Navegando por Linha de Pesquisa "NEUROFISIOLOGIA"

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Acesso aberto (Open Access)
Ajustes motores compensatórios após lesão isquêmica focal unilateral do trato corticoespinhal
(Universidade Federal do Pará, 2017-06-30) CARVALHO, Walther Augusto de; BAHIA, Carlomagno Pacheco; http://lattes.cnpq.br/0910507988777644; PEREIRA JÚNIOR, Antônio; http://lattes.cnpq.br/1402289786010170
The aim of this work was to develop a new model of spinal cord injury caused by focal and unilateral transient ischemia after ET-1 microinjection in the dorsal funiculus and to evaluate the sensorimotor alterations of the anterior paw of rats (Wistar). Fifty (n = 50) animals (CEPAE / UFPA protocol BIO007912), who were trained, thirty-three (n = 33) were selected to compose control (n = 15), sham (n = 6) and injury (n = 12) groups. By using a micropipette, we injected the volume of 250 nL of saline (sham) or endothelin-1 (lesion) near the medial dorsal artery of the cervical segment C4 at a depth of 1 mm from the pial surface of the spinal cord. ET-1 induced cystic cavity formation of 0.421 mm2 (± 0.035 mm2, n = 3) on the corticospinal tract and suprajacent white matter, ipsilateral to the microinjection site that can be measured in cross-sections (50 μm) stained by the Nissl technique. The motor functions of the forepaw were evaluated by specific sensorimotor tests before and after injury at 3, 7 and 14 days. The results were evaluated by the ANOVA statistical test with Tukey post-hoc analysis (α = 0.05). Our results show in pasta test that after injury there is a compensatory motor behavior in which the non-preferential forepaw assumes the functions of the preferential forepaw. The Staircase test revealed a decrease in the ability to grasp the object with the preferred paw and the Contact test showed a decrease in sensitivity of the preferred paw.
Acesso aberto (Open Access)
Ajustes posturais antecipatórios e compensatórios em idosos com e sem lombalgia
(Universidade Federal do Pará, 2021-03) GARCEZ, Daniela Rosa; CALLEGARI, Bianca; http://lattes.cnpq.br/0881363487176703; https://orcid.org/0000-0001-9151-3896; YAMADA, Elizabeth Sumi; http://lattes.cnpq.br/7240314827308306
Chronic low back pain (DLC) is associated with changes in postural control and is highly prevalent in the elderly. Research shows that aging and DLC are described as important factors that affect postural control. The postural control impairments increase the risk of falls. Researches evaluating the postural control in elderly people with DLC are still necessary for greater effectiveness in balance rehabilitation programs to prevention falls in this population. The objective of this study is to verify whether anticipatory postural adjustments (APAs) and compensatory postural adjustments (CPAs) are affected by DLC in elderly people by assessing their postural control during a self-initiated perturbation paradigm induced by rapid upper arm movement when pointing to a target. Elderly people were divided into: Group with DLC (GDLC) (n = 15) and Control Group (CG) (n = 15). The participants’ lower limb muscle onset and center of pressure (COP) displacements were assessed prior to perturbation and throughout the entire movement. T0 moment (i.e., the beginning of the movement) was defined as the anterior deltoid (DEL) onset, and all parameters were calculated with respect to it. The rectus femoris (RT), semitendinosus (ST), and soleous (SOL) showed delayed onset in the GDLC group compared with the control group: RF (control: -0.094 ± 0.017 s; GDLC: -0.026 ± 0.012 s, t = 12, p < 0.0001); ST (control: - 0.093 ± 0.013 s; GDL: -0.018 ± 0.019 s, t = 12, p < 0.0001); and SOL (control: -0.086± 0.018 s; GDL: -0.029 ± 0.015 s, t = 8.98, p < 0.0001). In addition, COP displacement was delayed in the GDLC group (control: -0.035 ± 0.021 s; GDL: -0.015 ± 0.009 s, t = 3; p = 0.003) and presented a smaller amplitude during APA COPAPA [control: 0.444 cm (0.187; 0.648); GDLC: 0.228 cm (0.096; 0.310), U = 53, p = 0.012]. The GDLC group required a longer time to reach the maximum displacement after the perturbation (control: 0.211 ± 0.047 s; GDLC 0.296 ± 0.078 s, t = 3.582, p = 0.0013). This indicates that GDLC elderly patients have impairments to recover their postural control and less efficient anticipatory adjustments during the compensatory phase. Our results suggest that people with GDL have altered feedforward hip and ankle muscle control, as shown from the SOL, ST, and RT muscle onset. This study is the first study in the field of aging that investigates the postural adjustments of an elderly population with GDLC. Clinical assessment of this population should consider postural stability as part of a rehabilitation program.
