Navegando por Assunto "Amor-crescido"

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Acesso aberto (Open Access)
Atividade antibacteriana de plantas medicinais frente á bactérias multirresistentes e a sua interação com drogas antimicrobianas
(Universidade Federal do Pará, 2012-08-28) SARAIVA, Rosa Márcia Corrêa; VIEIRA, José Maria dos Santos; http://lattes.cnpq.br/6807452375674442
Infection control of the multidrug-resistant microorganisms sometimes is ineffective even with the development of new antibiotics. Many herbal extracts have antimicrobial effects and may represent an alternative therapy for infectious diseases, mainly when associated with antibiotics of clinical use. The aim of this study was to evaluate the antibacterial activity of medicinal plants in multidrug-resistant microorganisms and their interaction with antimicrobial agents. We evaluate the antibacterial activity of plant extracts and fractions of Eleutherine plicata (“marupazinho”) Geissospermum vellosii (“pau-pereira”) and Portulaca pilosa (“amor-crescido”) against isolates of Oxacillin-Resistant Staphylococcus aureus (ORSA) and multi-resistant bacteria Pseudomonas aeruginosa, from human clinical isolates. Also we evaluate interaction of these plant extracts with antimicrobial agents of clinical use. The antibacterial activity was determined by disk diffusion on Mueller Hinton agar and the Minimum Inhibitory Concentration (MIC) by micro dilution plate technique using Muller Hinton broth as culture medium and 0.01% resazurin as a developer of bacterial growth. The extracts and fractions were tested at concentrations of 500, 250, 125, 62.5, 31.2 and 16.2 μg/mL dissolved in 10% DMSO. Plants E. plicata and G. vellosii demonstrated activity against ORSA isolates with MICs of 125 μg/mL, whereas P. pilosa had an effect on the isolates of P. aeruginosa with MIC of 250 μg/mL. There were 25% of synergism and only 5% of antagonism of all 120 plant and antimicrobial agents interaction tested. ORSA isolates had synergistic interaction with ciprofloxacin, clindamycin and vancomycin agents and with both plant derivatives of E. plicata and G. vellosii. The derivatives of P. pilosa potentiated the action of the aztreonam, cefepime and piperacillin + tazobactam agents compared to the isolates of P. aeruginosa multidrug-resistant. The results shows therapeutic potential of E. plicata, G. vellosii and P. pilosa in the control of bacterial infections involving multidrug-resistant phenotype (MDR) and its interaction with antibacterial agents may represent a new alternative in the therapy of these infections.
Acesso aberto (Open Access)
Avaliação das atividades antinociceptiva e anti-inflamatória do extrato hidroetanólico de partes aéreas de Portulaca pilosa L. (Portulacaceae)
(Universidade Federal do Pará, 2012-08-30) FERREIRA, Fabrício Alexopulos; DOLABELA, Maria Fâni; http://lattes.cnpq.br/0458080121943649; SOUSA, Pergentino José da Cunha; http://lattes.cnpq.br/9909053957915090
This study investigated the acute oral toxicity, the antinociceptive effect in chemical and thermal nociception models as such as the anti-inflammatory activity in carrageenan and croton oil models of the hydroethanolic extract from aerial parts of Portulaca pilosa (HEEPp). Also identified some possible mechanism involved in antinociception of the extract as such as the effects of HEEPp on central nervous system of rats. In the oral acute toxicity test, the treatment with HEEPp ( 2000 mg/kg) caused no deaths. In the acetic acid-induced writhing test, the HEEPp (100, 200, 400 and 600 mg/kg) administered by oral route (p.o.) significantly reduced the number of contortions acetic acid-induced in 18.18, 33.25, 47.27, 65.81 e 73.94%, respectively. In the hot plate test, the treatment with HEEPp (200, 400 e 600 mg/kg, p.o.) did not alter the latency to the thermal stimuli of 50 ± 0,5 ºC. In the formalin test, the treatment with HEEPp (200, 400 e 600 mg/kg, p.o.) significantly reduced the licking-time in neurogenic phase (first phase) in 38.79, 60.61 and 75.18 %, and inflammatory phase (second phase) in 49.23, 53.03 e 87.53 %, respectively. The previous naloxone administration, significantly reversed the effect of HEEPp (600 mg/kg, p.o.) in both phases of the formalin test. The pre-treatment with L-NAME and methylene blue significantly reversed the effect of HEEPp (600 mg/kg, p.o.) in both phases of the formalin test. The pre-treatment with glibenclamide also significantly reversed the effect of HEEPp (600 mg/kg, p.o.) in both phases of the formalin test. HEEPp (600 mg/kg, p.o.) did not affect the locomotor activity of rats in the open field test. In the carrageenan-induced paw edema and croton-induced ear edema, the HEEPp (400 and 600 mg/kg, p.o.) did not inhibit significantly the edema formation in both the tests. The results of this study showed that HEEPp, when administered by oral route, presented low toxicity and its antinociceptive actuation observed in neurogenic phase involves peripherals interaction with opioids receptors and activation of the in the NO/GCs/GMPc/ KATP pathway. Already the antinociceptive activity observed in the inflammatory phase does not seem to depend of the inhibition on via phospholipase A2/cyclooxygenases, but interaction with peripheral opioid receptors and the NO/sGC /cGMP/ KATP pathway.