logs, especially during the dry season. In general, however, density was higher during the wet season (January to April) and lowest in July. Overall shipworm mean length was 9.66cm. In large logs, mean length increased between the wet and dry seasons. However, there was no difference in length among log size categories. Mean shipworm length was similar throughout most of the year but tended to be greater in July. Although salinity varied between 10.9 and 40 during the year, no relationship was found between salinity and density or length. The results suggest that shipworm activity in driftwood logs is relatively constant throughout the year. Increased air humidity and rainfall may promote survival during the wet season. Large logs may take longer to colonize and thus have lower densities than small ones which are scarce probably because they are destroyed rapidly by shipworm activity. However, data on the disintegration of logs would be necessary to test this hypothesis. Larger size of shipworms in the dry season may be related to growth after an earlier recruitment period. Shipworms in large logs during the dry season may be better protected from dessication and high temperatures by the insulating properties of the larger volume of wood.
Acesso aberto (Open Access)
Significant genetic differentiation among populations of Anomalocardia brasiliana (Gmelin, 1791): a bivalve with planktonic larval dispersion
(2009) ARRUDA, Cinthya Cristina Bulhões; BEASLEY, Colin Robert; SOUZA, Marcelo Nazareno Vallinoto de; SILVA, Nelane do Socorro Marques da; TAGLIARO, Claudia Helena
Four Brazilian populations of Anomalocardia brasiliana were tested for mutual genetic homogeneity, using data from 123 sequences of the mtDNA cytochrome oxidase c subunit I gene. A total of 36 haplotypes were identified, those shared being H3 (Canela Island, Prainha and Acupe) and both H5 and H9 (Prainha and Acupe). Haplotype diversity values were high, except for the Camurupim population, whereas nucleotide values were low in all the populations, except for that of Acupe. Only the Prainha population showed a deviation from neutrality and the SSD test did not reject the demographic expansion hypothesis. Fst values showed that the Prainha and Acupe populations represent a single stock, whereas in both the Canela Island and Camurupim stocks, population structures are different and independent. The observed structure at Canela Island may be due to the geographic distance between this population and the remainder. The Camurupim population does not share any haplotype with the remaining populations in northeastern Brazil. The apparent isolation could be due to the rocky barrier located facing the mouth of the Mamanguape River. The results highlight the importance of wide-scale studies to identify and conserve local genetic diversity, especially where migration is restricted.
Acesso aberto (Open Access)
Taxonomic implications of molecular studies on Northern Brazilian Teredinidae (Mollusca: Bivalvia) specimens
(2005-03) VALE, Sonia Maria Lima Santos do; TAGLIARO, Claudia Helena; BEASLEY, Colin Robert; SCHNEIDER, Horacio; SAMPAIO, Maria Iracilda da Cunha; SANTOS FILHO, Carlos; MÜLLER, Ana Cláudia de Paula
The current taxonomy of the Teredinidae (shipworms) is wholly based on morphology and up to now no molecular studies of the phylogeny of this group have been published. In the present study the relationships between four genera of the subfamilies Teredininae and Bankiinae were established and the efficiency of the 16S rRNA gene in characterizing four Teredinidae species was tested. Phylogenetic trees support the grouping of Bankia fimbriatula with Nausitora fusticula and of Neoteredo reynei with Psiloteredo healdi, but the genetic distances do not justify the classification of these species into two distinct subfamilies. The results show that B. fimbriatula, N. reynei and P. healdi specimens from the coast of the Brazilian state of Pará have five distinct 16S rRNA haplotypes, with one N. reynei haplotype differing from the other haplotypes in respect to at least seven sequences sites, indicating the existence of two very distinct sympatric lineages.
Acesso aberto (Open Access)
Tocantins river as an effective barrier to gene flow in Saguinus niger populations
(2006) SOUZA, Marcelo Nazareno Vallinoto de; SILVA, Juliana Araripe Gomes da; RÊGO, Péricles Sena do; TAGLIARO, Claudia Helena; SAMPAIO, Maria Iracilda da Cunha; SCHNEIDER, Horacio
The Saguinus represent the basal genus of the Callitrichinae subfamily. Traditionally this genus is divided into three groups: Hairy, Mottled and Bare-face, however, molecular data failed to validate these groups as monophyletic units, as well as raised some subspecies to the species status. This is the case of the former subspecies Saguinus midas midas and S. midas niger, which are now considered as different species. In the present study, we sequenced a portion of the D-loop mtDNA region in populations from the East bank of the Xingu and from both banks of the Tocantins river, in order to test the effectiveness of large rivers as barriers to the gene flow in Saguinus. According to our results, the populations from the East and West banks of the Tocantins river are more divergent than true species like S. mystax and S. imperator. The Tocantins river may be acting as a barrier to gene flow, and consequently these very divergent populations may represent distinct taxonomic entities (species?).