Acesso aberto (Open Access)
Análise quantitativa de neurônios imunomarcados para parvalbumina no hipocampo e núcleo magnocelular do istmo em Actitis macularius no período de invernada
(Universidade Federal do Pará, 2020-02) GUERREIRO, Luma Cristina Ferreira; DINIZ, Cristovam Wanderley Picanço; http://lattes.cnpq.br/2014918752636286; DINIZ, Daniel Guerreiro; http://lattes.cnpq.br/3269424921125406; https://orcid.org/0000-0001-7369-2165
It is already known that parvalbumin (PV) neurons have their number modified in face of social, multisensory and cognitive stimuli, both in mammals and birds. However, nothing is known about its plasticity in long-distance migratory shorebirds during wintering period. Here we investigated in four distinct temporal windows of the wintering period, the plasticity of PV neurons of two brain areas of the spotted sandpiper (Actitis macularius) which includes in its migratory journey multiple stopovers for feeding and resting. We used PV as a marker of a subpopulation of inhibitory neurons and count them in the hippocampal formation (HF) and magnocellular nucleus of tectal isthmus (IMC). Based on previous evidence that HF is involved in learning and memory and social interaction, and IMC is essential for control of head and neck and eyes movements, we tested the hypothesis that PV neurons would increase in HF and remain unchanged in IMC. For this, we used the optical fractionator to estimate cell number. Brains were processed for PV immunostaining, followed by estimates of the number of PV neurons of the areas of interest. As compared with migratory rest 1, PV neurons estimates showed significant increase in the hippocampal formation of premigration group. We suggest that parvalbuminergic neurons proliferation is part of the adaptive changes of the hippocampal circuits involved with the migratory process back to the reproductive niches in north hemisphere.
Acesso aberto (Open Access)
Atividade neuroprotetora do treinamento físico moderado contra os danos morfofuncionais cerebelares causados pelo consumo de etanol de forma intensa e episódica (Binge drinking) em ratos
(Universidade Federal do Pará, 2019-09) VIEIRA, Kátia Lamarão; LIMA, Rafael Rodrigues; http://lattes.cnpq.br/3512648574555468; https://orcid.org/0000-0003-1486-4013
Ethanol (EtOH) is a psychotropic drug, central nervous system (CNS) depressant, but widely encouraged and consumed by Brazilian society, as well as in much of the world, reflecting on a public health problem. In recent decades, teenagers have been practicing a very common practice, which is binge drinking. The harmful consumption of EtOH promotes, besides biopsychosocial alteration, the homeostatic imbalance that causes neurodegeneration and loss of function with motor disorders. In contrast, the practice of moderate physical training (MPT) has been recommended for the maintenance of physical and mental health, as well as prevention or minimization of the development of some diseases due to motor activity inducing plastic and dynamic changes in the CNS, in order to favor the neurogenesis, synaptogenesis and angiogenesis, besides contributing to the synaptic modulation. In view of the benefits of MPT, it was investigated the neuroprotective effects on motor, tissue and biochemical parameters in the cerebellum of rats exposed to binge-pattern EtOH from adolescence to adulthood. Forty male Wistar rats with 30 days old were used and divided into four groups, the control being sedentary animals and treated with distilled H2O; the trained, composed of animals exercised and treated with distilled H2O; EtOH, formed by sedentary animals and treated with doses of 3 g/kg/day EtOH, 20% (w/v); and Trained + EtOH, with exercised animals and treated with doses of 3 g/kg/day EtOH, 20% (w/v). The MPT protocol was performed on a rodent treadmill for 5 days for 4 weeks and binge-pattern EtOH doses were administered by intragastric gavage in the same weeks as the MPT. After this period, the animals were submitted to open field and beam walking behavioral tests. Then, they were euthanized for cerebellum collection, evaluating immunohistochemistry from the levels of trolox equivalent antioxidant capacity (TEAC), reduced glutathione (GSH), nitrite and lipid peroxidation (LPO); as well as Purkinje cell morphology (PC), the fraction of anti-synaptophysine (SYP) and anti-myelin basic protein (MBP) immunolabeled area. According to the result, EtOH caused severe oxidative stress and motor damage, but the execution of the MPT performed promoted neuroprotective effects in the rat cerebellum, among them, the modulation of oxidative biochemistry by the restoration of GSH levels. decreased LPO levels and increased TEAC, as well as preventing neuronal loss, synaptic vesicle damage (SYP) and myelin components (MBP). Therefore, MPT can be considered as a significant therapeutic strategy for the acquisition of redox homeostasis, avoiding oxidative biochemistry imbalance, as well as tissue and functional damage in the cerebellum of rats treated by binge pattern EtOH.
Acesso aberto (Open Access)
Avaliação neuropsicológica de deficits cognitivos em pessoas vivendo com HIV (PVHIV)
(Universidade Federal do Pará, 2024-08) GONÇALVES, Samilly Palheta; RODRIGUES, Anderson Raiol; ROCHA, Fernando Allan de Farias; http://lattes.cnpq.br/3882851981484245; https://orcid.org/0000-0002-6148-1050
The introduction of antiretroviral therapy resulted in a reduction in opportunistic infections associated with HIV, consequently reducing the incidence of most neurological diseases in HIV-positive patients. However, neurocognitive changes associated with HIV (HAND) remain at a significant prevalence, even in individuals using antiretroviral drugs. The present study aimed to evaluate possible cognitive deficits in HIV-positive patients and characterize the clinical profile of neurocognitive manifestations in the Northern region of Brazil. 30 HIVpositive patients were evaluated, treated at the Health Care Center for Acquired Infectious Diseases (CASA DIA), in Belém do Pará. It was possible to verify a decline in neurological functions, which affect the functional capacity of these PLHIV, with emphasis on the cognitive domain of memory and attention, where it was possible to identify through IADLs, IHDS and CANTAB.
