Navegando por Autor "YAMASAKI, Elise Miyuki"

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Intoxicação natural e experimental por Metternichia princeps (Solanaceae) em caprinos
(2012-09) PRADO, Juliana da Silva; BRITO, Marilene de Farias; CALDAS, Saulo Andrade; YAMASAKI, Elise Miyuki; BARBOSA NETO, José Diomedes; MAIA, Diogo dos Santos; TOKARNIA, Carlos Maria Antônio Hubinger
From 2007 to 2009 a nephrotoxic disease of subacute course and high mortality in goats occurred on a farm in the county of Itaguaí, Rio de Janeiro. Metternichia princeps, a plant of the Solanaceae family, was suspected to be the cause. Through experiments in goats the clinical-pathological picture of poisoning by the plant and the lethal dose were established. For the experiments 12 young and adult goats of different races, both sexes and with weights above 15 kg were used. The goats that received 30g/kg in 5 days, 15g/kg in 3 days, single doses of 10g/kg and 5g/kg, all died. Of two goats that received single doses of 2.5g/kg, one died but the other did not show any clinical sign; a goat that received the single dose of 1.25g/kg also did not show any symptom of poisoning. First clinical signs were observed from 7 hours to 46h45min after the plant intake. The clinical course varied from 3h6min to 126h40min. First clinical signs were loss of appetite, adipsia, apathy and reluctance to move. After the animals entered in sternal decubitus and when they were placed in standing position, they kept their front limbs flexed, supported only by the posterior limbs on the floor until evolving into flexion of all four limbs followed by lateral decubitus. Postmortem examination revealed edema of the perirenal adipose tissue, pale kidneys which on the cut-surface showed whitish stripes from the cortex to the medullar area. At histopathology, severe coagulative necrosis of epithelial cells of the kidney tubules was seen. Compared with the natural cases, the goats experimentally poisoned by M. princeps, presented a similar clinic-pathological picture. By these experiments it could be proved that Metternichia princeps is the toxic plant that causes a nephrotoxic disease in goats in the Rio de Janeiro area; the minor lethal dose was 2,5g/kg in the experiments.
Acesso aberto (Open Access)
Intoxicação por Cestrum laevigatum (Solanaceae) em bubalinos
(2010-12) BARBOSA NETO, José Diomedes; OLIVEIRA, Carlos Magno Chaves; PINHEIRO, Cleyton Prado; LOPES, Cinthia Távora de Albuquerque; MARQUES, Débora Maria Marquiori; BRITO, Marilene de Farias; YAMASAKI, Elise Miyuki; TOKARNIA, Carlos Maria Antônio Hubinger
Based on the history and clinical and pathological data, as well as on inspection of the pastures, a mortality of buffaloes in the county of Itaguaí/RJ, Brazil, was diagnosed as poisoning by Cestrum laevigatum Schlecht., a plant of the Solanaceae family. The poisoning was reproduced in two buffaloes. Dried leaves of the shrub were administered by hand, in single doses corresponding to 20g/kg and 40g/kg of the fresh leaves, to four buffaloes of the Murrah breed. The dose corresponding to 40g/kg of the fresh leaves caused signs of poisoning, as apathy, anorexia, absence of rumen movements, dysmetria, excitement and aggressiveness, followed by death of the two buffaloes within 65 hours after administration. From the two buffaloes that received the corresponding dose of 20g/kg of the fresh plant, one presented clinical signs characterized mainly by decrease of the rumen movements, but recovered 97h22min after the administration; the other buffalo did not show symptoms of poisoning. Laboratory analyses for biochemical evaluation accused hepatic alterations. In one buffalo that died, the main macroscopic finding was an orange liver with a clear nutmeg appearance; in the second buffalo, the orange liver had no nutmeg appearance. Other alterations found in these two buffaloes were slight edema of the gall bladder wall, a slightly reddish mucous membrane of the abomasum, extensive echymoses in the endocard of the left ventricle and few petechiae in the endocard of the right ventricle; the abomasum content was slightly dry, and the large intestine had little and slightly dry contents wrapped by mucus. Histopatological examination revealed severe coagulative necrosis of the liver parenchyma in the centrolobular and intermediate lobular areas, with a halo of vacuolated hepatocytes at the periphery of the necrotic areas.
Acesso aberto (Open Access)
Lesões traumáticas de pele causadas pelos espinhos de Mimosa pudica e Mimosa debilis em equídeos
(2011-09) REIS, Alessandra dos Santos Belo; DUARTE, Marcos Dutra; SOUSA, Melina Garcia Saraiva de; FREITAS, Nayra Fernanda de Queiroz Ramos; YAMASAKI, Elise Miyuki; SILVA, André Guimarães Maciel e; OLIVEIRA, Carlos Magno Chaves; BARBOSA NETO, José Diomedes
Studies on the nature and cause of skin lesions in horses on a farm in the county of Castanhal, northeastern Para, Brazil were conducted. These were visits to the farm, epidemiological studies, blood sample collections, skin biopsies, and inspection of the pasture. The study included 25 Equidae, 14 males and 11 females, six months to eight years old. The animals showed ulcerative lesions of irregular borders on the head (nose, muzzle, upper and lower lips), in the oral cavity (buccal vestibule and gum) and on the limbs (billets, metacarpals and metatarsals and scapular-humeral joint). The histopathological examination revealed foci of cutaneous erosions with epidermal necrosis, spongiosis and vesicular degeneration of the remaining epidermis, and mild inflammatory infiltrate in the underlying dermis, consisting predominantly of macrophages and, to a lesser degree, eosinophils. The inspection of the pasture, which consisted of Brachiaria humidicola, revealed a heavy invasion by two plants provided with spines, Mimosa pudica and Mimosa debilis, of the Leguminosae Mimosoideae family. It was concluded that the skin lesions were caused by traumatic action of the spines of Mimosa pudica and Mimosa debilis.
Acesso aberto (Open Access)
Surto de encefalomielite equina Leste na Ilha de Marajó, Pará
(2013-04) CAMPOS, Karinny Ferreira; OLIVEIRA, Cairo Henrique Sousa de; REIS, Alessandra dos Santos Belo; YAMASAKI, Elise Miyuki; BRITO, Marilene de Farias; ANDRADE, Stefano Juliano Tavares de; DUARTE, Marcos Dutra; BARBOSA NETO, José Diomedes
Nine cases of equine encephalomyelitis on Marajó Island, state of Pará, Brazil, were studied. The affected horses had difficulty to stand, walked in circles, with marked depression, closed eyelids, tongue paralysis, muscle tremors, bruxism, anorexia and dehydration. Some had their ear and eyelid reflexes diminished, decreased tongue tone and tachycardia; laid down frequently they kept their head on the chest. Often they were seen resting their head on tree trunks or fences. At necropsy, hemorrhages of the meninges and spinal cord, and in some animals also adhesion of the meninges were found. Histologically there was diffuse encephalitis affecting mainly the gray matter, with meningitis and choroiditis. Presence of perivascular cuffs consisting of mononuclear inflammatory cells was observed. From two horses the Eastern equine encephalitis virus was identified by semi-nested reverse transcription polymerase chain reaction (semi-nested RT-PCR).