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Acesso aberto (Open Access)
Discrimination ability of Santa Inês and crossbred Santa Inês × Dorper lamb heavy carcasses by the Brazilian and European classification systems
(Universidade Federal do Pará, 2017-06) LIMA, Alyne Cristina Sodré; SOUSA, Marco Antonio Paula de; ARAÚJO, Jonas Carneiro; NUNES, Manuella Paula de Mesquita; MORAIS, Eziquiel de; ANDRADE, Stefano Juliano Tavares de; DAHER, Luciara Celi Chaves; SILVA, André Guimarães Maciel e
The ability of discriminating carcass characteristics of different fat cover scores of heavy carcasses, according to the European (EUS) and Brazilian (BRS) classification systems, was assessed. Fifty-six lambs, weighing between 26.25 and 46.15 kg, of the Santa Inês and crossbred Santa Inês × Dorper genetic groups were evaluated. The level of adiposity was assessed through color photography of the carcasses after refrigeration according to the EUS and BRS. The carcasses were assigned to four groups by cluster analysis according to 25 variables, namely, cold carcass weight; muscle; bone; fat; and muscle:fat and fat:bone ratios of the carcass and cuts (hindquarter, shoulder, back); kidney, pelvic, and inguinal fat; and subcutaneous fat thickness. Of the four groups obtained by cluster analysis, the scores according to the different classification systems only statistically differed between groups 1 and 4. The BRS had a higher number of variables well correlated with the scores by assessors than the groups classified by the EUS. The BRS was better correlated with tissue composition. However, most variables were better correlated with backfat thickness than the score obtained through the classification systems. Better results were obtained regarding the prediction of carcass fat by the BRS using backfat thickness or cold carcass weight. The Brazilian lamb carcass classification system better predicts tissue composition and is the best method to discriminate intermediate-fat classes when associated with cold carcass weight.
Acesso aberto (Open Access)
Efeito do meio condicionado por células tronco mesenquimais derivadas de tecido adiposo na maturação de oócitos bovinos e posterior desenvolvimento embrionário in vitro
(Universidade Federal do Pará, 2016-07-29) VALENTE, Juliana Vieitas; MIRANDA, Moysés dos Santos; http://lattes.cnpq.br/3354029928888919
Stem cells are known for their property of self-renewal, differentiation into various cell lineages and immunomodulatory capabilities, in addition to express a large number of adhesion molecules, extracellular matrix proteins, cytokines and growth factor recptors, allowing interactions with other cells. They can be isolated from various tissues, but adult stem cells derived from adipose tissue stroma (MSC-d) have the advantage of being easely isolated, high yield and low morbidity. Based on this, the study hypothesis is to verify the effect of MSC-ad conditioned medium use in in vitro maturation on development rates of bovine embryos. To this end, bovine MSC ad were isolated then cultivated until the passage three (P3) in DMEM culture medium, supplemented with sodium bicarbonate, 10% fetal bovine serum (FBS), 50mL / mL gentamicin. When cultures reached 70% confluency, the medium was replaced with TCM199 supplemented with 0.2 mM pyruvate, 50 ul / ml gentamicin and 10% FBS (IVM medium). The MSC-ad were conditioned with medium TCM199 supplemented for a period of 0 (Control Group), 24 (COND-24 group), 48 (COND-48h group) and 72 hours (COND group-72h), wherein the end of each period, the supernatant medium was removed, filtered, aliquoted and frozen at -20 0 C. The medium was thawed only on MIV day, and supplemented with 0.5 uL of PMSG (concentration 7 IU / ml) and 0.5 uL of HCG. Slaughterhouse ovaries were punctured to obtain oophurus cumulus complexes (COCs) were matured for 24 hours in microdrops of conditioned mediums under mineral oil them incubated in an incubator at 5% CO 2 at 38.5 ° C. Nuclear maturation assessment was performed with 22 hours, then held 15 parthenogenetic activation of only oocytes that exhibited polar body concentration of at least 50 uM ionomycin for 5 minutes, inactivation with TCM199-Hepes 0.03g saturated with BSA, followed by incubation for 3 hours microdroplets 2mM 6-DMAP. After this period, the parthenogenesis were transferred as microdrops to a culture medium in vitro. development rates were evaluated in the second and seventh days after activation. The results were analyzed by ANOVA and Fisher's post-test adopting a significance level of 5%. For groups: Experimental CONTROL, COND-24, COND-48h and 72h-COND, we obtained the following results respectively: 83.7, 77.7, 81.4 and 76.1% of nuclear maturation; 87.5, 86.9, 74 and 80.3 and 23.8% cleavage, 27.5, 18 and 19.6% of blastocyst formation. There was no statistical difference between condinioned experimental groups (p<0.05). However, blastocyst rates were lower when compared with fresh IVM medium (42.1%) (p<0.05). This suggests that the efficiency was affected by the conditioning of the medium or freezing. Future studies should be conducted to assess the levels of growth factors, cytokines and chemokines secreted in IVM medium.