Acesso aberto (Open Access)
Caracterização vocal de pacientes portadores de Lúpus Eritematoso Sistêmico (LES)
(Universidade Federal do Pará, 2017-10-18) FREITAS, Milena Silva de; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Systemic lupus erythematosus (SLE) is a chronic disease characterized by progressive tissuedamage. In recent decades, novel treatments have greatly extended the life span of SLE patients. This creates a high demand for identifying the overarching symptoms associated with SLE and developing therapies that improve their life quality under chronic care. We hypothesized that SLE patients would present dysphonic symptoms. Given that voice disorders can reduce life quality, identifying a potential SLE-related dysphonia could be relevant for the appraisal and management of this disease. We measured objective vocal parameters and perceived vocal quality with the GRBAS (Grade, Roughness, Breathiness, Asthenia, Strain) scale in SLE patients and compared them to matched healthy controls. SLE patients also filled a questionnaire reporting perceived vocal deficits. SLE patients had significantly lower vocal intensity and harmonics to noise ratio, as well as increased jitter and shimmer. All subjective parameters of the GRBAS scale were significantly abnormal in SLE patients. Additionally, the vast majority of SLE patients (29/36) reported at least one perceived vocal deficit, with the most prevalent deficits being vocal fatigue (19/36) and hoarseness (17/36). Self-reported voice deficits were highly correlated with altered GRBAS scores. Additionally, tissue damage scores in different organ systems correlated with dysphonic symptoms, suggesting that some features of SLE-related dysphonia are due to tissue damage. Our results show that a large fraction of SLE patients suffers from perceivable dysphonia and may benefit from voice therapy in order to improve quality of life.
Acesso aberto (Open Access)
Classificação de perfis de produtividade usando redes neurais artificiais a partir de registros eletroencefalográficos: uma aplicação na Mineradora Vale S.A., Complexo S11D Eliezer Batista
(Universidade Federal do Pará, 2024-01) BASTOS, Caio de Oliveira; PEREIRA JUNIOR, Antonio; http://lattes.cnpq.br/1402289786010170; https://orcid.org/0000-0002-0808-1058; GOMES, Bruno Duarte; http://lattes.cnpq.br/4932238030330851
Results and methods from neuroscience can already be applied on a routine scale. Applied neuroscience is in use, for example, to measure and study brain activity under high demand using electroencephalography (EEG). In certain work environments, human productivity is a direct function of brain activity. The intense recruitment of cognitive functions such as sustained attention and working memory influence productivity directly. The labor demands experienced by workers in mining companies are an example. This work is a part of a bigger project called “Usando Treinamento Cognitivo para o Desenvolvimento de Operadores de Alto Desempenho” from the Vale S. A. mining company and, therefore, all of the workers that participated in this study worked for that company. We used EEG to measure productivity during a task without the constraints typically found in experiments conducted in the laboratory. To this aim, we created a machine learning algorithm to analyze the resting EEG recorded before and after a 4D simulation, where mining workers (37 ± 7 years old) specialized to operate giant high-capacity shovels. The simulated task consisted of the operator using the shovel in a digging and loading routine. The task was not planned for our research. It was part of the worker’s routine training. That is, we took advantage of the workers’ training to carry out the study. Recordings were preprocessed using a band-pass filter (0.5-100 Hz) followed by filtering using ICA (Independent Component Analysis). After each operator finish the simulation, their productivity was measured by VALE S.A technical staff. The main parameter for good productivity was the amount of ore excavated. The operators were divided into groups according to ranges of productivity. The productivity was used as a label for the learning of the algorithm that consisted of an artificial neural network of the type inception. The number of neurons and layers was optimized using Bayesian optimization. The features extracted by the inception were the input to 13 classifiers. The classifier chosen for the final algorithm was the best, that is the one providing the best accuracy in the productivity classification. The training set contained 80% of the data. A hold-out validation was used to test the accuracy of the final algorithm using 20% of the data. The resulting accuracy when the operators were divided into four groups of productivity reached 91.35%. When there were only two groups of productivity the accuracy peaked at 95.05%. Our results showed that even under no laboratory constraints – during the regular training of the operators and using resting EEG – our algorithm succeed and it is ready to be used in future field operations. We have a prototype that is patentable.
