Navegando por Assunto "Annona glabra"

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Acesso aberto (Open Access)
Efeitos citoprotetor e citotóxico de Annona glabra (Annonaceae)
(Universidade Federal do Pará, 2016-10-03) SARMENTO, Rosana Moura; SILVA, Jaqueline Rodrigues da; http://lattes.cnpq.br/8336745480297714; DOLABELA, Maria Fâni; http://lattes.cnpq.br/0458080121943649
The present study evaluated the cytotoxic and cytoprotective potential of ethanolic extract obtained from the shells of Annona glabra, its fractions and isolated substances. The powder obtained from A.glabra husks was subjected to maceration with ethanol for 7 days, and the solution was concentrated in a rotavaporator to residue. The ethanolic extract from A.glabra was partitioned between aqueous hexane: methanol (9: 1). The methanolic fraction was fractionated in chromatographic column using as Sephadex stationary phase and mobile phase the methanol. The cytotoxicity of the ethanolic extract and fractions was evaluated by the MTT cell viability assay ([3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide]). The extract, fractions and subfractions were submitted to thin layer chromatography (CCD) analysis, and pooled according to similar characteristics. The 50% cytotoxic concentration (IC 50) was determined by linear regression. Fractions of the extract with IC50 ≤ 30 μg / mL and isolated substance with IC50 ≤ 4 μg / mL are considered cytotoxic. Fractions with moderate to low cytotoxicity were submitted to the induction of apoptosis and DNA fragmentation by flow cytometry. Also, these samples were submitted to evaluation of oxidative stress by the TEAC and DPPH method. The extract of A. glabra (8.39% yield) was partitioned to give the methanolic fraction (yield 88.14%) and hexane fraction (yield 8.08%). Ethanolic extract, methanolic fraction and rutin showed low cytotoxicity (IC50 = 137.7, 139.4,> 200 μg / mL, respectively). Hexanic fraction and subfractions 17 and 19 showed moderate non-significant cytotoxicity (IC50 = 45.07, 53.45, 80.65 μg / mL, respectively). All the evaluated samples did not induce apoptosis cells, however, ethanolic extract, hexane fraction and rutin promoted changes in the cell morphology. However, hexanic fraction, subfractions 6 and 7 showed the ability to fragment DNA from cells. The fractionation of the ethanolic extract favored the cytotoxic potential, with the hexane fraction being the most promising, and the antioxidant capacity was also favored, with group 5 being the most promising. These results suggest that A. glabra samples have low cytotoxic potential, and the mechanism involved is not related to the induction of apoptosis, and the ethanolic extract contains substances with antioxidant capacity.
Acesso aberto (Open Access)
Estudos farmacognósticos, fitoquímicos e biológicos de Annona glabra L. (Annonaceae)
(Universidade Federal do Pará, 2016-05-12) BRÍGIDO, Heliton Patrick Cordovil; MARINHO, Moacir do Rosario Marinho; http://lattes.cnpq.br/2511998363000599; DOLABELA, Maria Fâni; http://lattes.cnpq.br/0458080121943649
In this study, the Annona glabra underwent pharmacognostic, phytochemicals and biological studies (leishmanicide and antimicrobial activity). In pharmacognostic studies, we used the methods described in Brazilian Pharmacopoeia V ed. (2010). The ethanolic extract (EE) was obtained by maceration of the powder batch of shells with ethanol. The extract was fractionated by liquid-liquid partition with hexane and 10% aqueous methanol resulting in hexane (HF), and methanol (MF) fractions. The MF was submitted to Sephadex column. This procedure resulted in 46 fractions that were analyzed in thin layer chromatography and revealed with sulfuric acid, Dragendorff, and ultraviolet (360 nm) being assembled into 5 groups according to their chromatographic profiles. Group 3 was purified by column chromatography on a preparative scale yielding the G3-1 sample. EE, MF, HF, Group 2 and G3-1 were analyzed by HPLC-DAD. The G3-1 sample was analysed by mass spectrometry and nuclear magnetic resonance (NMR). To evaluate the antimicrobial activity, methods of agar diffusion (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa) and microdilution (MIC) were used. The EE and its fractions were subjected to leishmanicide activity test (Leishmania amazonensis). The powder was classified as coarse and low-density, with ash and moisture contents within the parameters established by the Brazilian Pharmacopoeia. In HPLC-DAD, the main peaks of EE and its fractions were presented in UV absorption spectrum of 240 nm to 280 nm, and 300 nm to 400 nm suggestive respectively Band II (Ring A), and band I (ring B ) of flavonoid. The G3-1 chemical structure was identified as flavonoid rutin. In the agar diffusion test, we observed the formation of halos in EE and MF only in Staphylococcus aureus plates. In the microdilution assay, the EE and FM showed MIC> 1000 mg / mL, considered inactive. In antileishman test, the EE showed IC50> 200 / ml. The MF and HF also showed IC50> 200 / ml; however, they inhibited the growth of promastigotes respectively in 20% and 33.7%. The subtractions and G3-1 Group 2 showed IC50> 200 / ml, but the concentration of 200 / ml inhibited the parasite growth by approximately 45%. The EE, fractions, and subfractions were inactive against L. amazonensis amastigotes. However, the HF concentrations of 250 and 125 g / ml inhibited infection in 39.1% and 18.7%. In short, EE and its fractions were shown to be inactive in the antimicrobial and leishmanicide trials, but fractionation contributed to increase activity suggesting that active substances must be at low levels in extract and its fractions.