Navegando por Assunto "Agentes anti-inflamatórios"

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Acesso aberto (Open Access)
Efeitos anti-inflamatórios e neuroprotetores do extrato de cipó-pucá (Cissus verticillata) após isquemia focal induzida por microinjeções de endotelina-1 (ET-1) no córtex motor de ratos adultos
(Universidade Federal do Pará, 2018-12-19) COSTA, Jonabeto Vasconcelos; LEAL, Walace Gomes; http://lattes.cnpq.br/2085871005197072
The inflammatory response may exacerbate the damaging process after acute neural disorders such as in stroke. Alternatives to obtain a decrease in the inflammatory response in the encephalic cell accident have been widely studied with the use of herbal compounds, in this hypothesis the pucá cucó (Cissus verticillata), an Amazonian medicinal plant popularly used as anti-inflammatory and antihyperglycemic used by folk medicine in the treatment of acute inflammatory diseases. However, there are no investigations into the possible anti-inflammatory and neuroprotective effects of plant extract in experimental models of acute neural disorders. In this study, we investigated the anti-inflammatory and neuroprotective effects of plant extract by supercritical extraction in adult rats submitted to acute injury induced by endothelin-1 (ET-1) in the motor cortex. Two experimental groups were delineated: the first with animals of the control group with a survival time of twenty-four hours and seven days (Group N = 3 for each survival time), submitted to focal ischemia with ET-1, but injected 5% tween intraperitoneal (ip), and the second group of animals treated with doses of 100 mg / kg (ip) of plant extract after surgery with the same survival times (Group N = 5 for each survival time). Then perfused twenty four hours and seven days after induction of ischemic injury. General histopathological analysis was performed in sections stained by cresyl violet and hematoxylin. Neutrophils and macrophages were identified by immunohistochemistry with specific antibodies (anti-MBS1 and IBA-1, respectively), astrocytes labeled with anti-GFAP antibody. Activated microglia/ macrophages and neuronal bodies were counted in the mentioned experimental groups and the astrocyte activity after the lesion was evaluated. Treatment with Cissus verticillata extract induced anti-inflammatory and neuroprotective effects in treated animals, as well as decreased tissue cavitation, astrocyte activation at the center of the lesion and decreased infiltration of polymorphonuclear and/or microglia/ macrophage inflammatory cells.
Acesso aberto (Open Access)
Neuroproteção, diminuição do infiltrado de neutrófilos e microgliose após tratamento com óleo-resina de copaifera reticulata ducke em um modelo experimental de lesão aguda da medula espinhal
(Universidade Federal do Pará, 2017-01-25) SANTOS, Thayssa Ferreira dos; LEAL, Walace Gomes; http://lattes.cnpq.br/2085871005197072
Most central nervous system (CNS) diseases are incurable and extremely debilitating, acute spinal cord injury (LAME) causes permanent loss of neurological function below the level of the injury, causing severe physical consequences to patients. Trauma, falls and gunshot or joint weapon damage together make up almost 100% of the main mechanisms that cause LAME, as well as contribute to the high costs of the public health system. The pathophysiology of LAME involves complex processes such as vascular changes, excitotoxicity, lipid peroxidation and neuroinflammation, generated mainly by microglial cells. Despite the knowledge of pathophysiology, there is still no effective treatment for LAME. Thus, there is a mobilization of the scientific community to find a substance capable of promoting neuroprotection and, consequently, to reduce the sequelae of LAME below the level of the lesion. In this context, the Copaíba Resin Oil may represent a good therapeutic strategy. In this study, we investigated the antiinflammatory and neuroprotective effects of copaiba resin oil after hemisection of the spinal cord of rats. The animals were divided into experimental and control groups. Immunohistochemical techniques using anti-MBS-1 (neutrophil marker), anti-Iba-1 (microglial marker) antibodies, as well as staining with Cresila Violet were used. Qualitative and quantitative analyzes were performed. Copaiba resin oil proved effective in decreasing the recruitment of inflammatory cells to the area of spinal cord injury and promoted better preservation of the tissue area compared to the control group. As in the lowest neutrophil recruitment in treated rats compared to the control group (Treated group: 8.33 ± 0.66 (N = 3); Control group: 12.27 ± 0.28 (N = 3)) . Copaíba resin oil also promoted a reduction in the number of microglia in the area of spinal cord injury at different times (Treated group on day 1: 8.59 ± 1.72 (N = 3), Control Group on day 1: 35, (N = 3), Control Group on the 7th day: 65.77 ± 6.19 (N = 3)). These results suggest the antiinflammatory and neuroprotective effect of copaiba resin oil after LAME, revealing a promising strategy for the patient after LAME.