Programa de Pós-Graduação em Química - PPGQ/ICEN
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/4046
O Programa de Pós-Graduação em Química (PPGQ) do Instituto de Ciências Exatas e Naturais (ICEN) da Universidade Federal do Pará (UFPA). Oferece oportunidade para a formação de Mestres e Doutores nas áreas de Química Orgânica, Físico-Química, Inorgânica e Analítica.
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Item Acesso aberto (Open Access) Estudo fitoquímico e avaliação do potencial antimicrobiano e antioxidadnte das folhas de metrodorea flavida k. krause(Universidade Federal do Pará, 2023-09-29) CONCEIÇÃO, Carlos Maurício Nunes de Souza da; PINHEIRO, Wandson Braamcamp de Souza; http://lattes.cnpq.br/8867866033296703; SANTOS, Lourivaldo da Silva; http://lattes.cnpq.br/3232898465948962; SILVA, Milton Nascimento da; http://lattes.cnpq.br/6742390457977989; https://orcid.org/0000-0001-9755-4058The species Metrodorea flavida K. Krause, popularly known as three leaves, belongs to the Rutaceae family, and is an endemic species of the Amazon biome. Despite belonging to a taxon of economic and medicinal relevance, in addition to being located in one of the regions of the planet with the greatest biotechnological relevance, there is still a scarcity of studies focused on the phytochemistry of M. flavida for this species. Therefore, this work sought to contribute to the phytochemical study of this species, through the determination of the chemical, chromatographic and spectroscopic profiles of M. flavida extracts, the isolation and structural elucidation of the major compounds, as well as the evaluation of antioxidant potentials. and antimicrobial from M. flavida leaf extracts. Firstly, microextractions were carried out from the plant leaves with the solvents ethyl acetate, 96% ethanol and 70% ethanol. After analyzing the profiles obtained by High Efficiency Thin Layer Chromatography, High Efficiency Liquid Chromatography (HPLC), by Nuclear Magnetic Resonance and antioxidant and antimicrobial tests with the extracts, an extraction was carried out by maceration using 70% ethanol, of this extract. fractionation occurred with Solid Phase Extraction, resulting in 23 fractions, of which the constituents of fraction 60%V1 were purified by high-performance liquid chromatography, as well as the isolated substances were elucidated by 1D Nuclear Magnetic Resonance (NMR) spectroscopy and 2D. The chemical profiles of the M. flavida extracts indicated the presence of substances from the classes of terpenes, flavonoids and coumarins, the antioxidant tests of the extracts demonstrated that the microextract obtained with 70% ethanol presented greater efficiency in scavenging DPPH• radicals, in addition, for antimicrobial tests, the microextract obtained with ethyl acetate showed the best results for strains of Staphylococcus aureus, Bacillus subtilis and Escherichia coli. The 70% ethanol macerated extract was also tested for the same strains mentioned above and showed bactericidal action for S. aureus, B. subtilis and E. coli, in addition to bacteriostatic action for Salmonella typhimurium. From the 60%V1 fraction, five substances were isolated (S1, S2, S3, S4 and S5), which after analysis by NMR and thorough research on the Scinfinder and PubChem platforms, it was found that substances S2 and S4 are treated of two new dihydrochalcones, isolated for the first time from plants. In this way, the data obtained represent a contribution to the study of phytochemistry and the biological properties of the species M. flavida, as well as to the natural product chemistry, since two new natural compounds were discovered.Item Acesso aberto (Open Access) Investigação de metabólitos secundários de folhas de Derris urucu (Killip et Smith) Macbr. com atividades biológicas(Universidade Federal do Pará, 2009-06-30) LÔBO, Lívia Trindade; SILVA, Milton Nascimento da; http://lattes.cnpq.br/6742390457977989; ARRUDA, Mara Silvia Pinheiro; http://lattes.cnpq.br/8047078292358267Derris urucu, belonging to the family Leguminosae / Fabaceae, is popularly known as “timbó”. The roots of this species are commonly used as pesticides and poison to fish. From Derris genus many phytochemical studies have been reported, the roots being of the most studied part from the plants of this genus. The name “timbó” is most general for the species Derris urucu and Derris nicou, which are the species that produce, in their roots, rotenoids such as rotenone and deguelin, from which derives the importance