Navegando por Assunto "Bancos de sangue"

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Acesso aberto (Open Access)
Nota técnica n. 02/2022/UFPA/PPGAC. Critérios para o melhor aproveitamento de bolsas de concentrado de hemácias de baixo volume
(Universidade Federal do Pará, 2022) ESTÁCIO, Adriana Guimarães; BRITO JUNIOR, Lacy Cardoso de; http://lattes.cnpq.br/9705670940390281
Acesso aberto (Open Access)
Nota técnica n. 03/2022/UFPA/PPGAC. O impacto da automação na fenotipagem eritrocitária estendida para a rotina de bancos de sangue
(Universidade Federal do Pará, 2022) CARNEIRO, Luciana Corrêa; BRITO JUNIOR, Lacy Cardoso de; AMARAL, Carlos Eduardo de Melo
Acesso aberto (Open Access)
Perfil de expressão diferencial de microRNAs durante o armazenamento prolongado de concentrados de plaquetas em banco de sangue
(Universidade Federal do Pará, 2019-02-28) MAUÉS, Jersey Heitor da Silva; BURBANO, Rommel Mario Rodriguéz; http://lattes.cnpq.br/4362051219348099
Platelet concentrate (PC) is a key blood component, which even in good storage conditions, susceptible to cellular damage over time. Hence, blood banks discard unused PC bags after 5 days of storage. Biomarkers of PC quality are therefore highly sought after in blood bank governance. We used the data (Gene Expression Omnibus: GSE61856) generated with next-generation sequencing to examine the expression profiles of microRNAs (miRNAs) from PCs that were stored for 6 days in a blood bank, that is, 1 day longer than is normally stored PC. We identified the 14 most differentially expressed miRNAs by comparing a control PC on the first day of storage with the PCs on each of the subsequent 5 days of storage from day 1 to 6. In all, we identified nine miRNAs with the downregulated profile (miR-145-5p, miR-150-5p, miR-183-5p, miR-26a-5p, miR-331-3p, miR-338-5p, miR-451a, miR-501-3p, and miR-99b-5p) and five upregulated miRNAs (miR-1304-3p, miR-411-5p, miR-432-5p, miR-668-3p, and miR-939-5p). These miRNAs were validated by real-time quantitative PCR in 100 PC units. As each PC unit is composed of platelets of five individuals, the validation was thus performed in 500 individuals (250 men and 250 women, comprised 18–40 years old adults). The data were analyzed with hierarchical clustering and principal component analysis, which revealed the variation of mean relative expression and the instability of miRNAs half-life on the fourth day of PC storage, which coincides with time of onset of platelet storage lesions. These new observations can usefully inform future decision-making and governance in blood banks concerning PC quality.