Universidade Estadual de Campinas - UNICAMP

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/8436

Navegar

Agora exibindo 1 - 6 de 6

Acesso aberto (Open Access)
Aspectos bioquímicos e morfológicos da matriz extracelular das cartilagens septal e alares do nariz suíno
(Universidade Estadual de Campinas, 1999-07-02) MESSIAS JÚNIOR, Nazário de Souza; GOMES, Laurecir; http://lattes.cnpq.br/0032653238117133
The objective of the present study was to characterize some aspects of the extracellular matrix components of the alar and septal cartilage of the nose of swine and to examine the histological and histochemical aspects of the two types of cartilage. The kaotropic agent Gu-HCl was used to extract the matrix components. The predominant glycosaminoglycan in the two caitilages was chondroitin sulfate. After ultracentrifugation, the D4 fraction of the septal and alar cartilages was fractioned on DEAE-Sephacel using na NaCl gradient. SDS-PAGE analysiys showed similarities between the two cartilages, with the presence of fíbromodulin, decorin and binding proteins. Only the septal cartilage presented the 115 kDa component, which possibly appears as 30 kDa subunits after the action of the reducing agent. The glycosaminoglycan populations were determined by fractionation on Sepharose CL-6B followed by analysis of the samples on the agarosepolyacrilamide gel. The two cartilages presented a strongly polydispersed population, which was less uniformly polydispersed in the septal than in the alar cartilage. AD2 fractionation on DEAE-Sephacel and sample analysis by SDS-PAGE and agarosepolyacrylamide electrophoresis showed the presence of the small proteoglycans biglycan and decorin, possible associated with the large proteoglycans. The septal cartilage presents chondrocytes roughly arranged in rows accompanyng the widest axis of the cartilage, whereas the alar cartilage presents chondrocytes characteristically arranged in isogenic groups. Histhochemistry was performed usisng Xylidine Ponceau for total proteins, Picrosirius-Hematoxylin for collagen, Toluidine Blue for total glycosaminoglycans. And alcian blues, pH 2.5 and pH 1.0, for sulfated and carboxylated glycosaminoglycans. Total glycosaminoglycans are more cleary visible in the territorial matrix, whereas total proteins are more cleary visible in the interterritorial matrix. The collagen of the septal cartilage is roughly organized along the longitudinal axis of the specimens between the chondrocytes rows.
Acesso aberto (Open Access)
Estrutura e ultraestrutura do epitélio epididimário de búfalos (Bubalus bubalis) em diferentes idades
(Universidade Estadual de Campinas, 1995-02-17) SANTOS, Jeannie Nascimento dos; DOLDER, Mary Anne Heidi; http://lattes.cnpq.br/7046643907575335
The zonation of the buffalo epididymis of Mediterranea (Me), Murrah (mu) breeds and croosbreed Me x Mu was studied in 4 to 36 month old animals. These buffalos were c1assified in two groups, the animals which have not reached puberty (sexually immature) and animals (mature animals) and their epididymis was divided in 7 subregions called A to G. each subregion was analysed with and transmission electron microscopy. Light microscopy inc1uded routine staining with Hematoxilin & Eosin (HE), histochemistry with P AS and morphometric analysis for tubular and luminal diameters and height of epithelium and stereocilia. Throughout the epididymary tubule it was observed with light microscopy that tubules of alI ages have Principal cell (P), basal cell (B), Apical ceeII (A) and intraepithelialleukocytes (LI). Mitotic figures in the apical region of the epithelium are also found and are more numerous in the B and C subregions, specially in buffalos between 5 and 9 months of age. Histochemical analysis revealed the existence of secretory activity from the 5th month of age, principally in the A subregion, where the activity increases with sexual maturation. Morphometric analysis supports the idea that the buffalos which have not reached puberty already havee weell determined diameters and height variations of the tubule, lumen, epithelium and stereocilia. These variations were similar to that observed in buffalos after puberty. Tubular and luminal diameters increase from the A to G subregions while the height of epithelium and stereocilia diminish. However, in both morphometric features a significant difference (p
Acesso aberto (Open Access)
Fauna associada as bromelias Cannistran aff. giganteum (Baker) L. B. Smith e Neoregelia cruenta (R. Graham) L. B. Smith de restinga do litoral norte do Estado de São Paulo
(Universidade Estadual de Campinas, 1981) FERREIRA, Clara Pantoja; BENSON, Woodruff Whitman; http://lattes.cnpq.br/7116492347323901
