Teses em Biologia de Agentes Infecciosos e Parasitários (Doutorado) - PPGBAIP/ICB

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/4698

O Doutorado em Biologia de Agentes Infecciosos e Parasitários teve início em 2005 e funciona no Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários (PPGBAIP) do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA).

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Acesso aberto (Open Access)
Caracterização molecular dos vírus do grupo Gamboa (Bunyaviridae, Orthobunyavirus) isolados nas américas e infecção experimental em pintos (Gallus gallus domesticus) com o vírus Gamboa cepa Be AN 439546
(Universidade Federal do Pará, 2010-03-31) CHIANG, Jannifer Oliveira; VASCONCELOS, Pedro Fernando da Costa; http://lattes.cnpq.br/0973550817356564
Presently, little information on Gamboa serogroup viruses (Bunyaviridae, Orthobunyavirus) is available. Thus, in this work, it was performed a comparative phylogenetic study on the members of the Gamboa serogroup and with other orthobunyaviruses to the level of the gene Gn (M-RNA); an experimental infections in the domestic bird (Gallus domesticus) using the strain Be AN 439546 of the Gamboa Virus (GAMV); and a serologic study using the Hemagglutination Inhibition (HI) test in serum samples of wild animals and humans collected in Tucuruí - Pará. The phylogenetic analysis of Gamboa group viruses demonstrated that they are genetically closely related to group Turlock viruses and less related to the Simbu group viruses. The group Gamboa viruses were distributed in two clades (I and II), that it is in agreement with the current serologic classification; the clade I correspond to the Gamboa complex and the clade II to the Alajuela complex. The strain Be AN 439546 presented tropism for chikens lung and liver, with viral replication in this organs confirmed by detection of viral antigens by immunohistochemistry. These results, demonstrate that this bird species is a susceptible host for GAMV replication. The detection of HI antibodies against GAMV, confirmed by neutralization tests were found in wild bird plasmas and reinforces the hypothesis that these animals constitute the main amplification hosts in the maintenance cycle of GAMV. Full length genome studies of the Gamboa serogroup viruses, as well as on the ecoepidemiology of their vectors and potential vertebrate hosts are needed to generate new data and to reinforce the understanding of the information already existent on those viruses.
Acesso aberto (Open Access)
Correlação dos níveis séricos e dos polimorfismos nos genes de citocinas (TNF-α, INF-γ, TGF-β1 e IL-10) com a apresentação clínica da hepatite B crônica
(Universidade Federal do Pará, 2010) CONDE, Simone Regina Souza da Silva; VALLINOTO, Antonio Carlos Rosário; http://lattes.cnpq.br/3099765198910740
Chronic hepatitis B has a wide spectrum of clinical manifestations resulting from various factors, such as the pattern of secretion and polymorphism in cytokine genes. This work aims to correlate the TNF-α -308G/A, INF-γ +874A/T, TGF-β1 -509C/T e IL-10 -1081A/G polymorphisms and serum levels of these cytokines with the clinical presentation of hepatitis B. It was selected 53 consecutive cases of hepatitis B divided into group A (inactive carrier= 30) and B (chronic hepatitis / cirrhosis= 23). As a control, we selected 100 individuals anti-HBc and anti-HBs positives. Serum levels of cytokines were determined by enzyme immunoassays (eBiosceince, Inc. California, San Diego, USA). The gene amplification of cytokines was carried out by PCR and histopathological analysis followed by METAVIR classification. It was identified that genotype TNF-α -308GA was more prevalent among B group than controls and that presence of A allele increased the risk for chronic disease (OR= 2,6). The serum levels of INF-γ and IL-10 were higher (p <0,001) in controls than others groups A and B and the TGF-β1 levels were lower (p < 0,01) in controls. It was noted that inflammatory activity > 2 correlated with higher levels of TNF-α and IFN-γ (p<0.01) and fibrosis > 2 with higher levels of INF-γ (p <0.01). In the studied population, lower INF-γ and IL-10 levels and higher TGF-β1 level were associated with chronic hepatitis B, and that the presence TNF-α -308 A allele increased in 2,6 the risk for chronic disease .

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Navegando Teses em Biologia de Agentes Infecciosos e Parasitários (Doutorado) - PPGBAIP/ICB por CNPq "CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS"