Navegando por Assunto "AINEs"

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Acesso aberto (Open Access)
Avaliação da presença de antiflamatórios não esteroides no sistema de abastecimento de água Bolonha Belém/PA
(Universidade Federal do Pará, 2022-04-12) RIBEIRO, Caroline Mascarenhas; TEIXEIRA, Luiza Carla Girard Mendes; http://lattes.cnpq.br/9773700229115395; https://orcid.org/0000-0002-0204-6825
The present study aimed to investigate the occurrence of non-steroidal antiinflammatory drugs - NSAIDs in the Bolonha Complex, Belém-Pa, from water samples collected in the Bolonha Reservoir (raw water) and after the filtration and disinfection of the ETA Bolonha, in addition to verifying possible relationships between the NSAIDs in the study and the water quality parameters. The determination of NSAIDs was based on the solid phase extraction method, on GC-MS (Gas Chromatography coupled to mass spectrometry) for the identification of the compounds Ibuprofen (IBU) and Paracetamol (PAR), and LCMS/MS (Chromatography Liquid coupled to sequential mass spectrometry) to obtain Naproxen (NAP) and Diclofenac (DFC). The results obtained indicated the presence of the four drugs PAR, IBU, NAP and DFC in the Bolonha Complex. At point AB, the first three drugs, respectively, had an annual mean of 3.7 ng/L, 9.1 ng/L and 351.8 ng/L. At point AF, the same drugs obtained, in the same order, annual mean concentrations of 14 ng/L, 231.2 ng/L and 1932.2 ng/L. At point AT, only IBU and DFC were detected, with annual averages of 0.9 and 35.9 ng/L, respectively. It was found that the main step responsible for the elimination of drugs was chlorination, especially in the rainy season, equivalent to an annual removal efficiency of 100% in the cases of PAR and NAP, and 94%, referring to the IBU. In the correlations, no strong relationship was identified between the drugs detected in the raw (AB), filtered (AF) and treated (AT) water points. At Point AB, the approximation of the NAP with the pH color, apparent color, total iron, turbidity and E-coli variables stands out. At point AF, the same drug showed a correlation with the apparent color in the rainy season, however in the dry season it did not show strong correlations with any of the variables analyzed. At point AT, the DFC stood out with the approximation of the pH, turbidity and alkalinity variables. In general, the assessment of the presence of NSAIDs proved to be important for a better understanding of the issue of microcontaminants in the Amazon and, in addition, confirmed the hypothesis of the presence of NSAIDs in different parts of the ETA Bolonha, which may be related to the discharge of untreated sewage from the neighboring population
Acesso aberto (Open Access)
Inibição de ciclooxigenase-2 (COX-2) em camundongos infantis saudáveis: consequências sobre o comportamento e o perfil oxidativo
(Universidade Federal do Pará, 2022-08-26) LIMA, Klinsmann Thiago; BASTOS, Gilmara de Nazareth Tavares; http://lattes.cnpq.br/2487879058181806
In the central nervous system, cyclooxygenase 2 (COX-2) is a constitutive enzyme, expressed by neurons from different brain regions, which acts in the maintenance of neural homeostasis, modulating synaptic plasticity and the generation of new neurons. Non-steroidal anti-inflammatory drugs (NSAIDs) are drugs of choice that act to inhibit COX enzymes, with nimesulide (NMS) being a drug of this class. Several studies have demonstrated the role of these enzymes in neurological and neuropsychiatric disorders such as Parkinson's Disease, Alzheimer's Disease, epilepsy, depression and schizophrenia. Thus, the aim of the present work was to investigate the effects of COX- 2 inhibition in healthy infant mice on behavioral and biochemical criteria, using NMS as a pharmacological blockade tool. For this, male Swiss infant mice, aged between 21 and 34 days, were used. The animals were randomly divided into four groups: (1) Vehicle, (2) NMS 2.5mg/kg, (3) NMS 5mg/kg and (4) NMS 10mg/kg. Two injections of NMS/Vehicle were administered intraperitoneally daily. Throughout the experiment, the body mass of the animals was recorded daily and they were subjected to behavioral tests: open field test (OFT), elevated plus maze (EPM), light/dark box test (LDBT) and novel object recognition test (NORT). In addition, brain samples were collected for biochemical analyses. The results demonstrated the induction of oxidative stress with increased levels of lipid peroxidation in the cortex and hippocampus, as well as the expression of an anxiogenic behavior, observed in the EPM, possibly potentiated by fear. In the NORT, the animals of the NMS 5mg/kg group showed a deficit in the memory of recognizing new objects, and consequently, in the short-term memory. Thus, our results demonstrated that the in vivo inhibition of COX-2 in infant animals induces an anxious-like behavior possibly potentiated by fear, but does not affect the exploration and locomotion of these animals. Furthermore, COX-2 inhibition induced cortical and hippocampal oxidative stress. Therefore, the inhibition of COX-2 in infantile and non-inflamed animals may compromise cognitive functions such as memory and learning, as well as alter the cerebral oxidative profile.