Navegando por Autor "TURK, Meritxell Zurita"

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Acesso aberto (Open Access)
Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains
(Public Library of Science, 2011-04) RUIZ, Jeronimo Conceição; FERREIRA, Vívian D'Afonseca da Silva; SILVA, Artur Luiz da Costa da; ALI, Amjad; GOMIDE, Anne Cybelle Pinto; SANTOS, Anderson Rodrigues dos; ROCHA, Aryane Aparecida Magalhães Cassiano; DORELLA, Fernanda Alves; PACHECO, Luis Gustavo Carvalho; COSTA, Marcilia Pinheiro da; TURK, Meritxell Zurita; SEYFFERT, Núbia; MORAES, Pablo Matias Ribeiro de Oliveira; SOARES, Siomar de Castro; ALMEIDA, Sintia Silva de; CASTRO, Thiago Luiz de Paula; ABREU, Vinicius Augusto Carvalho de; TROST, Eva; BAUMBACH, Jan; TAUCH, Andreas; SCHNEIDER, Maria Paula Cruz; MCCULLOCH, John Anthony; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; ZERLOTINI NETO, Adhemar; DOMINITINI, Anderson Joaquim; RESENDE, Daniela de Melo; COSER, Elisângela Monteiro; OLIVEIRA, Luciana Márcia de; PEDROSA, André Luiz; VIEIRA, Carlos Ueira; GUIMARÃES, Claudia Teixeira; BARTHOLOMEU, Daniela C.; OLIVEIRA, Diana Magalhães de; SANTOS, Fabrício Rodrigues dos; RABELO, Elida Mara Leite; LOBO, Francisco Pereira; FRANCO, Gloria Regina; COSTA, Ana Flávia Dias Vieira da
Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity.
Acesso aberto (Open Access)
The Corynebacterium pseudotuberculosis in silico predicted pan-exoproteome
(BioMed Central Ltd, 2012) SANTOS, Anderson Rodrigues dos; CARNEIRO, Adriana Ribeiro; GALA-GARCÍA, Alfonso; GOMIDE, Anne Cybelle Pinto; BARH, Debmalya; BARBOSA, Eudes Guilherme Vieira; ABURJAILE, Flavia Figueira; DORELLA, Fernanda Alves; ROCHA, Flávia de Souza; GUIMARÃES, Luís Carlos; TURK, Meritxell Zurita; RAMOS, Rommel Thiago Juca; ALMEIDA, Sintia Silva de; SOARES, Siomar de Castro; PEREIRA, Ulisses de Pádua; ABREU, Vinicius Augusto Carvalho de; SILVA, Artur Luiz da Costa da; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho
Background: Pan-genomic studies aim, for instance, at defining the core, dispensable and unique genes within a species. A pan-genomics study for vaccine design tries to assess the best candidates for a vaccine against a specific pathogen. In this context, rather than studying genes predicted to be exported in a single genome, with pan genomics it is possible to study genes present in different strains within the same species, such as virulence factors. The target organism of this pan-genomic work here presented is Corynebacterium pseudotuberculosis, the etiologic agent of caseous lymphadenitis (CLA) in goat and sheep, which causes significant economic losses in those herds around the world. Currently, only a few antigens against CLA are known as being the basis of commercial and still ineffective vaccines. In this regard, the here presented work analyses, in silico, five C. pseudotuberculosis genomes and gathers data to predict common exported proteins in all five genomes. These candidates were also compared to two recent C. pseudotuberculosis in vitro exoproteome results. Results: The complete genome of five C. pseudotuberculosis strains (1002, C231, I19, FRC41 and PAT10) were submitted to pan-genomics analysis, yielding 306, 59 and 12 gene sets, respectively, representing the core, dispensable and unique in silico predicted exported pan-genomes. These sets bear 150 genes classified as secreted (SEC) and 227 as potentially surface exposed (PSE). Our findings suggest that the main C. pseudotuberculosis in vitro exoproteome could be greater, appended by a fraction of the 35 proteins formerly predicted as making part of the variant in vitro exoproteome. These genomes were manually curated for correct methionine initiation and redeposited with a total of 1885 homogenized genes. Conclusions: The in silico prediction of exported proteins has allowed to define a list of putative vaccine candidate genes present in all five complete C. pseudotuberculosis genomes. Moreover, it has also been possible to define the in silico predicted dispensable and unique C. pseudotuberculosis exported proteins. These results provide in silico evidence to further guide experiments in the areas of vaccines, diagnosis and drugs. The work here presented is the first whole C. pseudotuberculosis in silico predicted pan-exoproteome completed till today.