Acesso aberto (Open Access)
Correlação entre medidas quantitativas de espessura retiniana, concentração de metabólitos encefálicos e funções neuropsicológicas de pacientes com transtornos neurocognitivos
(Universidade Federal do Pará, 2017-10-17) WAN-MEYL, Fabio da Silva; TEIXEIRA, Cláudio Eduardo Corrêa; http://lattes.cnpq.br/7448998858430931; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Currently, with the increase in life expectancy of human populations, neurodegenerative diseases, commonly occurring with the advancing age, have become a source of serious concern. Thus, research into new ways of early diagnosis of neurocognitive disorders associated with diseases such as Alzheimer's and Parkinson's, as well as the improvement of the understanding of currently available clinical neurological diagnostic methods, has been promoted throughout the world. In this sense, this work proposes to investigate the existence of possible correlations between the results of exams used in clinical neurological investigation of patients diagnosed with neurocognitive disorder associated with these important neurodegenerative diseases (Alzheimer's and Parkinson's diseases). The results show that the measurement of the thickness of the nerve fiber layer of the perimacular retina, performed by optical coherence tomography, is a parameter that may not differ significantly between groups of patients and healthy subjects. On the other hand, the measurement of the amplitude of the spectroscopic signals generated by encephalic metabolites, performed by magnetic resonance proton spectroscopy, reveals encephalic changes that vary from region to region. In addition, the neuropsychological measure of cognitive functions, performed by the automated CANTAB battery, reveals that several aspects of these functions are impaired in these patients. Finally, Principal Component Analysis shows that, considering the set of variables obtained by tomographic and neuropsychological measurements, it is possible to observe a correlation between several of these variables. Thus, it is concluded that correlating the results obtained by different approaches may add potential in the interpretation of this casuistry, which would not be possible if we consider such data in an isolated way.
Acesso aberto (Open Access)
Desenvolvimento de aplicativo para realização do finger tapping test e a influência do sexo e da dominância manual no desempenho do finger tapping test baseado em smartphone
(Universidade Federal do Pará, 2023-11) BRITO, Felipe André da Costa; SOUZA, Givago da Silva; http://lattes.cnpq.br/5705421011644718; https://orcid.org/0000-0002-4525-3971
The Finger Tapping Test (FTT) is a classical neuropsychological test that assesses motor functioning, and recently it has been employed using smartphones. For classical protocols, it has been observed that sex and handedness influence the performance during the test. By assessing the influence of sex and handedness on the test, it is possible to adjust the performance measurements to ensure the validity of test results and avoid sex- and handedness-related bias. The present study aimed to evaluate the influence of sex and handedness on smartphone-based FTT performance. We developed an Android application for the FTT and recruited 40 males and 40 females to carry out three spatial designs on it (protocols I, II, and III). Participants’ performance was measured using the global, temporal, and spatial parameters of the FTT. We observed that for the performance in protocol I, handedness had a significant influence on global and temporal variables, while the interaction between handedness and sex had a greater influence on spatial variables. For protocols II and III, we observed that handedness had a significant influence on global, temporal, and spatial variables compared to the other factors. We concluded that the smartphone-based test is partly influenced by handedness and sex, and these factors should be considered during the evaluation of the smartphone-based FTT.
Acesso aberto (Open Access)
Dinâmica de sucção disfuncional em lactentes com anquiloglossia
(Universidade Federal do Pará, 2023-11) CUNHA, Bruna Mendes Lourenço; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Introduction: Breastfeeding is widely recommended as an exclusive diet until 6 months of age and continued until two years or more. Its benefits are numerous and proven, including improving the immune system, a nutrient-rich diet and craniofacial development from the sucking movement. However, not all babies are able to breastfeed due to some difficulties, with ankyloglossia. Known as tongue tie, it can restrict tongue movements, making breastfeeding difficult and, in some cases, being responsible for early weaning. Objective: Compare infant suction in babies with and without ankyloglossia using a microprocessor-controlled pressure sensor coupled to a pacifier. Method: Fifty-five infants from 0 to 2 months of age underwent clinical examination for ankyloglossia, after which they were offered a silicone pacifier connected to the pressure acquisition device and suction activity was recorded. Thus, we extracted the frequency of sucks within a burst, the average suck duration, the burst duration, the number of sucks per burst, the maximum amplitude of sucks per burst and the inter-burst interval. Results: The key difference in newborns with ankyloglossia in relation to control was that they perform longer bursts of suction activity. Conclusion: The longer burst durations are likely a compensatory strategy and may underlie the pain reported by mothers during breastfeeding. We therefore propose a method for objectively quantifying some parameters of infant suction capacity and demonstrate its use in assisting the evaluation of ankyloglossia.
