Teses em Biologia de Agentes Infecciosos e Parasitários (Doutorado) - PPGBAIP/ICB

URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/4698

O Doutorado em Biologia de Agentes Infecciosos e Parasitários teve início em 2005 e funciona no Programa de Pós-Graduação em Biologia de Agentes Infecciosos e Parasitários (PPGBAIP) do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA).

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Acesso aberto (Open Access)
Associação entre marcadores da resposta inflamatória e a imunopatogênese de agentes infecciosos de natureza viral (Vírus da dengue, HTLV-1 e HTLV-2) e bacteriana (Chlamydia trachomatis e Chlamydia pneumoniae)
(Universidade Federal do Pará, 2010-11-29) FEITOSA, Rosimar Neris Martins; ISHAK, Ricardo; http://lattes.cnpq.br/5621101706909450
The genetic basis of diseases is frequently studied aiming the polymorphisms of cytocine genes. The present study investigated markers of the inflammatory response associated to the course of infection and disease caused by viruses and bacteria. Serum levels (measured by an ELISA assay) and the polymorphisms (using PCR, RFLP and allele specific PCR) of TNF-α (-308), TNF-β (+252), IFN-γ (+874) and C reactive protein were measured among persons with febrile disease, infected by dengue virus (n=80), not infected by DV (100), a group of HTLV infected (30 symptomatic and 47 asymptomatic), a group with coronary disease (58 seroreactive to Chlamydia and 31 with negative serology) and a control group (99 persons with no reaction to DV, HTLV and Chlamydia). No group showed association with demographic informations. Dengue virus 3 (66.2%) and HTLV-1 (90% symptomatic and 76.6% asymptomatic persons) were the most frequent agents found among their groups. The majority of those with coronary disease (65.1%) presented antibodies to Chlamydia (39.6% to C. trachomatis and C. pneumoniae, 58.6% solely to C. trachomatis and 1.7% to C. pneumoniae). Statistically significant levels of differences were found among: (i) serum levels of TNF-β, IFN-γ and PrtCR of positive and negative dengue and control groups (p< 0,01); (ii) serum levels of TNF-α, TNF-β and IFN-γ of HTLV (including its types) and control groups; (iii) serum levels of TNF-α, TNF-β, IFN-γ and PrtCR among patients with coronary disease, serum reactive to Chlamydia, and the control group; (iv) the presence of antibodies to C. trachomatis and C. pneumoniae and the control group comparing TNF-β, IFN-γ and PrtCR. Genotypic frequency distributions were statistically significant for the polymorphisms: (i) of TNF-α (p=0,0494) and IFN-γ (p= 0,0008) genes among positive, negative and control dengue groups and to IFN-γ (p= 0,0007) among groups DEN 1, DEN 2, DEN 3 and controls; (ii) of IFN-γ gene (p= 0,0023) among the group of patients with coronary disease and sero reactivity to C. trachomatis e C. pneumoniae, as well as to the mono reactants in the comparison between the positivity to C. trachomatis and the control group.
Acesso aberto (Open Access)
Caracterização genética, correlação antigênica e ecoepidemiológica dos vírus do grupo C (Bunyaviridae, orthobunyavirus) isolados nas américas
(Universidade Federal do Pará, 2005-02-28) NUNES, Márcio Roberto Teixeira; ROSA, Amélia Paes de Andrade Travassos da; VASCONCELOS, Pedro Fernando da Costa; http://lattes.cnpq.br/0973550817356564
To date, no molecular studies on group C viruses (Bunyaviridae: Orthobunyavirus) have been published. The current work determined the complete small RNA segment and partial medium RNA segment nucleotide sequences for group C members. The full-length SRNA sequences ranged from 915 to 926 nucleotides in length, and revealed similar organization in comparison with other orthobunyaviruses. Based on the 705 nt of the N gene, group C members were distributed into 3 major phylogenetic groups, with the exception of Madrid virus that was placed outside of these 3 groups. Analysis of the Caraparu virus strain BeH 5546 revealed that it has an SRNA sequence nearly identical to that of Oriboca virus and is a natural reassortant virus. In addition, analysis of 345 nucleotides of the Gn gene for seven group C viruses and for strain BeH 5546 revealed a different phylogenetic topology, suggesting a reassortment pattern among them. These findings represent the first evidence for natural reassortment among the group C viruses, which include several human pathogens. Furthermore, our genetic data corroborate previous antigenic relationships determined using serologic assays (complement fixation, hemagglutination inhibition and neutralization tests), and suggest that a combination of informative molecular, serological and ecological data is a helpful tool to understand the molecular epidemiology of orthobunyavirus.
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Caracterização molecular e relação filogenética do genoma completo dos arbovírus Bussuquara, Iguape, Ilhéus e Rocio (Família Flaviviridae, Gênero Flavivirus)
(Universidade Federal do Pará, 2009-11-27) MEDEIROS, Daniele Barbosa de Almeida; NUNES, Márcio Roberto Teixeira; http://lattes.cnpq.br/0299116892743368; VASCONCELOS, Pedro Fernando da Costa; http://lattes.cnpq.br/0973550817356564
