Programa de Pós-Graduação em Oncologia e Ciências Médicas - PPGOCM/NPO
URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/4631
O Programa de Pós-Graduação em Oncologia e Ciências Médicas (PPGOCM) integra o Núcleo de Pesquisas em Oncologia (NPO) da Universidade Federal do Pará (UFPA). Trata-se do único centro de referência em pesquisa e formação de recursos humanos stricto sensu na área de oncologia na região Norte do Brasil. Os outros centros se concentram nas cidades do Rio de Janeiro e São Paulo.
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Item Acesso aberto (Open Access) Dispositivo fisioterapêutico gerador de pressão positiva expiratória com propriedades fluxo-dependentes(Universidade Federal do Pará, 2016-12-21) NINA, Janize Costa; NORMANDO, Valéria Marques Ferreira; http://lattes.cnpq.br/7098261432975265; SILVA, Luiz Carlos Santana da; http://lattes.cnpq.br/6161491684526382Positive expiratory pressure therapy is safe and effective for the prevention, reversal of atelectasis and removal of pulmonary secretions. The experimental study aimed to elaborate a physiotherapeutic device capable of generating positive expiratory pressure for patients in spontaneous breathing and evaluating their mechanical performance. The composition of the physiotherapeutic device consisted of 14 components, produced in aluminum and plastic, allowing to present characteristics of a flow-dependent non-gravitational resistor. The pressures obtained through the high flux flowmeter model Certifir® FA TSI (TSI Corporated, USA) had influence of the orifice strength with diameters of 1.5, 2.0, 3.0, 4.0 and 5.0mm, five-piston weights (1.5; 1.6, 2.0, 3.2 and 3.8g) and constant flows of 3, 5, 6, 9, 10 and 12L/min or piston firing flow. Among the five pistons, the piston 4 (3.2g) presented a better statistically significant result, reaching a pressure of 20cmH2O with flows of 8.16L/min, for the 1.5mm diameter bore. The proposed PEP device can generate therapeutic pressures between 10 and 20cmH2O, through a variation of low expiratory flows. It presents as singular characteristic the association of the diameter of the orifice with the weight of the piston to generate the positive pressure. Future studies are needed in order to promote the validation of the physiotherapeutic device in healthy children and subsequent analysis in patients with pulmonary conditions to obtain scientific data that represent our clinical practice.Item Acesso aberto (Open Access) Efeito da suplementação de altas doses de colecalciferol sobre o comportamento da pressão arterial em pacientes normotensos com diabetes mellitus tipo 1(Universidade Federal do Pará, 2018-12-26) QUEIROZ, Natércia Neves Marques de; FELÍCIO, João Soares; http://lattes.cnpq.br/8482132737976863Type 1 diabetes mellitus is an endocrine disease highly associated to cardiovascular (CV) risk. Vitamin D (VD) deficiency has been associated to a burden of chronic diseases due to the presence of vitamin D receptors (VDR) through diverse human tissues such as smooth vascular muscle, endothelium, cardiomiocytes and juxtaglomerular cells. Some studies have suggested an inverse relationship between vitamin D levels and blood pressure. High mean blood pressure levels have been found in vitamin D deficient patients. Additionally, previous studies have suggested that the VD-VDR complex might act as a negative regulator factor over renin angiotensin system, which could be responsible for positive effects on blood pressure. The main objective of this study was to evaluate high doses vitamin D supplementation effects on blood pressure of normotensive T1DM patients. Our study was a prospective interventionist study in 35 T1DM patients. The patients with vitamin D lower them 30ng/ml received 10.000UI/daily e if was 30-60 ng/ml was gave 4.000UI/daily. They made 24-hour ambulatory blood pressure monitoring, gycated hemoglobin, creatin, lipids profile, PCRus, before and after 12 weeks. We found an expressing reduce of systolic and diastolic morning blood pressures (117 ± 14 vs 112±14, p<0,05; 74±9 vs 70±10 mmHg, p<0,05, respectively) with no changes in order pressoric markers. Besides, we notice correlation between levels of VD after supplementation and diastolic morning blood pressure (r= -0,4; p<0.05). In conclusion, our study suggest that was an association with supplementation of high doses of vitamin D and reduce of morning blood pressure in normotensives type 1 diabetes mellitus patients.Item Acesso aberto (Open Access) Efeito da suplementação de altas doses de vitamina D sobre a neuropatia autonômica cardiovascular em pacientes com diabetes mellitus tipo 1(Universidade Federal do Pará, 2018-12-10) SILVA, Lilian de Souza d’Albuquerque; FELÍCIO, João Soares; http://lattes.cnpq.br/8482132737976863; YAMADA, Elizabeth Sumi; http://lattes.cnpq.br/7240314827308306Cardiovascular autonomic neuropathy (CAN), associated with diabetes mellitus (DM), despite being a subclinical condition, is an important morbidity and mortality factor in these patients. In type 1 diabetes mellitus (DM1) CAN is so alarming that it must be screened after the first 5 years of illness. Few therapeutic measures are recommended in international guidelines on the subject. Some authors have been studying drugs that can modified natural history of disease and then improve outcomes. Vitamin D seems promising resource and low cost. The aim of our study was to evaluate the effects of high-dose vitamin D (DV) supplementation on CAN in patients with DM1. We performed a prospective, interventional study in which 17 patients diagnosed with DM1 and CAN were included. Patients with VD levels below 30 ng / mL received 10,000 IU / day, and when 30-60 ng / mL, they used 4,000 IU / day. Serum VD dosage and CAN tests were performed before and after 12 weeks of treatment. There was an improvement in the parameters related to resting heart rate (HR) variability, which were: LF (1.9 ± 0.4 vs 2.2 ± 0.7 sec, p = 0.05), TP (2.5 ± 0.3 vs 2.7 ± 0.5 sec, p <0.05) , RRmax (0.8 ± 0.09 vs 0.9 ± 0.23 sec, p <0.05), RRNN (0.72 ± 0.09 vs 0.76 ± 0.09 sec, p <0.05) and SDNN (0.015 ± 0.005 vs 0.026 ± 0.018 sec; p <0.05). In addition, it was demonstrated that the variation of the RV level correlated with both the final HF (after treatment) and the LF / HF ratio (r = 0.57, p <0.05). Our pilot study is the first to suggest a strong association between high-dose vitamin D supplementation and improved cardiovascular autonomic neuropathy in DM1 patients. This occurred without any variation in HbA1C, blood pressure levels, lipids and insulin doses usedItem Acesso aberto (Open Access) Estudo de polimorfismos no gene GRIK2 em pacientes com doença de Parkinson(Universidade Federal do Pará, 2017-01-18) BARBOSA, Suane Reis; SILVA, Luiz Carlos Santana da; http://lattes.cnpq.br/6161491684526382Parkinson's Disease (PD) is a complex neurodegenerative disorder resulting from the multiple combination of genetic and environmental factors. One of the factors that may contribute to PD development is the excitotoxicity, a pathophysiological process caused by intense stimulation of glutamatergic receptors. This neurotoxic phenomenon is associated with the excessive influx of ions in the cell (Na +, Cl- and especially Ca 2+), resulting in neuronal death. It was evidenced that the GluK2 subunit of the kainate type glutamate receptor interacts with parkin, accentuating the excitotoxic process. The GRIK2 gene encodes this subunit, expressed in regions of the brain involved in motor activity, and may undergo alternative splicing or RNA editing, introducing new isoforms that may alter the ion conductance at the receptor. There are no studies in the literature on the association of polymorphisms in the GRIK2 gene with PD. This study aimed to determine the genotypic and allelic frequencies, as well as to verify a possible influence of the SNPs rs3213607, rs2227281, rs2227283, rs2235076, rs4839797, rs2518261 from GRIK2 gene in a group of patients with PD. A case-control study was performed, with analysis of DNA samples from 129 individuals from the control group and 61 patients from the PD group. It was found that for the SNP rs2518261 (C/T), allele T appeared to have a risk effect in the DP group (x2= 19.085; p-value <0.0001; OR = 2.75; CI = 1.75-4 , 27). In this polymorphism it was also observed that TT genotype may represent a factor associated with the tremor presence in the PD group (p-value = 0.02). These pioneer results of this study, suggest that further research is needed to investigate the contribution of GRIK2 gene to PD.Item Acesso aberto (Open Access) Influência da suplementação de altas doses de vitamina D no controle glicêmico em pacientes com diabetes mellitus tipo 1(Universidade Federal do Pará, 2018-12-10) MELO, Franciane Trindade Cunha de; FELÍCIO, João Soares; http://lattes.cnpq.br/8482132737976863; YAMADA, Elizabeth Sumi; http://lattes.cnpq.br/7240314827308306Although the intensive glycemic control