Navegando por Assunto "Antimicrobial activity"

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Acesso aberto (Open Access)
Avaliação da composição química, atividade antioxidante e antimicrobiana do óleo de cipó-de-alho (Mansoa standleyi) (Steyerm.) A. H. Gentry (Bignoniaceae) obtido via extração supercítica
(Universidade Federal do Pará, 2019-05-31) URBINA, Glides Rafael Olivo; CARVALHO JÚNIOR, Raul Nunes de; http://lattes.cnpq.br/5544305606838748; https://orcid.org/0000-0002-4433-6580
Mansoa standleyi is popularly known as "cipó-de-alho" because of its characteristic garlic odor (Allium sativum). The plant has an inhibitory effect on bacteria and fungi growth. The objective of this work was to evaluate the chemical composition, antioxidant and antimicrobial activity of the oil obtained from M. standleyi leaves via supercritical fluid extraction (SFE) and conventional extraction (Hydrodistillation). The SFE tests were performed using supercritical CO2, static extraction time of 30 min and dynamic time of 180 min, CO2 flow of 2.5 L/min and raw material mass of 10 g. The temperature and pressure variables were selected in order to obtain a difference in the CO2 density (𝜌𝐶𝑂2) in the 35 and 45 ºC isotherms combined with the pressure values of 100, 200, 300, and 400 bar. The results obtained allow us to verify that the global yield values of SFE on dry basis show significant differences between them (p≤0.05), with values varying from 0.87 to 2.02%. The highest average found refers to 400 bar/35 ºC condition (𝜌𝐶𝑂2 = 972.26 kg/m3). However, in Hydrodistillation extraction the oil yield was 0.14%. The results of phytochemical screening by high performance thin layer chromatography (HPTLC) evidenced the presence of terpenes, fatty acids, phenolic compounds, and flavonoids. It was demonstrated the presence of substances with antioxidant activity for all oils obtained with SFE, being observed a better identification in the oils obtained at high pressures. In the quantitative analysis of chemical composition, it was observed that the values ranged from 31.87 to 72.06 mg EAG/g oil in the content of total phenolic compounds. In relation to the antioxidant activity, the values ranged from 457.64 to 2475.55 EC50 expressed in g of oil/g of DPPH, the best results being obtained in the condition of 400 bar/35 ºC. In the fatty acid profiles of M. standleyi oil obtained by SFE, the results indicated that there was a difference in the fatty acids qualitative composition detected, in function of the operational extraction condition, where the presence of linoleic (≅95%), palmitic (≅12%), and oleic (≅5%) acid was observed. The chemical constituents identified in the obtained oil by Hydrodistillation were identified as sulfur compounds, phenols, and alcohols. The antimicrobial activity in vitro analysis showed that the oil obtained by SFE presents an antibacterial action against Stapphylococcus aureus and Escherichia coli. Finally, M. standleyi oil represents an alternative for future use in the treatment of diseases caused by microorganisms.
Acesso aberto (Open Access)
Efeito de uma espécie do gênero Varronia sobre a viabilidade celular, atividade antimicrobiana, toxicidade dérmica aguda e o processo de cicatrização (in vitro e in vivo)
(Universidade Federal do Pará, 2019-09-09) RIBERA, Paula Cardoso; FONTES JÚNIOR, Enéas de Andrade; http://lattes.cnpq.br/7056265073849866; https://orcid.org/ 0000-0002-6186-9581
Tissue damage, particularly to the skin, results in damage to cell structures, layers, and lineages to the fullest extent. Under these conditions, wound healing is the physiological process responsible for tissue repair. Inflammation is an important stage in tissue repair and; therefore, a strong target for clinical studies. The species Varronia multispicata is popularly used for the treatment of bruises, with recently discovered anti-inflammatory and analgesic effects. The present work aimed to investigate the effect of aqueous extract of Varronia multispicata leaves (VAR01) on cell viability, antimicrobial analysis, dermal toxicity, and in vitro and in vivo healing. In the in vitro assays, there were evaluated the cell viability in BALB/c 3T3 murine fibroblasts, the antimicrobial action by the microdilution method for determination of minimum inhibitory concentration and petri dish culture technique for the minimum bactericidal concentration. Healing assays were performed in cultured fibroblast monolayers. For in vivo assays in the dermal toxicity test, female Wistar rats were used and divided into the following groups: saline, 100mg/ml, 200mg/ml, and 1000mg/ml of VAR01; as for healing evaluation, Mus musculus mice were used and divided into 4 groups: sham, negative control, treated (VAR01 10%), and positive control (Dersani®). V. multispicata kept the cells viable for 24h, with reduction of fibroblasts in the 48h period at a concentration of 500 µg/ml. It showed no antimicrobial activity, presented in vitro and in vivo injury contraction capacity, did not promote death or behavioral changes, did not cause changes in water and feed intake, weight gain, relative weight, and organ histological analysis, showed a reduction in alkaline phosphatase concentrations in the group treated with 100 mg/ml extract when compared as control group. It was also revealed a reduction in alanine aminotransferase levels in the 100 mg/ml extract-treated group when compared to the control group. However, a significant increase in TGP concentrations was found in the 200 mg/ml group when compared to the control group. While assessing the degree of irritation, VAR01 did not show an irritant profile when administered acutely topically. Therefore, the extract is safe and of low toxicity, promising in the process of tissue regeneration with possible modulation in the inflammatory pathway, being a stimulating result for the following steps of biological activity evaluation and elucidation of the healing process.