of these plants. The extracts, and the substances isolated from this genus are responsible for a wide range of biological activities, mainly the insecticidal activity. From the ethanolic extract of leaves of D. urucu, twelve compounds were isolated and purified by High Performance Liquid Chromatography: five stilbenes, six dihydroflavonols and one flavanone. The structural identification was based on the mass, 1H and 13C NMR spectrometric analysis and two dimensional NMR, beyond comparison with literature data . The ethanolic extract of leaves of D. urucu was submitted to bioassays to avaluate the allelophatic potential, presenting relevant percentage of inhibition of seed germination and development of weeds in pastures. Aiming to detect the substances responsible for allelophatic activity, three stilbenes and three dihydroflavonols were selected and submitted to bioassays of inhibition of germination and development of the radicle and hypocotyl of weeds. The allelophatic tests were performed with the substances isolated and with the combination of them, aiming to assess the synergism between them, but the magnitude of inhibition observed was very low. By other side, when the substances were tested in mixtures there was a significant increase in the percentage of inhibition, so these substances in mixtures, can be considered promising for future studies involving allelophatic activity. An other test was carried out with the isolated compounds. Three stilbenes and five dihydroflavonols were evaluated for DPPH radical scavenging activity (antioxidant activity). In this bioassay, was not observed a significant antioxidant activity, justified by the analysis in the structures of the substances tested.Item Acesso aberto (Open Access) Obtenção e caracterização da raiz pulverizada da espécie entada polyphylla benth visando aplicação tecnológica em produto sapônico bioecológico(Universidade Federal do Pará, 2024-12-09) JESUS, Mário Lucivaldo Barreto de; BRASIL, Davi do Socorro Barros; http://lattes.cnpq.br/0931007460545219; HTTPS://ORCID.ORG/0000-0002-1461-7306; ALVES, Cláudio Nahum; http://lattes.cnpq.br/8315600067791313; https://orcid.org/0000-0001-6576-4229This study focuses on the physicochemical characterization of the pulverized roots of Entada polyphylla Benth, commonly known as gipioca, aiming at its application in the development of a bioecological soap product, the “vegetable soap.” This Amazonian species is rich in saponins, compounds with bioactive properties including antioxidant, surfactant, and emulsifying actions, as well as cytotoxic potential. Samples were collected from three locations in the municipality of Acará, Pará, identified at the Embrapa herbarium (NID 51/2023), and subjected to physicochemical and biological analyses. Results revealed 75.92% antioxidant compounds, 17.26% total saponins, 20.32% moisture, and 12.28% tannins, highlighting the plant's potential as a source of amphiphilic and bioactive compounds. Saponin extraction was performed via sustainable water maceration, avoiding the use of harmful chemical solvents. Laboratory tests demonstrated that the pulverized material generates significant foam upon contact with water, confirming its surfactant properties and suitability for cleaning applications. The developed product was patented (BR 102024016942 5) and presented at scientific events, such as the SBPC, where it was recognized for its innovation and alignment with circular economy principles. The study also assessed the biological activity of saponins through cytotoxicity assays on tumor cell lines (AGP01, A-549, SK-MEL 19) and a non-neoplastic cell line (MRC5). Results indicated selective cytotoxicity against tumor cells, emphasizing the therapeutic potential of the extract. In addition to promoting sustainable technological solutions, the study contributes to the valorization of regional biodiversity and offers socioeconomic benefits to riverside communities, including job creation and income generation through sustainable resource management. This research expands the understanding of Amazonian legumes’ chemistry, particularly the bioactive properties of saponins, and underscores their potential as substitutes for synthetic surfactants in industries such as cosmetics, therapeutic products, and cleaning agents. It is concluded that Entada polyphylla is a promising source of natural bioactives, with technological applications that integrate innovation, socioeconomic development, and environmental preservation.