Navegando por Assunto "Ayapana triplinervis"

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Acesso aberto (Open Access)
Avaliação da atividade cicatrizante do extrato hidroalcoólico de Ayapana triplinervis (Vahl) R.M. King & H. Robinson (ASTERACEAE)
(Universidade Federal do Pará, 2020-07-16) NASCIMENTO, Suellen Carolina Martins do; LIMA, Anderson Bentes de; http://lattes.cnpq.br/3455183793812931; https://orcid.org/ 0000-0002-0534-2654; ANDRADE, Marcieni Ataíde de; http://lattes.cnpq.br/8514584872100128; https://orcid.org/ 0000-0001-5875-695X
Wound healing is a complex process that involves the organization of cells, chemical signals and remodeling in order to repair injured tissue. North American statistical data show a prevalence of skin lesions in approximately 22.8% of the world's population. In Brazil, wounds are a serious public health problem. In this sense, the use of medicinal plants as therapeutic agents has aroused interest among researchers due to their most different effects, including healing. Thus, ethnopharmacological studies are found in the literature, which associate the use of A. triplinervis with wound healing, but there is no scientific evidence to prove this activity. Thus, this study aims to evaluate the healing potential of the hydroalcoholic extract of A. triplinervis incorporated in an ointment of 5 and 10%. For that, phytochemical analyzes were carried out: prospecting of the plant drug and the hydroalcoholic extract, by means of colorimetric, precipitation tests, thin layer chromatography and high-performance liquid chromatography of the hydroalcoholic extract. In addition to the macroscopic, morphometric analysis, and histological examination of the hydroalcoholic extract ointment in 5% and 10%, using Dersani® and saline solution, respectively, as positive and negative controls. Through these tests, it was observed that the plant drug presented several compounds: saponins, anthraquinones, steroids and triterpenes, polyphenols and coumarins. For the hydroalcoholic extract the result was similar, but the tests did not indicate the presence of anthraquinones. Analysis by thin layer chromatography of the hydroalcoholic extract revealed positive results for coumarins, steroids and triterpenes. In the chromatographic profile, the extract, obtained by high performance liquid chromatography, showed a peak, being suggestive of coumarin. Macroscopic analysis of the lesions showed that the groups of the 5% and 10% extract ointments had more re-epithelialized wounds. In morphometry there was no statistical difference between the four test groups in the percentage of wound contraction. However, the histological examination showed that the ointment with 10% hydroalcoholic extract showed better quality in the development of tissue repair, as it increased fibroblasts, collagen, keratinization, more than the other groups. Thus, this study showed that A. triplinervis extract ointment did not accelerate the speed of wound closure, however, it did show a beneficial influence on the quality in which the lesions evolved, yet further research is necessary to better understand It is made.
Acesso aberto (Open Access)
Avaliação do potencial microbicida das folhas da espécie Ayapana triplinervis Vahl.
(Universidade Federal do Pará, 2013-12-06) LOPES, Tamyris Regina Matos; GONÇALVES, Ana Cristina Baetas; http://lattes.cnpq.br/6886126078022769; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390
Acesso aberto (Open Access)
Isolamento e avaliação antitumoral de aiapina em tumor ascítico de ehrlich
(Universidade Federal do Pará, 2019-08-30) OKABE, Daniele Hidemi; LIMA, Anderson Bentes; http://lattes.cnpq.br/3455183793812931; https://orcid.org/ 0000-0002-0534-2654; ANDRADE, Marcieni Ataíde de; http://lattes.cnpq.br/8514584872100128; https://orcid.org/ 0000-0001-5875-695X
Ayapana triplinervis is an Amazonian plant species, popularly known as japan, which has coumarins as important chemical components, having its presence in relation to their pharmacological effects. Raw extracts of species have shown important antitumor effect. Therefore, this study aimed to isolate an A. triplinervis coumarin to evaluate its antitumor effect on ehrlich ascites tumor. For this, extracts of different polarities were captured from aerial parts of the plant and performed in phytochemical prospecting of plant medicines and extracts used. The best profile extract for coumarins was selected for the relationship by Column Chromatography. The coumarin fraction was subjected to a relationship and an isolated substance was identified by chromatographic methods and spectral. The acute toxicity and antitumor effects of coumarin were evaluated in mice, attracting the evaluation of body weight and cell viability in animals, intraperitoneally with 10, 20 and 40 mg / kg of ayapina. Phytochemical assays showed the presence of saponins, steroids and triterpenes, anthraquinones, polyphenols and coumarins. The ethyl acetate extract was selected for the relationship, without qualifying 28 fractions. As fractions obtaining or better selection profile for coumarins, they were from Fr 12 to 20. As fractions were pooled and refracted for coumarin isolation. Through the analysis it was possible to confirm the isolation of coumarin and identify it as 6,7- (methylenedioxy) coumarin, known as ayapina. Coumarin was considered to have low potential in Mexico. Aiapine showed antitumor effect with intraperitoneal treatment in mice with ascending tumor at doses of 10, 20 and 40mg / kg, by reducing the weight of animals and cancer cells without ascites fluid. Thus, the present study demonstrates for the first time the potential antitumor effect of ayapina.