An experimental set up for the study of tablets coating in a two-dimensional spouted bed that uses vibration of the perforated base to enhance the circulation of the particles was developed. An acrylic rectangular upper section (4x32 cm) with fixed 4x4 cm draft plates was adjusted over 3 different convergent secctions (inclination 50') with 4x4 cm, 4x6 cm or 4x8 cm bases and contained 800g, 1100g or 1300g of tablets, Air (0,8 to 1,6 m3/min) forced by a 7,5 cv blower, was cooled down to 55°C and spouted this bed. The lenticular tablets (0,12g; 0,25g., 0,44g) were coated with a polymeric aqucous solution (11% solids). A two-fiuid atomizer (air pressure 82,7 1(.Pa) was used, inserted lateraily, near the bed base, and showed no influence on the movement of the particles. The experimental characterization of the tablets circulation rates was emphasized, using a magnet (0,4062 g) that followed the particles, activating a bobbin located at the upper end of the draf plates, The electric pulses were processed by a data acquisition sistem and the cicie time distribution curves were adjusted by the Modiíied Spline fit technique. The mean of the cicie t.in e distribution creased with are increase of the bed load and the size of the ta.blets in ali comparative tests. The vibration (10 Hz, 8 mm amplitude) reduced the mean of the cicie time distribution by more than 10%, and also decreased its variance. The vibration ais() aí ected the maxirnum pressure drop (40% reduction) and the minimum spouting velocity The vibro spouted bed showedhigher- adhesion efficency (73 to 88%) than the non-vibrated spouted bed (66 to 79%) in ali equivalent situations and the complete coating of the smailer tablets dema.nded iess processing time due to higher surface arca circulation rate.
Acesso aberto (Open Access)
Influência dos frutos sobre a fixação de nitrogênio em soja (Glycine max L. Merril) cv. Santa Rosa
(Universidade Estadual de Campinas, 1987-03-25) GOMES, Maria Auxiliadora Feio; SODEK, Ladaslav; http://lattes.cnpq.br/2012001011713124
The main objective of this study was to obtain information on the relationship between reproductive development and nodule activity. For this purpose, we used nodulated soybeans induced to flower at different ages. The plants were grown in vermiculite with a nutrient solution deficient in nitrogen, and maintained in a greenhouse. Flower induction was carried out at the V2, V5 and V8 stages of development. Other treatments included the removal of flowers and the maintenance of plants in the vegetative state by growing them under long-day conditions. Measurements of nitrogen fixation were made with an intact plant system, which allows the use of the same plants throughout the growth cycle. When these measurements required the simultaneous quantification of nodule number and weight, a detached root system was used, where the shoot was sectioned immediately before the measurements. The intact and detached root systems gave similar activities for nitrogenase, showing that one system is just as reliable as the other. One advantage of the intact system is the possibility of using the same plants throughout the growth cycle. Of the experiments set up, two were carried out in the summer, where plants induces in the V2 and V5 stages produced activity curves with similar profiles and these for independent of the stage of reproductive development. This conclusion was confirmed by the data of the other treatments where fruits were removed and plants maintained vegetative under long days, since such treatments did not modify the nitrogenase activity curves. In another experiment, carried out in the autumn, plants induced at V2 and V5 produced peaks of activity at different ages corresponding to the reproductive phase, or specifically to the pod-fillinq stage. Therefore, under the conditions of this experiment at least, a clear relationship was obtained between the presence of fruits and the fall in nitrogenase activity. The different behaviour of the plants in this experiment compared with the others may be due to the less favorable conditions of light and temperature. The main conclusion reached was that the competition between the fruits and nodules for photosynthates in soybeans is not the main reason for the fall in nitrogenase activity, although it could be in less favorable conditions.
Acesso aberto (Open Access)
Manguezais do Pará: fauna de galerias perfuradas por teredo em toras de Rhizophora
(Universidade Estadual de Campinas, 1989-11-29) FERREIRA, Clara Pantoja; AMARAL, Antonia Cecília Zacagnini; http://lattes.cnpq.br/4597417616952392
