ICB - Instituto de Ciências Biológicas

URI Permanente desta comunidadehttps://repositorio.ufpa.br/handle/2011/2151

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Agora exibindo 1 - 4 de 4

Acesso aberto (Open Access)
Copaiba oil effect under different pathways in mice subjected to sepsis
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2014-08) BOTELHO, Nara Macedo; SILVEIRA, Edvaldo Lima; LOPES, Letícia Nobre; SANTOS, Felipe Augusto Folha; TEIXEIRA, Renan Kleber Costa; SILVA, Thaís Travassos da
PURPOSE: To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture. METHODS: Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals; Control group: submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed. The Kaplan-Meier curves of surviving time were realized. RESULTS: All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05). CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage.
Acesso aberto (Open Access)
Effects of hypertonic saline solution associated to remote ischemic perconditioning in kidney ischemia/reperfusion injury in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2017-03) BRITO, Marcus Vinicius Henriques; YASOJIMA, Edson Yuzur; PÉRCARIO, Sandro; RIBEIRO JUNIOR, Rubens Fernando Gonçalves; CAVALCANTE, Lainy Carollyne da Costa; MONTEIRO, Andrew Moraes; COUTEIRO, Rodrigo Paracampo; RODRIGUES, Ivone Aline da Silva; SANTOS, Hellen Aparecida Geyer dos
To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. Results: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. Conclusion:Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.
Acesso aberto (Open Access)
Intestinal intraluminal injection of glutamine increases trolox total equivalent antioxidant capacity (TEAC) in hepatic ischemia-reperfusion
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2006) SALOMÃO, Alberto Bicudo; NASCIMENTO, José Eduardo de Aguilar Siqueira do; PERCÁRIO, Sandro; GARCIA, Victor Cezar Sano; MARQUES, Nicole Ribeiro; DIAS, Claudia Cristina Gomes de Oliveira
PURPOSE: To evaluate the effects of intraluminal injection of glutamine on the serum trolox equivalent antioxidant capacity in an experimental model of ischemia-reperfusion of the liver observing the applicability of modifications on the original assay method. METHODS: Thirty Wistar rats underwent laparotomy to perform a 20 cm blind sac of small bowel and occlusion of the hepatic hilo for 30 minutes and reperfusion for 5 minutes. Into the gut sac it was injected glutamine (glutamine group, n=10) or distilled water (control group, n=10). Ten other animals (sham group) underwent laparotomy without artery occlusion. Blood samples were collected for trolox equivalent antioxidant capacity assays in different temperature conditions, reagent quantities and time for spectrophotometer readings. RESULTS: Total antioxidant capacity was significantly greater in glutamine group than in both control group (1,60[1,55-1,77] vs 1,44[1,27-1,53]) and sham group (1,60[1,55-1,77] vs 1,48[1,45-1,59]). CONCLUSION: Glutamine enhanced serum antioxidant capacity. The assay technique consistently reflected the changes in the antioxidant defenses in this experimental model.
Acesso aberto (Open Access)
Viabilidade celular da mucosa do intestino delgado de ratos, após correção de choque hipovolêmico com solução de NaCl 7,5%
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2003-08) BRITO, Marcus Vinicius Henriques; NIGRO, Amaury José Texeira; MONTERO, Edna Frasson de Souza; NASCIMENTO, José Luiz Martins do; SILVA, Paula Roberta Ferreira da; SIQUEIRA, Roberta Bianca Peres
Study the effect of the volemic correction with different solutions, in the mucous of the small bowel in rats. Were used 120 rats Wistar (Rattus norvegicus albinus), males, adults, seemingly healthy, with individual weight varying between 310 and 410g, originating from of the Instituto Evandro Chagas of Belém of Pará, submitted to an adaptation period of 15 days, receiving water and ration ad libitum, during the role experiment. For the research, ten animals were distributed, in groups and subgroups as following: Standard group (S), Shock group (Sh), Physiological Solution group (PS) and Hypertonic Solution Group (HS). The groups were divided in subgroups with 10 animals each, in agreement with the day of postoperative (DPO) foreseen for the euthanasis of the animals, (1st, 3rd or 7th DPO), being after this, picked material for cellular viability in every animals. RESULTS: The group PS took less quantity viable cells. The volemic correction with chloride of sodium solution at 7.5%, when compared the correction with chloride of sodium at 0.9% (isotonic solution), took the maintenance of larger amount of viable cells, in the small bowel in rats.

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