Acesso aberto (Open Access)
Distribuição espacial e temporal das redes perineuronais durante o desenvolvimento pós-natal do córtex pré-frontal
(Universidade Federal do Pará, 2022-04) COIMBRA, Gabriele dos Santos; BAHIA, Carlomagno Pacheco; http://lattes.cnpq.br/0910507988777644; https://orcid.org/0000-0003-3794-4710
The mammalian prefrontal cortex (PFC) is the cerebral cortex area involved in processing several functions as cognition and complex motor control for social interactions. In this PFC area, there is no duration of the time window definition about its critical period of plasticity. One of the potential biological markers for this may be the Perineuronal Nets (PNNs). The present work aimed to examine the developmental time course of PNN formation focusing on the medial prefrontal cortex (mPFC) of rats using histochemistry with Vicia villosa agglutinin. We use 21 male rats Rattus novergicus, wistar lineage, which were randomly divided into seven experimental groups, composed of 3 animals in each group, as follows: group at 7, 14, 20, 26, 58, 75, and 135 postnatal days, respectively. We found that in PFC, PNNs appear at P26 with a small number of Vv+ cells, increasing in total numbers until adulthood. The results of the present study demosntrate the temporal development of PNN formation in the Wistar rats mPFC, and we suggest a time window for the end of the critical period of plasticity in this cortical area (26- 75 postnatal days), there is a progressive decrease in PNNs with immature profile and a concomitant increase in mature PNNs during postnatal development of the mPFC, making this PNNs profile more prevalent at more advanced ages, around 3 months of age, when the animals are already considered young adults.
Acesso aberto (Open Access)
Efeito da competência leitora nas estratégias de organização perceptual da cena visual em escolares
(Universidade Federal do Pará, 2023-02) RESQUE, Deusa Priscila da Silva; MATOS, Felipe de Oliveira; http://lattes.cnpq.br/4207066833785568; https://orcid.org/0000-0002-4926-4694; PEREIRA JUNIOR, Antonio; http://lattes.cnpq.br/1402289786010170; https://orcid.org/0000-0002-0808-1058
The current Brazilian educational scenario, evaluated by different national and international indicators, has shown alarming results in mathematics, science and especially in reading. The country is in the last positions in all evaluations. For the change of this scenario, urgent educational measures that respect the student's biological and social development are necessary. The applied neurosciences to education emerge as a theoretical model of aid for pedagogical practices that aim to improve quality in education. An important contribution of neurosciences in education is focused on reading, through a theoretical/practical contribution on brain functioning those points more effective paths for literacy with a view to reading competence. In this sense, it was possible to understand that the human species has not yet developed a specific neural system for reading, using other brain structures for this function, ie literacy depends on a neural recycling process of brain structures associated with Sensory, motor and language functions, as is the case with brain regions associated with faces recognition, to accommodate the decoding of the written word. The aim of this study is to investigate reading in school -age children. To this end, the present thesis divided into two studies: Study 1 that investigates the inhibition of the generalization of letters in mirror in school -age children and the study 2 that investigates the relationship between reading competence and perceptual organization strategies of the visual scene in schools . Both studies were conducted in three public schools in the Belém do Pará Metropolitan Region, Brazil. Altogether two hundred and thirty -one subjects aged 6 to 10 years (113 boys, 8.2 ± 1.4 years and 118 girls, 8.2 ± 1.4 years), enrolled 1 to 5th years, were recruited, 1,2 ± 1.4 years old) elementary School. Among these eighty -eight subjects were students from school 1, fifty -two from school 2 and ninety from the school 3. In all, six tests were applied, each school participated in a different group of tests. Our results show that the performance of boys in the 6-10 year old is different from that of girls in the same age group for the reading competence test, with boys with higher performance, although it is not possible to establish differences between genders to the perceptual closure test. What's more, while boys performed significantly better in the rotation of letters in the older group, girls' performance was similar in both groups. This pattern is reverse for the mirror's task, with the older girls surpassing their younger counterparts and the boys performing similarly in both groups. Since the age period of our sample is not associated with large variations in reproductive steroid levels, we propose that the similarity of performance between younger and older girls in the mental rotation of letters may be associated with society's traditional attitudes and expectations and expectations about the relationship between spatial visual skills and gender. As for the mirror's task, while only girls showed a significant difference between the two age groups, the boys showed improvement, as expected by the mirror generalization inhibition to letters during reading. These findings are relevant to the development of more effective future strategies for optimizing the literacy process of school -age children, combining education and neuroscience. For this, more studies must be performed and the number of subjects investigated should be expanded.