Acesso aberto (Open Access)
Estabilidade aeróbia, pH e dinâmica de desenvolvimento de microrganismos da cana-de-açúcar in natura hidrolisada com cal virgem
(Universidade Federal do Pará, 2011-04) DOMINGUES, Felipe Nogueira; OLIVEIRA, Mauro Dal Secco de; SIQUEIRA, Gustavo Rezende; ROTH, Anna Paula de Toledo Piza; SANTOS, Juliana dos; MOTA, Diego Azevedo
The objective of this work was to evaluate the effect of whitewash addition and the times after application on aerobic stability and growth of microorganisms (fungi and yeasts) in fresh sugarcane. It was used a completely randomized experimental design, with four replications. Aerobic stability (temperature) and the concentrations of yeast and fungus and pH were evaluated in a split-splot scheme with five doses of whitewash (0; 0.5; 1.0; 1.5 and 2.0%) and five times after treatment (0, 24, 48, 72 and 96 hours). There was a significant effect of doses of whitewash for all evaluated parameters. The sugarcane which was not treated with whitewash (0.0%) showed a break in stability at the shortest time interval. The pH values increased gradually according to the dose of whitewash applied and they decreased over time. There was a numerical increase on the counting of yeast until 72 hours after application of whitewash, regardless to the dose, and the values tended to stabilize in 96 hours after treatment. There was no significant effect of whitewash doses on fungi development. The use of whitewash is effective in yeast growth control and it also promotes aerobic stability in fresh sugar cane.
Acesso aberto (Open Access)
A new species and new records of Oxysarcodexia Townsend (Diptera: Sarcophagidae) from Brazil
(Universidade Federal do Pará, 2017-12) CARVALHO-FILHO, Fernando da Silva; SOUSA, José Roberto Pereira de; ESPOSITO, Maria Cristina
Oxysarcodexia cocais sp. nov. from Brazil is described and illustrated based on male specimens collected in a babassu palm forest in the state of Maranhão. The remarkable elongated surstylus (as long as cercus) and U-shaped male sternite 5 distinguishes the new species from other species in the genus. In addition, Oxysarcodexia nitida Soares & Mello-Patiu, 2010 is recorded for the first time from Brazil, and Oxysarcodexia adunca Lopes, 1975 is a new record from the Brazilian Amazon
Acesso aberto (Open Access)
Óxido de cálcio e Lactobacillus buchneri NCIMB 40788 na ensilagem de cana-de-açúcar in natura ou queimada
(Universidade Federal do Pará, 2011-11) SIQUEIRA, Gustavo Rezende; SCHOCKEN-ITURRINO, Ruben Pablo; ROTH, Anna Paula de Toledo Piza; DOMINGUES, Felipe Nogueira; FERRAUDO, Antonio Sergio; REIS, Ricardo Andrade
The objective of this trial was to evaluate the effects of the inclusion of calcium oxide (CaO) and/or L. buchneri (LB) on the losses and chemical composition changes of in natura and burned sugar cane silages. Silage treatments were: in natura sugar cane without additive (IS), with Lactobacillus buchneri (ISLB), with calcium oxide (ISCaO) equivalent to 1% of natural matter, and the combination of LB and CaO (ISLBCaO). Experimental design was completely randomized in a factorial scheme (2 × 4) considering status (burned or not) and additives (LB, CaO and LB+CaO) with three replications per treatment. Dry matter variation was lower for in natura and burned silages treated with CaO; regardless of the LB presence, the mean difference was 5.85 percentage units. In sugar cane silages without additives, higher dry matter recoveries were observed when the plant was ensiled with in natura silage (63.5%) compared with burned silage (46.8%). However, when additives (CaO and LB) were used, no significant differences occurred between the in natura and burned silage treatments. The presence of calcium oxide was the factor that provided the greatest discrimination among the data. Calcium oxide is effective in reducing losses and chemical changes on the in natura and burned silages. The L. buchneri acts effectively on burned sugar cane silages, and the latter are more susceptible to losses than those of in natura sugar cane.
Acesso aberto (Open Access)
Processo ensino-aprendizagem na abordagem analítico-comportamental no contexto da agricultura familiar
(Universidade Federal do Pará, 2019-04-03) FERREIRA, Rafael Peniche; CARVALHO, Soraya Abreu de; http://lattes.cnpq.br/2542726626028987; DARNET, Laura Angélica Ferreira; http://lattes.cnpq.br/3450720474559096