Navegando por Autor "ZERLOTINI NETO, Adhemar"

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Acesso aberto (Open Access)
Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains
(Public Library of Science, 2011-04) RUIZ, Jeronimo Conceição; FERREIRA, Vívian D'Afonseca da Silva; SILVA, Artur Luiz da Costa da; ALI, Amjad; GOMIDE, Anne Cybelle Pinto; SANTOS, Anderson Rodrigues dos; ROCHA, Aryane Aparecida Magalhães Cassiano; DORELLA, Fernanda Alves; PACHECO, Luis Gustavo Carvalho; COSTA, Marcilia Pinheiro da; TURK, Meritxell Zurita; SEYFFERT, Núbia; MORAES, Pablo Matias Ribeiro de Oliveira; SOARES, Siomar de Castro; ALMEIDA, Sintia Silva de; CASTRO, Thiago Luiz de Paula; ABREU, Vinicius Augusto Carvalho de; TROST, Eva; BAUMBACH, Jan; TAUCH, Andreas; SCHNEIDER, Maria Paula Cruz; MCCULLOCH, John Anthony; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; ZERLOTINI NETO, Adhemar; DOMINITINI, Anderson Joaquim; RESENDE, Daniela de Melo; COSER, Elisângela Monteiro; OLIVEIRA, Luciana Márcia de; PEDROSA, André Luiz; VIEIRA, Carlos Ueira; GUIMARÃES, Claudia Teixeira; BARTHOLOMEU, Daniela C.; OLIVEIRA, Diana Magalhães de; SANTOS, Fabrício Rodrigues dos; RABELO, Elida Mara Leite; LOBO, Francisco Pereira; FRANCO, Gloria Regina; COSTA, Ana Flávia Dias Vieira da
Corynebacterium pseudotuberculosis, a Gram-positive, facultative intracellular pathogen, is the etiologic agent of the disease known as caseous lymphadenitis (CL). CL mainly affects small ruminants, such as goats and sheep; it also causes infections in humans, though rarely. This species is distributed worldwide, but it has the most serious economic impact in Oceania, Africa and South America. Although C. pseudotuberculosis causes major health and productivity problems for livestock, little is known about the molecular basis of its pathogenicity.
Acesso aberto (Open Access)
Reannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria
(Dove Medical Press, 2012-02) FERREIRA, Vívian D'Afonseca da Silva; SOARES, Siomar de Castro; ALI, Amjad; SANTOS, Anderson Rodrigues dos; GOMIDE, Anne Cybelle Pinto; ROCHA, Aryane Aparecida Magalhães Cassiano; FARIA, Cássio de Jesus; BARBOSA, Eudes Guilherme Vieira; GUIMARÃES, Luís Carlos; ESLABÃO, Marcus Redü; ALMEIDA, Sintia Silva de; ABREU, Vinicius Augusto Carvalho de; ZERLOTINI NETO, Adhemar; CARNEIRO, Adriana Ribeiro; CERDEIRA, Louise Teixeira; RAMOS, Rommel Thiago Juca; HIRATA JÚNIOR, Raphael; GUARALDI, Ana Luiza de Mattos; TROST, Eva; TAUCH, Andreas; SILVA, Artur Luiz da Costa da; SCHNEIDER, Maria Paula Cruz; MIYOSHI, Anderson; AZEVEDO, Vasco Ariston de Carvalho
Background: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism’s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other spe cies of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as “hypothetical proteins,” now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm. icb.ufmg.br/pub/C_diphtheriae_reannotation.embl.