Acesso aberto (Open Access)
Efeito do tratamento combinado com laserterapia e gel de açaí na fase proliferativa de reparo em modelo de lesão por ruptura tendínea total em ratos
(Universidade Federal do Pará, 2023-08-13) MENDES, Aurea Gabriela Rodrigues; MORAES, Suellen Alessandra Soares de; http://lattes.cnpq.br/6278397231382779; BATISTA, Evander de Jesus Oliveira; http://lattes.cnpq.br/2206444845201080
Acai extract has been investigated as a pharmacological strategy due to its anti- inflammatory, antioxidant and antinociceptive effects. Furthermore, studies report that low-intensity laser (LBT) may favor tissue repair due to its proliferative effects in increasing the number of fibroblasts and collagen synthesis. Therefore, the combined use of açaí with LBT may be a therapeutic alternative in the management of tendon repair. The aim of this study was to evaluate the effects of combined treatment with açaí gel and LBT on the morphofunctional and nociceptive aspects of Achilles tendon injury in the proliferative phase of repair. For this, 40 Wistar rats were divided into control group (CTRL), injury (INJURY), 10% açaí gel (INJURY+GA), 2J infrared laser, (INJURY+LAS) and combined treatment (INJURY+LAS+GA). The Von Frey test, Joint Angle and Achilles Functional Index were performed at 0-, 3-, 7- , and 14-days post-injury (DPL). After 14 days, the tendons were collected and the tissue organization pattern was analyzed by H&E or Alcian Blue to assess variations in glycosaminoglycans in the extracellular matrix, staining with DAPI to quantify the number of cells and collagen autofluorescence to assess the orientation pattern of collagen fibers. We demonstrated improvement in the macroscopic characteristics of the tendon in the treatment groups in relation to INJURY, which may be correlated with better organization and tissue orientation, as well as collagen fibers in these groups. The increase in the number of cells and fibroblasts was more evident in the laser group, emphasizing the proliferative role of the laser. Functional recovery was observed in 7 DPL in the isolated laser group, while the combined treatment improved nociception in the acute phase (7th DPL). In the proliferative phase (14th DPL), the IFA showed improvement in functional aspects in the combined treatment group. On the other hand, nociceptive sensitivity was reestablished in all treatment groups. In summary, our findings indicate that the combined use of açaí gel with LBT may be a therapeutic alternative in the management of tendon injury repair.
Acesso aberto (Open Access)
Estimulação transcraniana por corrente contínua em esportes de combate: efeito sobre o desempenho físico e cognitivo
(Universidade Federal do Pará, 2021-11) LOBÃO, Thais Alves; GOMES, Bruno Duarte; http://lattes.cnpq.br/4932238030330851; GOUVEIA JUNIOR, Amauri; http://lattes.cnpq.br/1417327467050274
Transcranial direct current electrical stimulation (tDCS) is a non-invasive neuromodulation technique used to promote improvement in clinical symptoms of neurodegenerative diseases. There is evidence that tDCS could modulate the psychomotor abilities of athletes, which are important for the performance of these athletes. Among the combat sports, judo and jiu-jitsu are two types that represent fights with specific physical and cognitive demands. Thus, the aim of this study was to investigate the effects of a single stimulation in the M1 region (primary motor), on cognitive (anxiety, reaction time) and physical (strength, muscle power, flexibility) parameters in federated male athletes of these modalities. For that, the fighters were submitted to two experimental sessions of tDCS (sham and stimulated, 2 mA for 20 minutes), in a cross-over scheme (ABA), composed by psychomotor evaluations using as measures the jump against movement (JAM), Wells bank, manual and scapular dynamometry, IDATE (state-trait anxiety index) and reaction time, through the TReaction software. There were no statistically significant differences between pre- and post-stimulation conditions (sham or anodic) and in the percentage of difference between the two pre- and post-test conditions. Our results indicate that there are no effects of tDCS in this region and in the values used on the measured parameters.
Acesso aberto (Open Access)
Estressores agudos distintos produzem diferentes magnitudes do comportamento tipo de ansiedade e liberação diferencial de glutamato no cérebro de zebrafish
(Universidade Federal do Pará, 2024-08) MARTINS, Milena Letícia; SILVA, Anderson Manoel Herculano Oliveira da; http://lattes.cnpq.br/8407177208423247; https://orcid.org/0000-0003-4022-8096
Anxiety disorder is one of the most well characterized behavioral disorder in individuals submitted to acute or chronic stress. However, few studies have demonstrated how different types of stressors can modulate the neurochemical alterations involved in generation of anxiety. In the present study, we hypothesize that subjects exposed to different aversive stimuli (mechanical, chemical and spatial restriction) present varied intensity of anxiety-like behavior response associated with distinct pattern of GABA and Glutamate release in the brain. Sixty adult Danio rerio animals were randomly divided into four experimental groups: Control (CTRL), Acute Restraint Stress (ARS), Conspecific Alarm Substance (CAS) and Chasing with Net (CN). After applying the stress protocols, the animals were individually transferred to the Novel Tank Diving Test for behavioral analysis. Subsequently, their brains were collected and subjected to GABA and Glutamate release assay for quantification by HPLC. Our behavioral results showed that all aversive stimuli were capable of inducing anxiety-like behavior. However, the impact of anxiogenic behavior was more prominent in the CN and CAS groups when compared to ARS. This phenomenon was evident in all analyzed behavioral parameters (time on top, freezing, mean speed, maximum speed and erratic swimming). Our data also have shown that all aversive stimuli induced significant decrease in GABA release when compared to the control group. On the other side, only animals exposed to CN and CAS presented increase in extracellular glutamate levels. Different acute stressors induce different intensity of Anxiety-like behavior in zebrafish as well as they provoke specific alterations on the GABAerigic and Glutamatergic release in the brain. These results demonstrate the complexity of anxiety disorders, highlighting that both behavioral and neurochemical responses are highly context-dependent, supporting our initial hypothesis.
