Programa de Pós-Graduação em Farmacologia e Bioquímica - FARMABIO/ICB
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Item Acesso aberto (Open Access) Efeitos da Curcuma longa em modelo murino de acidente vascular cerebral(Universidade Federal do Pará, 2024-09) SANTOS, Vitória Corrêa; RÊGO, Dielly Catrina Favacho Lopes; http://lattes.cnpq.br/1810961422826950; https://orcid.org/0000-0002-6226-4269Stroke is the third leading cause of death and the main cause of functional impairment in adults. It can be hemorrhagic in nature, when a blood vessel in the brain ruptures, or ischemic, when there is obstruction of cerebral arterial blood flow. Ischemic stroke accounts for 87% of cases and is characterized by excitotoxicity, oxidative stress, neuroinflammation and cell death. Currently, treatment for ischemic stroke is limited to tissue plasminogen activator (tPA) therapy or mechanical thrombectomy, which makes the search for new pharmacological approaches crucial. In this scenario, Curcuma longa Linn (C. longa), known as turmeric, is a plant popularly used in cooking and traditional medicine and its main active compound is curcumin, responsible for giving C. longa anti-inflammatory, antioxidant, antimicrobial, antitumor, anticancer effects, among others. In the literature, C. longa has demonstrated promising activity against lesions caused by cerebral ischemia; however, the prolonged effects of the compound remain unknown. In this sense, this study evaluated the possible neuroprotective effects of C. longa in a murine model of transient focal cerebral ischemia. For that, 20 adult male Wistar rats (8 weeks old, weighing 300 ± 20 g; CEUA-UFPA no. 6868300622 [ID 001229]) underwent middle cerebral artery occlusion (MCAO) surgery for 30 minutes and treated with C. longa (MOTORE®) at a dose of 80 mg/kg every 12 hours for 14 days. The animals were divided into 4 groups (n = 4-5 animals per group): 1) Sham + V (animals with sham surgery that received vehicle [0.5 M NaOH + PBS]), 2) Sham + CL (animals with sham surgery that received C. longa), 3) MCAO + V (animals submitted to MCAO surgery that received vehicle) and 4) MCAO + CL (animals submitted to MCAO surgery that received C. longa). Metabolic parameters such as weight gain and water and food consumption were evaluated, as well as behavioral parameters through the neurological deficit score and the beam walking test, as well as histopathological parameters with the measurement of the infarct area and volume. In our results, no differences in body weight gain were found between the experimental groups. However, the sham + CL group consumed more water than the sham + V, MCAO + V and MCAO + CL groups, and the MCAO + CL group consumed less food on the 11th and 13th day after ischemia. Regarding behavioral deficits, both in the analysis of neurological deficit and in the beam walking test, the motor impairments evidenced by the MCAO + V and MCAO + CL groups were not attenuated by C.longa-treatment. Furthermore, treatment with C. longa did not attenuate the lesions caused by cerebral ischemia in our histological analyses. Thus, we conclude that treatment with Curcuma longa for 14 days did not exert a neuroprotective effect in the murine model of ischemic stroke, under our experimental conditions.Item Acesso aberto (Open Access) Evaluation of the acute toxicity and antioxidant activity of Justicia secunda methanolic extract in a murine sepsis model(Universidade Federal do Pará, 2024-09) DAVIS, Kelly; LIMA, Kely Campos Navegantes; http://lattes.cnpq.br/7998567135164137; HTTPS://ORCID.ORG/0000-0001-8613-3223; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; https://orcid.org/0000-0002-3328-5650Sepsis is a clinical condition that involves a dysregulated immune response to an infection, leading to complications such as organ dysfunction. The condition begins with hyperinflammation, followed by an increase in oxidative stress and depletion of antioxidant defenses. The rates of sepsis and septic shock have increased markedly in the last couple of decades. Therefore, the search for new therapies to aid in sepsis treatment is important. Justicia secunda Vahl is a widely used specie in traditional medicine. It has anti-inflammatory, antinociceptive, antioxidant, antianemic, antidiabetic, and antimicrobial properties due to secondary metabolites such as flavonoids, polyphenols, alkaloids, and terpenes. We evaluated a J. secunda methanolic extract (JSLS) in an acute oral toxicity test and as a pretreatment in a murine cecal ligation and perforation (CLP) sepsis model. In the acute oral toxicity test, we evaluated clinical parameters for 14 days after a single dose of 2000 mg JSLS/kg body weight, as well as oxidative stress parameter in organ samples. Subsequently, we evaluated the survival and antioxidant parameters of septic mice pretreated with 400 mg JSLS/kg body weight. In the acute oral toxicity test, there were no signs of toxicity, suggesting that JSLS has a median lethal dose greater than 2000 mg/kg body weight. JSLS pretreatment improved the survival rate, clinical parameters, antioxidant levels, and hematological profile of septic mice. We conclude that JSLS could be applied as a coadjutant agent to manage oxidative stress-related diseases. Additional studies are needed to better elucidate the mechanisms, as well as the effective dose of the chemical compounds present in JSLS.