Teses em Neurociências e Biologia Celular (Doutorado) - PPGNBC/ICB
URI Permanente para esta coleçãohttps://repositorio.ufpa.br/handle/2011/2390
O Doutorado Acadêmico pertence ao Programa de Pós-Graduação em Neurociências e Biologia Celular (PPGNBC) do Instituto de Ciências Biológicas (ICB) da Universidade Federal do Pará (UFPA).
Navegar
Navegando Teses em Neurociências e Biologia Celular (Doutorado) - PPGNBC/ICB por Linha de Pesquisa "FISIOLOGIA DE CÉLULAS E MOLÉCULAS"
Agora exibindo 1 - 3 de 3
- Resultados por página
- Opções de Ordenação
Item Acesso aberto (Open Access) Avaliação do efeito da fração lipídica extraída de Agaricus brasiliensis antioxidante e imunomoduladora in vitro e em modelo de sepse letal em murino(Universidade Federal do Pará, 2021-01) LIMA, Kely Campos Navegantes; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390; https://orcid.org/0000-0002-3328-5650Sepsis is defined as a potentially fatal organ dysfunction caused by a dysregulated host immune response to an infection. During sepsis, dysregulation of the host response occurs with the excessive release of pro-inflammatory mediators, generation of reactive species with depletion of antioxidant defenses and cellular damage. As a result, the patient develops organ dysfunction. In this context, our group proposes the A.brasiliensis Lipid Fraction (FLAb) as a possible therapy for sepsis considering its immunomodulatory and systemic antioxidant activity in a murine sepsis model. Thus, the present study aimed to evaluate the activity of FLAb isolated in vitro and to evaluate the effect of treatment with FLAb alone or associated with the antibiotic ertapenem (F-Erta) on coagulation, antioxidant and immunomodulatory parameters in the lethal sepsis model in murine. For this, FLAb was kindly provided by Dr. Herta Dalla- Santa from UNICENTRO. In the present study, the antioxidant capacity of different concentrations of FLAb (1.25 and 5 μg/mL) was evaluated and in a RAW 264.7-Luc macrophage cell line, cytotoxicity, phagocytic capacity, nitric oxide, NF-κB activity and cytokines TNF-α and IL-6 were evaluated.The survival rates were analyzed 7 days in a model of CLP sepsis in swiss albino mice (Mus musculus), and treated with CLP+Salt (0.9%), CLP+FLAb (0.2mg/Kg), CLP+F-erta (0.2mg) /Kg; 30mg/Kg). For evaluation of on coagulation, antioxidant and immunomodulatory parameters, the mice were treated by 6 and/or 24h after CLP. In vitro, FLAb show antioxidant and anti-inflammatory activity in both concentrations. In vivo, all CLP+Salt animals died within a maximum of 48 hours while the FLAb and F-Erta treated groups survived the 7 days. During this period, clinical parameters of these animals were evaluated, the septic animals treated with saline showed piloerection, with little active level of consciousness and most of the time they were stopped in the cage, some of them had ocular secretion. In addition, animals treated with saline showed significant weight loss, reduced water and feed consumption resulting in death. The FLAb and F-Erta groups were active, with normal appearance, with normal breathing and heart rate, in addition to consuming water and food within normal limits. In the inflammatory site, peritoneal cavity, the treatment with FLAb showed an anti-inflammatory effect, decreased reactive oxygen species (ROS) and increased GSH antioxidant activity and protected from cell damage, maintaining neutrophil recruitment and nitric oxide levels (NO), reducing the bacterial load. Regarding coagulation parameters (platelet count, tp and ttpa), treatment with FLAb and F-Erta eliminated the bacterial load and protected the animals from tissue damage. In the liver, 6 hours after CLP the treatment with FLAb and F-ERTA was observed in the biochemical parameters protective effect, in addition, it presented immunomodulatory, antimicrobial and antioxidant activity preventing liver damage. In the parameters evaluated in the heart, the treatment with FLAb and F-ERTA after CLP protected the animals from cardiac damage through immunomodulatory, antimicrobial