In view of the large area the mangroves occupy in the State of Para’s coast their well-known importance and with the purpose to investigate their plant composition and to demonstrate the importance of the galleries bored in the logs as an habitat to several populations, periodic collects of Rhizophora lags were conducted. Measurements of temperature, pH, salinity and dissolved oxigen were accomplished. The study region was divided into 2 AREAS. The first one located at the Igarape-Curumbá mangrove (Ananindeua), where the collects were carried cut from April 1985 through January 1986 and the second in the Municípios of Benevides, Belém (Mosqueiro Island), Vigia and São Caetano de Odivelas, in which collects were carried out in July 1987 and January 1988. The mangrove stands from both AREAS are well-developped, specially those from Vigia and São Caetano de Odivelas, that are structurally the most developped. Rhizophora wangle was the dominant species in the 2 AREAS. A total of 45 species and 5022 individuals was recorded from the galleries of the 40 Rhizophora logs analysed. CRUSTACEA was the dominant taxon both for individuals and species numbers in almost all Subareas. The amphipode Grandidierella bonnieroides was the dominant species in the fauna composing 43,5% of the total number of individuals. The high in the AREA I reflects the high proport of common species among Subareas. The low values among the species of the Subareas from AREA II indicates that the composition of the fauna found inside the galleries is affected both by the physical and biological conditions to which the Rhizophora logs are submitted. The relatively high species diversity values of the 2 AREAS suggests the existence of stable conditions inside the galleries, allowing biological adaptations and interactions.
Acesso aberto (Open Access)
Metabolismo de ureídeos e asparagina durante a ontogenia do fruto de plantas de soja nodulados e não-nodulados
(Universidade Estadual de Campinas, 1982) GOMES, Maria Auxiliadora Feio; SODEK, Ladaslav; http://lattes.cnpq.br/2012001011713124
The synthesis and accumulation of reserve proteins in seeds require a large supply of reduced nitrogen. Generally, the transport of nitrogen from other parts of the plant involves a few specific compounds, the nature of which depends on the species and growth conditions. The fixation of N 2 by Rhizobium in symbiotic association with soybeans, cultivated in the absence of mineral nitrogen, leads to the incorporation of atmospheric nitrogen in ureides (allantoin and allantoic acid). These compounds form the main nitrogenous components found in the transport stream of nodulated soybeans. On the other hand, soybeans grown in the absence of Rhizobium and with NO3 nitrogen source, contain asparagine as the predominant as transport form of nitrogen. The accumulation of protein in the fruits of such plants will depend, therefore, on their ability to utilize these compounds for the synthesis of amino acids. The objectives of the present study were: first, demonstrate the presence of the enzymes presumed to be involved in the utilization of these compounds (i.e. allantoinase and asparaginase). Second, determine the effect of this drastic change in the transport of nitrogen from mainly ureides (nodulated plants) to asparagine (non-nodulated plants) on the appearance of these enzymes during fruit ontogeny specifically in the pods, cotyledons and teguments Asparaginase was assayed by a radiometric method, involving the separation of the product by chromatograpy. Allantoin was assayed by measuring the formation of the product by a differential method. Activity of both enzymes was found in all tissues of the fruit, except in the pod where no asparaginase activity was detected. Both allantoinase and asparaginase presented low activities in the tegument, producing only a small peak near the and of seed development, both for modulated and non-nodulated plants, In pods and cotyledons higher levels of allantoinase were found, especially during the phase of most active protein accumulation. It is noteworthy that the activity in the cotyledon was well above the rate of nitrogen accumulation. Asparaginase activity was also higher over this period of cotyledon development, although the level of activity was lower, being similar to the rate of nitrogen accumulation. A comparison of the activity patterns nodulated and non-nodulated plants reveals little difference except for a tendency for allantoinase to remain high (pod and cotyledons) over a longer period in nodulated plants. This occurred in spite of a shorter maturation period for fruits of nodulated plants. On the other, asparaginase (cotyledon) maintained high activity over a longer period in the non-nodulated plants. The possible significance of this effect was discussed in terms of the supply of ureides and asparagine to the fruits. As to the levels of ureides and amino acids in the various fruits tissues, the data show that the ureides, despite their association with nodule activity, were present in levels above that expected for non-nodulated plants. Of the fruit only the pods presented a high level of ureides (200 x) in nodulated plants compared to non-nodulated since low but similar levels were found ín the cotyledons and teguments of both plant types. It is notworthy that the level of free amino acids was generally the inverse of that for ureides, since higher levels were found in fruits of non-nodulated plants.

Navegar

Navegando Universidade Estadual de Campinas - UNICAMP por Institutos "Instituto de Biologia – IB/UNICAMP"