Navegando por Autor "TEIXEIRA, Renan Kleber Costa"

Agora exibindo 1 - 7 de 7

Acesso aberto (Open Access)
Copaiba oil effect under different pathways in mice subjected to sepsis
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-08) BOTELHO, Nara Macedo; SILVEIRA, Edvaldo Lima; LOPES, Letícia Nobre; SANTOS, Felipe Augusto Folha; TEIXEIRA, Renan Kleber Costa; SILVA, Thaís Travassos da
PURPOSE: To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture. METHODS: Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals; Control group: submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed. The Kaplan-Meier curves of surviving time were realized. RESULTS: All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05). CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage.
Acesso aberto (Open Access)
Effect of andiroba oil on periodontitis in Wistar rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-06) CARMONA, Glaucia Babeto; TEIXEIRA, Renan Kleber Costa; BRITO, Marcus Vinicius Henriques; PONTES, Flávia Sirotheau Corrêa; ANDRADE, Eloisa Helena de Aguiar; FONSECA, Felipe Paiva; COSTA, Ricardo Miranda Brito; CARVALHO, Francideise Martins
PURPOSE: To evaluate the effects of andiroba oil on the periodontitis in rats. METHODS: The periodontitis was induced by the placement of cotton ligatures around the cervix of the second upper molars on fifteen rats, and waiting fifty days. The animals were randomly distributed into three groups: saline group, andiroba oil group and meloxican group, differentiated by substance used in the treatment of periodontitis. The groups received the respective substance by gavage for seven days, after the periodontitis induced. It was analyzed the score of inflammatory cells and the measurement from the cemento-enamel junction to the bone crest. RESULTS: The andiroba oil group (p=0.008) and meloxican group (p=0.0347) show a less score of inflammatory cells than saline group, however there weren't difference between them (p=0.2754). Regarding the analysis of measurement from the cemento-enamel junction to the bone crest, there was no difference between groups studied (p=0.3451). CONCLUSION: Andiroba oil decreased the quantity of inflammatory cells, however, it didn't have an effect on the measurement of alveolar bone loss, like the treatment with Meloxican®.
Acesso aberto (Open Access)
Effect of copaiba oil in hepatic damage induced by acetaminophen in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-07) TEIXEIRA, Renan Kleber Costa; YAMAKI, Vitor Nagai; YASOJIMA, Edson Yuzur; BRITO, Marcus Vinicius Henriques
PURPOSE: To investigate the effects of copaiba oil on the hepatic damage induced by paracetamol. METHODS: Thirty six rats were distributed into six study groups (N=6): control group, that didn't receive the acetaminophen; Acetaminophen Group, that only received the acetaminophen; Prophylactic Copaiba Group 1, that received copaiba oil two hours before the acetaminophen; Prophylactic Copaiba Group 7, that received copaiba oil seven days, once by day, before the acetaminophen; Therapy Copaiba Group, that received the copaiba oil two hours afther the acetaminophen; and N-Acetyl-Cysteine Group, , that received the N-Acetyl-Cysteine two hours afther the acetaminophen. Euthanasia was performed after 24 hours. The serum levels of AST, ALT, alkaline phosphatase, GT, total bilirubin, direct bilirubin and indirect bilirubin and histological analisis were analized. RESULTS: The prophylactic copaiba group 7, therapy copaiba group and N-Acetyl-Cysteine Group showed amounts of AST and ALT similar to the control group; and the prophylactic copaiba group 1 showed similar levels to the acetaminophen group. There was no significant difference between the groups regarding the amount of alkaline phosphatase and GT (p>0.05). The therapy copaiba group showed the highest levels of bilirubin and was statistically different from the other groups (p<0.01) and this increased the costs of direct bilirubin. Regarding histopathology, the oil of copaiba administered prophylactic or therapeutic form for 7 days could decrease the amount of necrosis and inflammatory infiltrate. CONCLUSION: Copaiba oil administered prophylactically for seven days, and therapeutic could reduce liver damage caused by paracetamol similarly N-Acetyl-Cysteine, however, when treated with copaiba therapeutically showed increases in bilirubin, costs increasing fraction indirect.