Flaviviruses have been known due their complex biological cycle and their relevance in public health and global economy. The ecologic aspected and clinic pictures are related with phylogeny and evolution of flaviviruses. This work aims the molecular characterization of Bussuquara (BSQV), Iguape (IGUV), Ilheus (VILH) e Rocio (VROC) flaviviruses genomes, determining their phylogenetic relationships with others members of genus Flavivirus. The study included: the full-length sequencing of the four Brazilian flaviviruses; analyzes of the predictive secondary structure of RNA and conserved sequences in the 3’NCR; determination of cleavage and glicosilation sites, cisteine residues and conserved motifs in the polyprotein; and similarity and phylogenetic analyzes. The BSQV, IGUV, VILH and VROC genomes present 10815 nt, 10922 nt, 10775 nt, and 10794 nt, respectively. The conserved standard sequences in 3’NCR of BSQV was RCS2-CS2-CS1, while to IGUV, ILHV and ROCV were CS3-RCS2-CS2-CS1. The secondary structure of RNA obtained for the Brazilian flaviviruses were similar to the other flaviviruses. The numbers of the glicosilation sites to PrM, E and NS1 proteins were distinct among the studied Brazilian flaviviruses, therefore the pattern 6,12,12 Cis residues and the cleavage sites were conserved. In the E protein, some singles mutations were observed in fusion peptide of BSQV, IGUV and ROCV, and the RGD motif were distinct for the flaviviruses under study. The motif that determines the MTase-SAM activity in NS5, as well as the helicase and protease activity in NS3 were conserved. Among the eight polimerase motifs in NS5, only the V, VI and VII motifs were observed single mutations in ILHV and ROCV. The similarity analyzes showed that BSQV presents high relationship with VIGU, while ILHV and ROV were more related among themselves, however those viruses were considerated distincts species. Based in the phylogenetic analyzes, molecular and biological characteristics, it was proposed the establishment of three distinct genetic groups: the Rocio group, grouping ILHV and ROCV, Bussuquara group formed by BSQV and Naranjal virus; and Aroa group, that include Aroa virus and IGUV.
Acesso aberto (Open Access)
Estudo epidemiológico de agentes virais (HIV, HTLV, VHB e CMV) identificados em adolescentes grávidas atendidas em um centro de referência do Sistema Único de Saúde de Belém, Pará
(Universidade Federal do Pará, 2010-11-05) GUERRA, Aubaneide Batista; MACHADO, Luiz Fernando Almeida; http://lattes.cnpq.br/8099461017092882
The prevalence of infection by viral agents such as HIV-1, HTLV1/2, VHB and CMV is not so much known in pregnant teenage population from the North region of Brazil. One of the objectives of this study was to describe the prevalence of infections HIV-1, HTLV1/2, VHB and CMV in pregnant adolescents, attended in a Reference Center in the State of Pará. To achieve the objectives, it was collected blood samples from 324 pregnant adolescents, who came from several cities of the State, from November of 2009 to February of 2010. The samples were submitted to an immuno-enzymatic test (ELISA), in order to detect antibodies Anti- HIV, Anti- HTL V1/2, Anti- VHB and Anti- CMV IgM/ IgG. The serological analysis revealed one serum positive sample for HIV-1, two positives samples for HTLV1/2, while the majority of samples showed antibodies Anti-CMV-IgG, although the occurrence of high infection was low (2.2%). The prevalence of infection for VHB in pregnant adolescents was 0.62%, however a great number (83.3%) of adolescents are susceptible to the infection by VHB, what means they probably were not immunized. The majority of adolescents (63.4%) continued their studies, even though they know about their pregnancy and 34.6% started prenatal later, so they only had a minimum number of four consultations of prenatal. The result reinforces the hypothesis that pregnant adolescents have prevalence for those viral type infections, what is similar to the national pregnant women rates. The prevalence of viral subtypes (HTLV-2 and HIV-1 subtype B) obtained by the molecular characterization of serum positive samples from pregnant adolescents agreed with the prevalence of subtypes from the North region.
Acesso aberto (Open Access)
Soroprevalência de anticorpos e padronização do teste ELISA sanduíche indireto para 19 tipos de arbovírus em herbívoros domésticos
(Universidade Federal do Pará, 2010-09-30) CASSEB, Alexandre do Rosário; VASCONCELOS, Pedro Fernando da Costa; http://lattes.cnpq.br/0973550817356564
The brazilian Amazon region maintains the largest variety of arboviruses and the state of Pará is responsible for 26% of this territory, so the major goal of this work was to determine the prevalence and distribution of HI antibodies to 19 domestic arbovirus in domestic herbivores in the state of Pará and to standardize an indirect sandwich IgG ELISA test to serum samples of equines, cattle, buffaloes and sheep. In all species studied and throughout the State of Pará a large prevalence of HI antibodies to all arbovirus analyzed was observed and SLEV, ILHV, EEEV, MAGV and WEEV, showed higher prevalence, where the SLEV was the most prevalent. Regarding the virus families HI antibodies to MAGV was the most prevalent Bunyaviridae in all species, the most prevalent Flaviviridae was SLEV in all species and in the family Togaviridae the EEEV was more prevalent in horses. In order to analyze the prevalence of HI antibodies by animal species was observed that horses did not show significant differences compared to buffaloes, however, showed significant difference compared to cattle and sheep; there was not observed significant difference between the ruminant species. Using sandwich indirect IgG ELISA a large number of crossed reactions were found. This enzymatic test can be used to detect IgG antibodies among families of arboviruses studied.

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Navegando Teses em Biologia de Agentes Infecciosos e Parasitários (Doutorado) - PPGBAIP/ICB por CNPq "CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::VIROLOGIA"