Navegando por CNPq "CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::MICOLOGIA"

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Acesso aberto (Open Access)
Avaliação in vitro da atividade antifúngica da Malpighia glabra linn. em agentes da cromoblastomicose
(Universidade Federal do Pará, 2017-08-29) CRUZ, Naila Ferreira da; SILVA, Moises Batista da; http://lattes.cnpq.br/5525661855611118; SALGADO, Claudio Guedes; http://lattes.cnpq.br/2310734509396125
Chromoblastomycosis (CBM) is a mycosis by implantation, chronic, with a cosmopolitan distribution, caused by melanized fungi. After transcutaneous implantation, the propagules of the CBM agents present a unique cellular and morphological plasticity. The cellular differentiation results in the appearance of muriform cells. The treatment of this mycosis is a challenge due to the absence of a standard antifungal, resulting in several therapy methods (physical and pharmacological), used isolated or associated, with little clinical cure success. Amazon region has a vast biodiversity of vegetables that need to be better characterized as to their chemical composition and applicability in the treatment of different diseases. In this variety of plants, the fruit of Malpighia glabra Linn. (acerola) has a high content of vitamin C, also presenting vitamins of the complex B, A, anthocyanins and flavonoids, which stand out for having different biological and therapeutic actions already demonstrated both in vitro and in vivo. A major interest is currently focused on the biological activities of quercetin, belonging to the class of flavonoids, as it exerts multiple pharmacological activities, presenting unique biological properties that can improve mental and/or physical performance and reduce the risk of different infections. This study aims to evaluate the in vitro antifungal activity of M. glabra extract in conidia and muriform cells of Fonsecaea spp. The extract of M. glabra presented antifungal activity in both, conidia and muriform cells, of the evaluated strains. Much interest is now centered on biological activities of the flavonoid quercetin. In addition to the crude extract, the conidia of different strains were also sensitive to different dilutions of the extract. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were evaluated. The geometric mean of the quercetin MIC for the conidia was 4.75 μg/mL and the geometric mean of the CFM was 11.31 μg/mL. Further study is needed so that M. glabra or quercetin, isolated or associated with another isolated component of the extract, may be used for the treatment of CBM in the future.
Acesso aberto (Open Access)
Caracterização fenotípica de isolados de Cryptococcus gattii
(Universidade Federal do Pará, 2005-02-25) MAGALHÃES, Mioni Thieli Figueiredo; MAGALHÃES, Luiz Marconi Fortes; http://lattes.cnpq.br/6032076996211936; LAZÉRA, Márcia dos Santos; http://lattes.cnpq.br/7911244508417625
Cryptococcus neoformans was described as human pathogen in 1894. Since then, studies about fungal aspects as biology, taxonomy, ecology and epidemiology have been developed with great importance to medicine and the environment. The fungus was recently classified in two separated species, C. neoformans (serotypes A, D and AD) and C. gattii (serotypes B and C) each one with well defined genetic and morphological features as well as ecology and epidemiology aspects. C. neoformans occurs mainly in immunocompromised hosts, especially those infected by HIV. In the other hand, C. gattii infects mainly immunocompetents individuals to all lifetimes. The main factors determining the cryptococcosys pathogenesis have relations with the host defenses state, the size of particle and strain virulence, this last for four main features: the polyssacharide capsule, the melanin production, the mating types (MAT and MATa) and thermotolerance. The fungus has a transitory life cycle between haploid yeast form and dykariotic filamentous forms. The sexual cycles includes a mixture of genetic parental genes that form progenies containing the genetic characteristics of both. The asexual cycle occurs through a process know as haploid fruiting. The species are heterothallic with two mating types MAT and MATa. Phenotypic characterization studies, including the species identification, serotype and mating type have a great importance for conductions of profound studies because of the close relation between the ecological variants features and its development cycle and multiplication, especially in Brazilian regions North and Northeast where serotype B is the most prevalent cause of meningitis in ordinary hosts. This work used the CGB techniques for methabolic analysis (species identification), where 28 strains were positive, characterizing C. gattii and two were negative, characterizing C. neoformans. The serotyping results, make among agglutination test reveals 28 strains of serotype B and two of serotype A. The mating type was realized by PCR technique and all strains were MAT.
Acesso aberto (Open Access)
Estudo do Paracoccidioides brasiliensis no município de Buriticupu, Amazônia maranhense
(Universidade Federal do Pará, 2010-10-01) CALVET, Clélea de Oliveira; ISHAK, Ricardo; http://lattes.cnpq.br/5621101706909450
The objective of this study was to verify the presence of Paracoccidioides brasiliensis in the municipality of Buriticupu, located in the Amazon Maranhense. Intradermics tests were conducted with paracoccidioidine in 491 individuals, with 60 intradermics positive to the antigen. There was no statistically significant difference in the sample in relation to the positive test and the variables gender, socio culturals habits and level of education, although describe, the female has been the most frequent, totaling 33 patients, and that positive - reagents have habits that provide contact with the pathogen, and it was found to be the prevailing levels of schooling illiteracy (18 tested) and 1 st degree incomplete (26 tested). Fifty-one patients testing positive for intradermics to paracoccidoidine (85%) were the natural state of Maranhão. In reviewing the clinical data and laboratory records in general of the six patients diagnosed with the disease paracoccidioidomycosis, found that all were farmers with more than 10 years of residence in Buriticupu, with the presence of the fungus in the oral mucosa scraped the colouring of PAS and Gomori - Groccot, except for two patients: one with 36 months of treatment and another with 12 months of therapy, and without injury initial oral or later, both without reports of abandonment of treatment. We, therefore, that the exfoliative cytology is an easy way and low cost in aid of therapeutic control of patients.