and antioxidant activity. In this sense, FLAb alone showed promise as a treatment and/or adjunct in sepsis, in addition to preventing organic dysfunction in septic animals.Item Acesso aberto (Open Access) Efeitos da suplementação com açaí clarificado (Euterpe oleracea Mart.) sobre marcadores de estresse oxidativo em pacientes com doença renal crônica em hemodiálise(Universidade Federal do Pará, 2019-06) MARTINS, Isabelle Christine Vieira da Silva; NASCIMENTO, José Luiz Martins do; http://lattes.cnpq.br/7216249286784978; https://orcid.org/0000-0003-3647-9124Item Acesso aberto (Open Access) Estudo sobre o crescimento do tambaqui (Colossoma macropomom) submetido à dieta suplementada com camu camu (Myrciaria dubia) em água corrente e aquecida(Universidade Federal do Pará, 2018-09) CANTO, Miguel Angelo de Oliveira; SALGADO, Claudio Guedes; http://lattes.cnpq.br/9601463988942971; DINIZ, Domingos Luiz Wanderley Picanço; http://lattes.cnpq.br/9601463988942971This study investigated the growth of the tambaqui (Colossoma macropomum) (Cm), an caracid teleost in the Amazon region, in the laboratory. Juvenile fish were submitted to the simulation of the natural conditions of feeding, climate and running water, corresponding to the flood and dry periods of the Amazonian hydrological cycle. In the flood, its diet is predominant of fruits and seeds, the environment is mild (28 ± 2°C) and the current is higher (0.2 to 0.3 m/s). In drought, the feed is based on animal protein, there is no current and the average temperature rises (34 ± 2°C). Therefore, the aim of this study was to investigate the effects of Myrciaria dubia (Md) on the growth of Cm in the drought or flood simulations with respect to the mild (28 ° C) or heated (34 ° C), standing or running water (0,2 or 0.3m/s), and diet with higher (45%) or lower (32%) crude protein (CP) content. For this, juveniles of Cm were acclimatized (70 or 126 days) in tanks of (310 or 500 liters) according to the experimental protocol. Protocol I: diet with fractioned daily supply (3x/day) and supplemented with Md; crude protein (45 or 32%); running water (0.2 m/s) or stopped at 28 or 34°C; analysis of muscle contents of IGF1 and total lipids. protocol II: single or fractioned offer (3x / day); standing or running water (0.3 m/s), intercalated, (12 hours) or continuous; quantification of the cavity fat mass. The results are presented on average plus or minus the standard error of the mean and compared by ANOVA plus Bonferrone post-test. Correlation test for weight, length or cavity fat versus running water; in the conditions of single or fractional supply was performed to verify interrelations between the phenomena studied. A potentiation of body mass expansion rather than length occurred by the diet supplemented with Md in standing and heated water. In contrast similar potentiation occurred for running and heated water in the 45% CP diet. In turn, lower growth performance (weight and length) was observed in the group submitted to a 32% CP diet. The running water also potentiated the accumulation of cavitary and muscular fat of total lipids, suggesting that the swimming effort demands accumulation of potential energy possibly related to the preservation of protein anabolism, since the protein content in the muscle tissue was not altered. On the other hand, the single daily supply of food was not enough to maintain the resulting growth performance to sustained swimming (running water). In the fractioned supply, the group submitted to continuous running water showed the best performance, suggesting that sustained swimming in running water may be a determinant factor for the growth of Cm if it is offered a diet with high protein content, considering the warm environment as the most favorable. Finally, the increased IGF-1 content in the muscle confirms the participation of this growth factor as the final pathway of humoral regulation of muscle hypertrophy. Hypertrophy results in increased swimming effort, and, surprisingly, in response to diet supplemented by Md.