Navegando por Assunto "Arritmias cardíacas"

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Acesso aberto (Open Access)
Apocinina reverte fibrose e disfunção elétrica cardíaca induzida pelo aumento sistêmico de MMP-2 em camundongos adultos
(Universidade Federal do Pará, 2024-09) PONTES, Maria Helena Barbosa Pontes; RODRIGUES, Keuri Eleutério; http://lattes.cnpq.br/0030683756521893; PRADO, Alejandro Ferraz do; http://lattes.cnpq.br/7016475842644161; https://orcid.org/0000-0001-7495-9837
Heart failure (HF) is characterized by the heart's inability to maintain adequate tissue blood flow, associated with deficits in contraction and relaxation, due to either an acute or chronic injurious event. Matrix metalloproteinase 2 (MMP-2) is linked to the pathogenesis and pathophysiology of HF, promoting proteolysis of contractile proteins and oxidative stress. Rescue therapies that directly or indirectly modulate MMP-2 activity could help improve cardiac remodeling and dysfunction. Apocynin inhibits NADPH oxidase, thereby attenuating oxidative stress. This study hypothesizes that apocynin can reverse cardiac remodeling and electrical dysfunction induced by MMP-2 by preventing oxidative imbalance. The aim of this study is to evaluate the effect of apocynin on oxidative imbalance and cardiac remodeling induced by systemic MMP-2 increase in adult mice. Adult male C57BL/6 mice were subjected to two experimental protocols. First, the animals underwent a time course protocol and were divided into two groups: the vehicle group received 0.9% saline via intraperitoneal (ip) injection, and the MMP-2 group received MMP-2 (150 ng/g body weight) via ip injection, for up to 4 weeks. Subsequently, a treatment protocol with apocynin was performed, starting 4 weeks after the cessation of MMP-2 administration. During this period, the animals were divided into four experimental groups: 1) vehicle (received water via gavage); 2) apocynin (50 mg/kg via gavage); 3) MMP-2 (received water via gavage); and 4) MMP-2 + apocynin (50 mg/kg via gavage). At the end of the protocols, all animals underwent electrocardiography, and then their hearts were collected for morphological and biochemical evaluation. During the time course, the MMP-2 group showed increased gelatinolytic activity, oxidative imbalance, fibrosis, decreased heart rate, along with increased RR, PQ, QT, and QTc intervals from the first week of administration, effects that persisted over the four weeks, even without MMP-2 administration. Treatment with apocynin reversed the increase in MMP-2 activity and expression in the heart, as well as oxidative imbalance, lipid peroxidation, hypertrophy, cardiac fibrosis, and electrical dysfunction. We conclude that systemic MMP-2 increase can promote cardiac remodeling through increased MMP-2 activity and expression in cardiac tissue, leading to redox imbalance and electrical dysfunction, and that apocynin treatment was able to reverse the effects induced by MMP-2, suggesting that these effects are dependent on oxidative imbalance and NADPH oxidase.
Acesso aberto (Open Access)
Classificação de arritmias cardíacas através de uma estrutura competitiva de redes neurais convolucionais autoassociativas
(Universidade Federal do Pará, 2023-05-11) CORRÊA FILHO, Sérgio Teixeira; CASTRO, Adriana Rosa Garcez; http://lattes.cnpq.br/5273686389382860
This work proposes a system for classifying cardiac arrhythmias based on a competitive structure of Autoassociative Convolutional Neural Networks. Three neural networks were trained to reconstruct Electrocardiogram (ECG) signals for cases of patients with supraventricular, ventricular and normal beats. After training, the networks were allocated in a competitive parallel structure for classification of arrhythmias. The MIT-BIH arrhythmia public database of ECG signals was used for training and testing the networks, and for each ECG signal, from each patient, the QRS complexes of the heartbeats were extracted, which were the characteristics used as input. for the system, and these signals, which were in the form of temporal signals (1D), were transformed into digital images (2D) in order to use the capacity of convolutional neural networks for pattern recognition and feature extraction in images. For the development and performance analysis of the proposed structure, two paradigms that have been used in works already presented in the literature were used: interpatient paradigm and intrapatient paradigm, and the system obtained an accuracy of 96.97%, sensitivity of 96.30% and precision of 93.59% for the intrapatient case and accuracy of 94.05%, sensitivity of 70.43% and precision of 65.74% for the interpatient case. A comparative analysis with results from arrhythmia classification systems already presented in the literature shows that the proposed system presented similar results or, in some cases, better results than those already obtained, thus showing the applicability of the proposed structure to the problem