of Diabetes Mellitus (DM) with insulin has reduced the incidence of microvascular and macrovascular complications, most patients still develop these injuries with high morbidity and mortality. It has been suggested that low levels of vitamin D (VD) may be associated with the development of Type 1 diabetes mellitus (DM1) and poor glycemic control. As a therapeutic potential, the use of VD in patients with DM1 has presented controversial results regarding the reduction of glucose levels. The objective of this study is to analyze the effects of high-dose vitamin D supplementation on glycemic control of patients with DM1, assessed through glycated hemoglobin levels (HbA1c). A prospective, 12week clinical trial including 52 patients with DM1, which were supplemented with high doses of cholecalciferol, was performed. The dose used for this vitamin was according to the participant's VD value. Patients with VD levels below 30 ng / mL received 10,000 IU / day, and when 30-60 ng / mL, they used 4,000 IU / day. The levels of VD and HbA1c were evaluated before and after 3 months of vitamin supplementation. When we analyzed the total number of patients (N = 52), there was no improvement in the glycemic control evaluated by HbA1c ((9.3 ± 2.3 vs 9.5 ± 2.4, p=NS). To better study the effects of VD on HbA1c, patients were divided into 3 groups according to HbA1c variation: those whose HbA1c reduced ≥ 0.5% (group 1, N = 14); those with no variation in HbA1c (group 2, N = 19) and those with ≥ 0.5% increase in HbA1c (group 3, N = 24). There was a decrease in HbA1c in only one specific group (N = 14). In addition, there was no reduction in prandial basal insulin needs or full dose after three months of VD supplementation. Thus, our data suggest that there is no additional benefit of VD supplementation in the optimization of glycemic control evaluated by HbA1C in patients with DM1.Item Acesso aberto (Open Access) Modelo murino do espectro autista empregando o ácido valproico durante a gravidez: mudanças comportamentais e citocinas pró-inflamatórias(Universidade Federal do Pará, 2018-10-31) SOUZA, Dilza Nazaré Colares de; DINIZ, Cristovam Wanderley Picanço; http://lattes.cnpq.br/2014918752636286; DINIZ, Daniel Guerreiro; http://lattes.cnpq.br/3269424921125406The present study evaluated, in behavioral tests, the exploratory and locomotor activities of young adult BALB/c mice that were exposed to valproic acid during pregnancy and measured their proinflammatory cytokines concentration in peripheral blood. To this end, we exposure females to males and on day 12.5 of gestation, females received 0.2 mL of VPA diluted in saline (600 mg / kg body weight) or equal volume of saline solution. The pups were weaned on the 21st postnatal day and the males were kept either in the impoverished environment of standard laboratory cages (IE) or in an enriched cage (EE). Four independent experimental groups according to experimental condition and environment (Ctrl / EE, Ctrl / IE, VPA / EE, VPA / IE) were organized. At 5 months of age, all animals were submitted to Open Field (OF), Episodic Memory (EM), Burrowing and Elevated Plus Maze (EPM) tests and had their peripheral blood collected and then perfused with saline followed by aldehyde fixatives. Two-way ANOVA revealed significant influences of experimental condition (VPA vs. Saline) and environment (EE vs IE) on behavioral outcomes and on proinflammatory cytokines peripheral concentrations. In the open field, the valproic acid groups, regardless of the environment where they were kept, reversed the natural tendency to avoid the center of the arena, F (1,54) = 5.59, p = 0.022. Similarly, in the elevated plus maze, the valproic acid groups, independent of the environment where the animals were kept, showed a significant influence on the time spent in the central platform, reducing it significantly, F (1,51) = 7.57, p = 0.0082. Two-way ANOVA also demonstrated a significant influence of the experimental condition (VPA vs. Saline) on the immune response reducing IL-1β, F (1.49) = 26.24, p <0.0001 and increasing IL-6, F (1.46) = 16.96, p = 0.0002 of the valproic acid groups. BALB/c mice exposed to valproic acid during pregnancy shows significant changes on their behavior to explore novel environments and to assess risk at adulthood, and this is associated with proinflammatory cytokines peripheral changes. Somatosensory and cognitive stimulation of environmental enrichment seems to be not enough to reverse it.