Acesso aberto (Open Access)
Estudo do desenvolvimento auditivo da habilidade de fusão binaural
(Universidade Federal do Pará, 2022-04) ARAÚJO, Francisca Canindé Rosário da Silva; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Introduction: Binaural Interaction (BI) allows the introduction of auditory information (in the brain as a function of differences in perception of intensity or time of acoustic stimuli). Allows you to assess the action and integrated co- operative of the brainstem in lower understanding. As the maturation of the central nervous system occurs in the craniocaudal direction, the response to this ability may change during the course of development. Objectives: To normalize and compare the development with increasing age of response in the binaural diffusion test (BPT) with digital low-pass (LP) and high-pass (HP) filters in normative listeners. Methods: Prospective, cross-sectional and observational study. A total of 120 years were evaluated, in different age groups (6 to 8 years old, 10 to 12 years old, 14 to 16 years old and 20 to 30 years old) with TFB, filter at Fc 500/1700 Hz digital Finite Impulse Response type order 4096, with null phase and 5000 between 18 and 30 years with unfiltered speech material. Results: a progressive improvement in performance with increasing age (ANOVA (one-way): p<0.0001). There was a significant difference between the filtered words, age and the unfiltered p < (Dunnet: any filtered words,01). The difference between the age groups was significant (Tukey: p<0.01), less for the results obtained in the age groups of 6-8 and 10-12 years and of 14-16 and 18- 30 years. Discussion: IB is a skill that evolves with age development and NC fabrication. Final considerations: The interpretation of the TFB should take into account the performance by age group of the patients. This is important for future applications of these tests in people with Auditory Processing Disorder.
Acesso aberto (Open Access)
Estudo eletrorretinográfico da adaptação à luz de vias de processamento específicas de cone e de oponência em cor e luminância
(Universidade Federal do Pará, 2017-12-15) COSTA, Alódia Brasil; SILVA, Anderson Manoel Herculano Oliveira da; http://lattes.cnpq.br/8407177208423247
Visual system has an essential function called light adaptation or photopic adaptation, which consists in the regulation of light sensitivity allowing visual adaptation to a wide range of illumination levels. This phenomenon is not fully elucidated. Therefore, the present study aims to evaluate the magnitude and temporal pattern of the variation of retinal electrical responses for L cone and M cone selective stimuli and for luminance pathway and red-green color pathway specific stimuli, making possible the evaluation of the red-green parvocellular (P pathway) and magnocellular (M pathway) visual pathways activity, during the photopic adaptation. For such purpose, 6 healthy subjects underwent 30 minutes of dark adaptation and then were exposed a light steady background for 16 min. The stimuli were given every 2 min and the electrical retinal response was recorded by full field electroretinogram (ffERG) over 16 min. The triple silent substitution method was used to isolate the responses of pathways originating from the L or M cone. We also used specific stimuli of luminance (Lum) and red-green color (Crom). For each stimulation type was used intermediate (12 Hz, which reflects the activity of the red-green parvocellular pathway - P pathway) and high (36 Hz, which reflects the activity of the magnocellular pathway - M pathway) temporal frequency, resulting in 8 stimulation conditions. Amplitude and phase of the first (F), second (2F) and third (3F) harmonic components were extracted by Fast Fourier Transform. It was observed that amplitude and phase increased over light adaptation time with simple sine wave form in the most of components and stimulation conditions. The relative increases in F amplitude from the M-cone driven responses during the light adaptation were higher than those L-cone driven ERGs at both temporal frequencies, 12 Hz (M = 1.21, L = 0.33) and 36 Hz ( M = 1.94, L = 0.55), as well as they were higher at 36 Hz than at 12 Hz for the two cones. In general, there was slight F phase increase during the light adaptation time (<30 degrees), slightly higher at 36 Hz. Regarding the light adaptation kinetics, F amplitude and phase that seem reflect the activity of the P pathway presented a faster adaptation (L-cone 12 Hz, M-cone 12 Hz, Crom 12 Hz, Lum 12, with a mean of 1.4 min) and those that probably reflect activity of the M-pathway had slower adaptation (Lum 36 Hz, L-cone 36 Hz, M-cone 36 Hz, with a mean of 4.9 min). Thefore, M and P pathways presented different magnitude and kinetics of light adaptation, being the M pathway the pathway of greatest increase and slower adaptation.