Acesso aberto (Open Access)
Immunohistochemistry of the uterine cervix of rats bearing the Walker 256 tumor treated with copaiba balsam
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-03) BOTELHO, Nara Macedo; CORRÊA, Suelen Costa; LOBATO, Rodolfo Costa; TEIXEIRA, Renan Kleber Costa; QUARESMA, Juarez Antônio Simões
PURPOSE: To investigate the immunohistochemistry of the uterine cervix of 20 Wistar rats (Rattus norvegicus) bearing the Walker 256 tumor, treated with copaiba oil (Copaifera officinalis). METHODS: The animals were grouped into four subgroups, with five rats each: the GCT and GCopT received distilled water and topically copaiba, respectively, while the GCG and GCopG received distilled water and copaiba by gavage, respectively. The substances were administered for nine days. On the 12th day, after euthanasia, the tumor pieces were sent to the identification of T CD4+, T CD8+ and Natural Killer cells. RESULTS: It was found that the pattern of expression for specific markers of phenotypes of cells involved in tumor immune response was similar in all groups, regardless the administration way of copaiba oil (topical or gavage). CONCLUSION: Copaiba balsam, administered either topically or by gavage, did not alter the pattern of tumor immune response in rats bearing Walker 256 Tumor.
Acesso aberto (Open Access)
Influence of oophorectomy on glycemia and lipidogram
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2018-05) TEIXEIRA, Renan Kleber Costa; FEIJÓ, Daniel Haber; VALENTE, André Lopes; CARVALHO, Luan Teles Ferreira de; GRANHEN, Hícaro Donato; PETROIANU, Andy; BOTELHO, Nara Macedo
Methods:Fifty six female rats were divided into the following 7 groups (n = 8): group 1 - sham group, group 2 - oophorectomy 30 days, group 3 - oophorectomy 35 days, group 4 - oophorectomy 40 days, group 5 - oophorectomy 45 days, group 6 - oophorectomy 70 days, group 7 - oophorectomy 55 days. Animals were following by number of days according the group. Was evaluated the serum levels of glucose and lipid profile. Results: The oophorectomized rats presented higher glycemia. Groups 3, 4, 6 and 7 had a higher glycemia and LDL levels (except for group 6) and groups 6 and 7 had lowest levels of HDL. Group 7 had highest level of VLDL than oophorectomy groups. There was no difference in triglycerides levels. Conclusion: Oophorectomy was related to dyslipidemia and insulin resistance, mainly after 50th days.
Acesso aberto (Open Access)
Influence of tramadol on functional recovery of acute spinal cord injury in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2018-12) CHAVES, Rosa Helena de Figueiredo; SOUZA, Celice Cordeiro de; FURLANETO, Ismari Perini; TEIXEIRA, Renan Kleber Costa; OLIVEIRA, Carolina Pinheiro de; RODRIGUES, Emanuelle de Matos; SANTOS, Daniel Arthur Santos dos; SILVA, Renata Cunha; PENHA, Nelson Elias Abrahão da; LIMA, Ana Rita de
Purpose: To evaluate the influence tramadol on functional recovery of acute spinal cord injury in rats. Methods: Ten rats were divided into two groups (n = 5). All animals were submitted by a aminectomy and spinal cord injury at eighth thoracic vertebra. In control group, the rats didn’t receive any analgesic. In tramadol group, the rats received tramadol 4mg/Kg at 12/12h until 5 days by subcutaneous. Animals were following by fourteen days. Was evaluated the Basso, Beattie, Bresnahan scale (locomotor evaluation) and Rat Grimace Scale (pain evaluation) at four periods. Results: There no difference between the groups in locomotor evaluation in all periods evaluated (p>0.05) and in both groups there was a partial recover of function. The tramadol group show a lower pain levels at the first, third and seventh postoperatively days when comparing to the control group. Conclusion: The tramadol as an analgesic agent don’t influence on functional recovery of acute spinal cord injury in rats.
Acesso aberto (Open Access)
New experimental model of exposure to environmental tobacco smoke
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-12) BRITO, Marcus Vinicius Henriques; YASOJIMA, Edson Yuzur; SILVEIRA, Edvaldo Lima; YAMAKI, Vitor Nagai; TEIXEIRA, Renan Kleber Costa; FEIJÓ, Daniel Haber; GONÇALVES, Thiago Barbosa
PURPOSE: To describe a new model to passive smoking for rodents. METHODS: Twenty rats were distributed into two study groups (N=10): control group (CG), that was not exposed to tobacco smoke and used as normal standard for biochemical and histological analysis; Experimental Group (EG), that Animals were exposed to the passive smoking; Euthanasia was performed after 14 days of exposure. The serum level of nicotine and histological analysis were performed. RESULTS: There was a statistical difference on the nicotine serum levels between Experimental and Control group, with level of 286 ±23 nanograma/mL in the EG and undetectable on CG (p<0.01). The histological study suggested the model efficacy producing alveolar destruction and emphysema in the EG compared with the insignificant lesions in the CG's lung. CONCLUSION: The model of exposure to environmental tobacco smoke for rodents induced easily the changes related to secondhand smoke.