Acesso aberto (Open Access)
Imunoexpressão para CD1a em lesões cutâneas na doença de Jorge Lobo
(Universidade Federal do Pará, 2009) UNGER, Deborah Aben-Athar; BRITO, Arival Cardoso de; http://lattes.cnpq.br/0563291980190339
Jorge Lobo’s disease (DJL) is a chronic infection caused by the fungus Lacazia loboi, endemic in South America, especially in the Brazilian Amazon region. The most common clinical presentation is parakeloidal lesion located mainly in the lower limbs in men who practice agricultural activity. The fungus can be identified by both mycological and anatomopathological examination. The main objective of this study was to investigate the possible role of Langerhans' cells (LC) in the pathogenesis of the disease in tissue samples from skin lesions, using immunohistochemical technique. Thirty-three medical records were selected with their respective paraffin blocks of skin biopsies from patients with DJL (group 1) registered in the dermatology service at the Federal University of Para in the period from 1955 to 2005. The control group consisted of 10 paraffin blocks from normal skin (group 2) and 42 blocks from patients with paracoccidioidomycosis (PCM) (Group 3). In the analysis of patient medical records data were collected regarding age, sex, origin, profession, location and clinical type of the lesions. Langerhans cells were identified by immunohistochemistry using anti-CD1a antibody (Serotec). Patients were mostly men (84, 8%), farmers (72. 7%) aged between 46-65 years, with predominance of parakeloidal lesions (81. 8%) in the lower limbs (45. 5%). The number of positive cells was statistically analyzed. The LC were visualized along the epidermis in all biopsies from Jorge Lobo's disease. The morphology and the number of cells, did not differ from normal skin (p> 0. 05), and was increased when compared to the PCM lesions (p <0. 05). Langerhans cells were present in Jorge Lobo’s disease skin lesions similarly to the way they are in normal skin, not suffering any numerical or morphological alterations, unlike what occurred in PCM. These results suggest that in DJL fungi probably present some escape mechanism of the local immune system to evade the antigen presentation by Langerhans’ cells.
Acesso aberto (Open Access)
Influência dos metabólitos secundários de Piper divaricatum da região amazônica no controle do Fusarium solani f. sp. piperis causador da fusariose em pimenta do reino
(Universidade Federal do Pará, 2014-09-18) MEIRELES, Erisléia das Neves; RAMOS, Alessandra de Rezende; http://lattes.cnpq.br/1279694874191138; SILVA, Joyce Kelly do Rosário da; http://lattes.cnpq.br/2278686174214080
The culture of black pepper (Piper nigrum L.) is one of the main agricultural activities in Pará State and it suffers serious damages caused by fusariosis, disease restrict in Brazil. This paper evaluated the antifungal activity in vitro of essential oils of species of Piper rich in phenylpropanoid before the Fusarium solani f. sp. piperis, which causes the fusariosis. The inhibition of mycelial growth by the method of agar dissemination in concentration of 5 mg.mL-1 was considered: decrease to P. aduncum (20.3%) and P. Krukoffii (31.4%); moderated to P. callosum (55.7%) and P. marginatum (70.3%) and high to P. divaricatum (93.3%). The major components identified by CG-EM were dilapiol (92.0%), safrole (78.0%), methyleugenol (75.2%) and eugenol (7.9%), apiol (80.0%), Z-isoosmorizol (44.0%) and E-anethole (22.0%), respectively. The oil of P. divaricatum and its major compounds present CIM values of 0.75 mg.mL-1. The evaluation of the effects combined of eugenol and methyl eugenol pointed eugenol as the main responsible by the activity.
Acesso aberto (Open Access)
Inquérito com paracoccidiodina em cinco cidades do Estado do Acre
(Universidade Federal do Pará, 2011-09-30) FIGUEIREDO, Mediã Barbosa; ISHAK, Ricardo; http://lattes.cnpq.br/5621101706909450
Paracoccidioidomycosis constitutes an important public health problem in Latin America. In Brazil, this disease is less frequent in North and Northeast regions, predominating the endemic areas in the Southeast. Despite the physiographic conditions of the North region favoring the development of Paracoccidioides brasiliensis, even so, the case reports are scarce. Perhaps this is due to the little consideration of deep mycoses about the differential diagnosis of chronic medical conditions, justified by the fact that the region is not considered endemic. With the objective of describing the prevalence of paracoccidioidomycosis in five cities of Acre, a cross-sectional population-based study was conducted, through a survey with the paracoccidioidin intradermal. The reading of the intradermal test was performed within 24 and 48 hours, being considered reactors the people with hardened areas of 5mm or more in diameter. Four hundred thirty-nine (439) people were studied and paracoccidiodomycosis-infection in the 48-hour reading was 41.2%. The findings of this study indicate that P. brasiliensis infection causes a considerable number of people in the study area to get infected, and therefore future clinical-epidemiological and mycological studies should better clarify the natural history of mycosis in this region.