Navegando por Autor "YASOJIMA, Edson Yuzur"
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Item Acesso aberto (Open Access) Effect of copaiba oil in hepatic damage induced by acetaminophen in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-07) TEIXEIRA, Renan Kleber Costa; YAMAKI, Vitor Nagai; YASOJIMA, Edson Yuzur; BRITO, Marcus Vinicius HenriquesPURPOSE: To investigate the effects of copaiba oil on the hepatic damage induced by paracetamol. METHODS: Thirty six rats were distributed into six study groups (N=6): control group, that didn't receive the acetaminophen; Acetaminophen Group, that only received the acetaminophen; Prophylactic Copaiba Group 1, that received copaiba oil two hours before the acetaminophen; Prophylactic Copaiba Group 7, that received copaiba oil seven days, once by day, before the acetaminophen; Therapy Copaiba Group, that received the copaiba oil two hours afther the acetaminophen; and N-Acetyl-Cysteine Group, , that received the N-Acetyl-Cysteine two hours afther the acetaminophen. Euthanasia was performed after 24 hours. The serum levels of AST, ALT, alkaline phosphatase, GT, total bilirubin, direct bilirubin and indirect bilirubin and histological analisis were analized. RESULTS: The prophylactic copaiba group 7, therapy copaiba group and N-Acetyl-Cysteine Group showed amounts of AST and ALT similar to the control group; and the prophylactic copaiba group 1 showed similar levels to the acetaminophen group. There was no significant difference between the groups regarding the amount of alkaline phosphatase and GT (p>0.05). The therapy copaiba group showed the highest levels of bilirubin and was statistically different from the other groups (p<0.01) and this increased the costs of direct bilirubin. Regarding histopathology, the oil of copaiba administered prophylactic or therapeutic form for 7 days could decrease the amount of necrosis and inflammatory infiltrate. CONCLUSION: Copaiba oil administered prophylactically for seven days, and therapeutic could reduce liver damage caused by paracetamol similarly N-Acetyl-Cysteine, however, when treated with copaiba therapeutically showed increases in bilirubin, costs increasing fraction indirect.Item Acesso aberto (Open Access) Effects of hypertonic saline solution associated to remote ischemic perconditioning in kidney ischemia/reperfusion injury in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2017-03) BRITO, Marcus Vinicius Henriques; YASOJIMA, Edson Yuzur; PÉRCARIO, Sandro; RIBEIRO JUNIOR, Rubens Fernando Gonçalves; CAVALCANTE, Lainy Carollyne da Costa; MONTEIRO, Andrew Moraes; COUTEIRO, Rodrigo Paracampo; RODRIGUES, Ivone Aline da Silva; SANTOS, Hellen Aparecida Geyer dosTo evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. Results: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. Conclusion:Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.Item Acesso aberto (Open Access) New experimental model of exposure to environmental tobacco smoke(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-12) BRITO, Marcus Vinicius Henriques; YASOJIMA, Edson Yuzur; SILVEIRA, Edvaldo Lima; YAMAKI, Vitor Nagai; TEIXEIRA, Renan Kleber Costa; FEIJÓ, Daniel Haber; GONÇALVES, Thiago BarbosaPURPOSE: To describe a new model to passive smoking for rodents. METHODS: Twenty rats were distributed into two study groups (N=10): control group (CG), that was not exposed to tobacco smoke and used as normal standard for biochemical and histological analysis; Experimental Group (EG), that Animals were exposed to the passive smoking; Euthanasia was performed after 14 days of exposure. The serum level of nicotine and histological analysis were performed. RESULTS: There was a statistical difference on the nicotine serum levels between Experimental and Control group, with level of 286 ±23 nanograma/mL in the EG and undetectable on CG (p<0.01). The histological study suggested the model efficacy producing alveolar destruction and emphysema in the EG compared with the insignificant lesions in the CG's lung. CONCLUSION: The model of exposure to environmental tobacco smoke for rodents induced easily the changes related to secondhand smoke.Item Acesso aberto (Open Access) Stem cells from adipose tissue improve the time of wound healing in rats(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2016-12) OHASHI, Camila Melo; CALDEIRA, Fabio Alves Morikawa; FEITOSA JUNIOR, Denilson José Silva; VALENTE, André Lopes; DUTRA, Paulo Roberto Witter; MIRANDA, Moysés dos Santos; SANTOS, Simone do Socorro Damasceno; BRITO, Marcus Vinicius Henriques; OHASHI, Otávio Mitio; YASOJIMA, Edson YuzurPURPOSE: To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS: Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS: All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions. CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.