Acesso aberto (Open Access)
Expressão gênica durante o desenvolvimento ocular e regulação de assimetria de opsinas na espécie Anableps anableps, peixe de quatro olhos
(Universidade Federal do Pará, 2022-11) SOUSA, Daniele Salgado de; SCHNEIDER, Patrícia Neiva Coelho; http://lattes.cnpq.br/9584217233879031
The ocular development is a complex process orchestrated by several events that include: cell specification, morphogenesis and differentiation. All these developmental and functional processes are extremely conserved among living vertebrate species, however, unique adaptations are sometimes observed, such as in fish of the genus Anableps. Unlike most fishes, which use their eyes to explore a submerged world, in Anableps anableps (Anablepidae: Cyprinodontiformes), the eye is adapted for the simultaneous perception of a world above and below the water line. These exceptional adaptations include: duplicated corneas and pupils, as well as a specialized retina region associated with simultaneous aerial and aquatic vision, and which have asymmetrical gene expression. Recently, by transcriptomic analysis of the developing eyes of A. anableps, 20 non-visual opsin genes were identified to be asymmetrically expressed between pre- and post-duplication stages of corneas and pupils. Thus, here, we analyzed by in situ hybridization the gene expression of a bistable opsin (parapinopsin) and a neuropsin (Opn5) in A. anableps larvae. Our data showed that the pattern of gene expression of these opsins is symmetrical between the dorsal and ventral retina, respectively, with expression in the ONL, INL and GCL layers. We also investigated the expression of three non-visual melanopsin genes (opn4x1, opn4x2, opn4m3), one eleost multiple tissue opsin (tmt1b), and two visual opsins (lws and rh2-1) in the dorsal and ventral retinas of juvenile A. anableps, after changing the photic conditions in which the juvenile fish were. Then, we showed that in the transition from an environment of high turbidity to one of clear water, opsins have their expression patterns changed. Additionally, by immunofluorescence, we revealed the expression of Lamin A/C, proteins expressed as part of eye development in A. anableps as well as in other developing organisms, as well as in adult individuals. Therefore, we believe that the information described here elucidates many aspects of the molecular mechanisms behind the development and adaptive plasticity of A. anableps’ eyes.
Acesso aberto (Open Access)
O extrato aquoso de Coriandrum sativum L. promove neuroproteção contra alterações motoras e danos oxidativos na progênie de ratas expostas ao metilmercúrio
(Universidade Federal do Pará, 2020-01) RODRIGUES, Keuri Eleutério; PRADO, Alejandro Ferraz do; http://lattes.cnpq.br/7016475842644161; NASCIMENTO, José Luiz Martins do; http://lattes.cnpq.br/7216249286784978; https://orcid.org/0000-0003-3647-9124
This study aimed to investigate the efects of Coriandrum sativum aqueous extract (EACS) on the rat progeny of mothers exposed to methylmercury (MeHg). The presence of bioactive compounds and EACS's antioxidant capacity been evaluated, and the spring were assessed for their total mercury levels, motor behavioral parameters and oxidative stress in the cerebellum. The analysis of the bioactive compounds revealed significant amounts of polyphenols, flavonoids, and anthocyanins, as well as a variety of minerals. A DPPH test showed the EACS had important antioxidant activity. The MeHg + EACS group performed significantly better spontaneous locomotor activity, palmar grip strength, balance, and motor coordination in behavioral tests compared the MeHg group, as well as in the parameters of oxidative stress, with similar results to those of the control group. The MeHg + EACS group alsohad significantly reduced mercury levels in comparison to the MeHg group. Based on the behavioral tests, which detected large locomotor, balance, and coordination improvements, as well as a reduction in oxidative stress, we conclude that EACS had positive functional results in the spring of rats exposed to MeHg.
Acesso aberto (Open Access)
Função de recuperação do nervo auditivo após doze meses de uso do implante coclear
(Universidade Federal do Pará, 2022-03) YAMAGUCHI, Cíntia Tizue; SILVA FILHO, Manoel da; http://lattes.cnpq.br/2032152778116209
Introduction: Cochlear implant is the standard treatment for severe and profound bilateral sensorineural hearing loss. To optimize the adaptation of this device, especially in patients who do not have the ability to refer to the parameters necessary for programming, such as children and people with associated disabilities, objective tests based on the action potential of auditory nerve fibers have been studied as possible predictors. of these parameters to be used in cochlear implant programming. The auditory nerve recovery function is a test that measures the time the auditory nerve needs to recover from a stimulus (leave the absolute refractory period) to receive new stimulation and possibly be more responsive to the auditory sensation offered by the cochlear implant. Objective: Through a specific software for cochlear implants, with the objective of measuring the time constant (τ) in the intraoperative period and in the postoperative period after 12 months of cochlear implant use in children. Method: We recruited children with cochlear implant and intraoperative neural response, evaluated the recovery function using commercially available cochlear implant software. Data were collected intraoperatively and repeated twice 12 months after surgery. Results: We found that the recovery time of the auditory nerve increases after 12 months of cochlear implant use. Our results also show that the profile of temporal responses is significantly higher in the postoperative measurement than in the intraoperative one. The test-retest reproducibility of the composite evoked action potential recordings proved to be reliable and stable. Conclusion: There was a change in 12 months of cochlear implant use, in relation to the same measure at the intraoperative moment in the subjects of this study. The intraoperative τ measurement was faster, however, in the postoperative period the mean showed higher τ values. However, the current level was different intraoperatively and 12 months after use, needing to be further explored. There was no statistical difference regarding the test-retest in the postoperative period, showing reliability and reproducibility of the measurement. Possibly it would be the beginning of the study of a responsiveness profile in relation to the recovery time of the auditory nerve.