Navegando por Assunto "Acmella oleracea"

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Acesso aberto (Open Access)
Avaliação do teor de espilatol no ciclo de cultura de duas cultivares de Aemella oleracea (L.) R. K. Jansen em extratos obtidos por entração supercritica
(Universidade Federal do Pará, 2018-12-10) SILVA, Ana Paula de Souza e; FERREIRA, Gracialda Costa; http://lattes.cnpq.br/4250668524181387; CARVALHO JÚNIOR, Raul Nunes de; http://lattes.cnpq.br/5544305606838748; https://orcid.org/0000-0002-4433-6580
Acmella oleracea (L.) R. K. Jansen is a species native to South America, popularly known as jambu, and has been consumed for many years as an ornamental, medicinal, and food plant. To obtain espilantol, the main bioactive compound of this species, various extraction techniques have been used in order to obtain the isolated substance, since it is not commercially available. Among the techniques, supercritical carbon dioxide extraction stands out as it showed high selectivity for espilantol, with yields above 50%, resulting in a purity above 90% of the isolated compound. The objective of this work was to evaluate the influence of rainfall and harvest time on the yield of spilanthol with supercritical CO2 in two cultivars of Acmella oleracea (L.) R. K. Jansen flowers, as well as to evaluate their pharmacological effect. For this purpose, the following procedures were performed: raw material characterization; optimization of the process variables (flow and time); obtaining of extracts by supercritical extraction; determining of the process cost on an analytical scale; determination of the total phenolic compounds concentration in the extracts by Folin-Ciocalteu method; spilanthol content quantification in the extracts by gas chromatography coupled to mass spectrometry, and evaluation of its antinociceptive in vivo activity. The main results were: the mineral analysis indicated high levels of iron, calcium, zinc, and magnesium; the process optimization resulted in a CO2 use decrease from 955,8g to 477,9g, and reduced extraction costs from R$ 20.47 to R$ 11.45. The higher espilantol content was 29.22%, while the highest phenolic content was 43.04%. The flowers of both Acmella oleracea cultivars were considered good sources of iron, calcium, zinc, and magnesium. The highest values of extract yield, spilanthol content and total phenolic compounds were obtained in the rainy season and in the shortest harvest times. Between the two cultivars evaluated, no significant statistical differences were found.
Acesso aberto (Open Access)
Utilização de técnicas hifenadas para o desenvolvimento de programações para analise de Acmella oleracea (L.) RK Jansen
(Universidade Federal do Pará, 2016-08-31) SILVA, Ianna Dias Ribeiro da; REBELO, Monaliza Maia; http://lattes.cnpq.br/2064401199679916; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070
The use of hyphenated techniques that allow the separation and isolation of substances of a herbal extracts is necessary both for the knowledge of the chemical composition of a compound, as well as for the determination of a substance or a group that serve as marker of particular species. The species Acmella oleracea (l.) R. K. Jansen, grows in Legal Amazon, a geographic region in northern Brazil, where is known as Jambu, Agrião Bravo ou Agrião do Pará. It is used in folk medicine in the form of tea, syrups and tinctures and indicated for use as anesthetic and antiseptic. The aim of this study was to use the HPLC-UV-ELSD and HPLC-DAD-MS for the development of methods that can identify metabolites of interest on Acmella oleracea. The plant material was acquired in Tailândia, State of Pará, in the Santa Inês farm, city of Tomé-Açu, PA150 highway margins, positioned the 260 km from Belém, at 02° 56 ' 22 '' south latitude and 48° 57 ' 03 '' west longitude, and consisted of 10 packs of Acmella oleracea (jambu), composed of leaves, flower, root and stem, after cleaning the material was grinded and then macerated for 7 days with 92.8° GL ethanol at a ratio of 1:10. Hydroethanolic extracts, of each part of the plant, were filtered and concentrated at a low pressure and then lyophilized for further analysis with hyphenated techniques. The Spilanthol was registered in the analysis by HPLC-DAD-ESI-MS, Scan mode and Product ion, through the detection of ion m/z 222 in all analyzed organs. A substance which showed m/z 376.80 for analysis with etoh/water and m/z 388.20 for acn/water, which has promising profile was found through the analysis by HPLC-DAD-MS and needs specific experiments for confirmation of flavonoids. During the development of methods, it was observed that the best chromatographic profiles of EEAOFOC and EEAOR were obtained when the mobile phase was composed of ethanol and water.