Programa de Pós-Graduação em Biotecnologia - PPGBIOTEC/ICB

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/6089

O Programa de Pós-Graduação em Biotecnologia (PPGBIOTEC) é um programa do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA). O PPGBIOTEC fornece a base teórica para o desenvolvimento de projetos em duas áreas de concentração e quatro linhas de pesquisa, definidas de acordo com a competência e experiência de orientação dos docentes do programa: Área de Bioprospecção: linhas de pesquisa: (i) química orgânica de biomoléculas e metabolômica; e (ii) genômica, transcriptômica, proteômica e bioinformática de microrganismos e plantas (Bactéria, Archaea e Eukarya). Área de Biotecnologia Aplicada: linhas de pesquisa: (i) saúde humana e veterinária; e (ii) produção de insumos e processos industriais.

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Agora exibindo 1 - 3 de 3

Acesso aberto (Open Access)
Análise proteômica de cultura macrófagica tratada com ácido kójico
(Universidade Federal do Pará, 2023-07) ALMEIDA, Heyder Coutinho; SANTOS, Agenor Valadares; http://lattes.cnpq.br/9530734927662735; https://orcid.org/0000-0002-2690-2841
Kojic acid (AK) is a natural fungal secondary metabolite, produced by some species of Aspergillus, Penicillium and Acetobacter, through the direct biotransformation of carbon substrates such as glucose, sucralose, ethanol, among others. AK was originally isolated in Japan, in 1907, from Aspergillus oryzae mycelia, it is used in the cosmetic industry in the form of gels and soap and is used to obtain the effect of whitening the skin by inhibiting the enzyme tyrosinase, reducing the production of formation of melanin, it also blocks the formation of pigmentation and evens out the skin tone. It is also applied as a food additive for the prevention of enzymatic browning, as it is a metal chelator and a potent antioxidant. Although AK has numerous biological functions, only recently studies on in vitro proliferation and cytotoxicity have been reported, but still limitedly regarding its effect on immune cells. Macrophages are among the most important defense cells that specifically recognize and respond to foreign bodies, apoptosis cells and pathogens. Through the activation process, there is an increased proliferation of macrophages, which undergo various morphological changes, such as an increase in dissemination and adhesion abilities, phagocytosis activity, ROS generation, antigen presentation and cytokine production. According to the classification of biological functions, our results revealed that in both Kojic acid concentrations, the 14 expressed proteins have a predicted function related to cell cycle and redox processes.
Acesso aberto (Open Access)
Bioprospecção de leveduras com potencial para a produção de carotenoides presentes em uma microrregião do bioma Amazônia
(Universidade Federal do Pará, 2024-11) LUCAS, David Cristian Rodrigues; CHISTÉ, Renan Campos; http://lattes.cnpq.br/0583058299891937; https://orcid.org/0000-0002-4549-3297
Carotenoids are bioactive compounds used as natural pigments and are found in plants, animals, algae and microorganisms. Some of these compounds are precursors of vitamin A and have benefits to human health such as strengthening the immune system and reducing the risk of chronic degenerative diseases. In industry, the production of carotenoids is obtained mainly by chemical synthesis or through algae and plant extracts. However, with the growing concern about utilization of chemical additives in food by many consumers, the interest in obtaining them through biotechnological processes and diverse microorganisms may synthesize them, such as yeasts. In this work, an exploration study was carried out focused on molecular identification of carotenoid-producing yeasts available in a microregion of Amazonia biome, with the aim of highlighting promising microorganisms for future industrial applications. Samples were collected from leaves, flowers, soil, and bark trees, among them, 4 promising strains were isolated and identified from phyllosphere of philodendron (Philodendron hederaceum): Rhodosporidiobolus ruineniae (PH-18(I)), Rhynchogastrema noutii or Hannaella pagnoccae (dPH-8(I)), Leucosporidium egoroviorum or Lyomyces sp. (dPH-8(II)), and Rhodotorula diobovata or Rhodotorula glutinis (PH-22(II)). The strain PH-18(I) showed a biomass yield of 0.26% to 0.76% and total carotenoid content of 139.70 μg/g of dry biomass. The strain dPH-8(I) showed a biomass yield of 0.43% to 0.96 % and total carotenoid content of 54.26 μg/g. The strain dPH-8(II) demonstrated a biomass yield of 0.35% to 1.12% and total carotenoid content of 52.71 μg/g, while the strain PH-20(II) demonstrated a biomass yield of 11.63 % to 23.67 % and total carotenoid content of 44.98 μg/g. The carotenoids identified in this study were 13Z-β-carotene, (all-E)-β-carotene and 9Z-β-carotene, being (all-E)-β-carotene the majority. Results found in this study highlight a promising perspective for future applications of carotenoid-producing yeasts in the Amazonia biome, being an excellent alternative to replace the process of obtaining carotenoids by synthetic means or by exploitation of vegetal sources.
Acesso aberto (Open Access)
Caracterização molecular in silico de uma mono-oxigenase lítica de polissacarídeo cianobacteriana
(Universidade Federal do Pará, 2024-03) VIRGOLINO, Rodrigo Rodrigues; GONÇALVES, Evonnildo Costa; http://lattes.cnpq.br/8652560763793265; https://orcid.org/0000-0003-2221-1995 País de Nacionalidade Brasil
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of β(1-4) glycosidic bonds and have attracted great attention due to importance in increasing the efficiency in degradation of recalcitrant polymeric substrates, in synergism with the action of hydrolytic enzymes, as an accessory function. However, LPMOs act via oxidative cleavage rather than hydrolysis. In industrial applications, LPMOs of fungal origin are the most frequent, while other taxonomic groups have been described as possible alternative sources of these enzymes. In the present study, we aimed to identify and characterize in silico a LPMO of cyanobacterial origin with putative functions in chitin depolymerization. The search for sequence similarity and conservation of domains with other characterized LPMOs identified a 289 AA protein from the cyanobacterium Mastigocoleus testarum of the Nostocales Order, being a probable LPMO of the CAZy-AA10 class. This protein is referred to as MtLPMO10. Phylogenetic analysis revealed that MtLPMO10 is homologous to the Tma12 protein from the fern Tectaria macrodonta, with 52.11% identity, which was the first LPMO characterized as originating from the plant kingdom. The occurrence of shared structural and functional patterns with other AA10 class LPMOs, as well as the existence of variations in these established patterns, contributed to the understanding of the biological functions of this class of enzymes. The predicted protein tertiary arrangement by the AlphaFold server pointed out structural features common to LPMOs, especially a histidine brace composed of His31 and His132 and an immunoglobulin-like domain consisting of antiparallel beta strands. Molecular dynamics simulation (MD) allowed evaluating the enzyme-substrate affinity, using an initial pose based on data obtained from the literature. There was stability of the MtLPMO10-chitoheptaose complex during 100ns of MD, while the MtLPMO10-celloheptaose complex broke apart in 30ns of MD. Also, there was a shorter Cu(I)-H4 distance in the first complex compared to the Cu(I)-H1 distance (averages 6.0 ± 0.7 Å and 7.9 ± 0.7 Å, respectively), suggesting a C4-type regioselectivity, as defined for Tma12. This study highlights the existence of lytic polysaccharide monooxygenases in cyanobacteria and paves the way for further investigations related to this enigmatic class of enzymes and their potential use in biotechnological applications.

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