Navegando por Assunto "Bauhinia guianensis"

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Acesso aberto (Open Access)
Chemical constituents of Aspergillus sp EJC08 isolated as endophyte from Bauhinia guianensis and their antimicrobial activity
(2013) PINHEIRO, Eduardo Antonio Abreu; CARVALHO, Josiwander Miranda; SANTOS, Diellem Cristina Paiva dos; FEITOSA, André de Oliveira; MARINHO, Patrícia Santana Barbosa; GUILHON, Giselle Maria Skelding Pinheiro; SANTOS, Lourivaldo da Silva; SOUZA, Afonso Duarte Leão de; MARINHO, Andrey Moacir do Rosário
The present work reports the isolation of five compounds from Aspergillus sp EJC08 isolated as endophytic from Bauhinia guianensis, a tipical plant of the Amazon. The compounds ergosterol (1), ergosterol peroxide (2), mevalolactone (3), monomethylsulochrin (4) and trypacidin A (5) were isolated by chromatographic procedures and identified by spectral methods of 1D and 2D NMR and MS. Compounds 3, 4 and 5 were tested against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and showed good activity.
Acesso aberto (Open Access)
Estudo químico e atividade antibacteriana do fungo endofítico Scedosporium apiospermum de Bauhinia guianensis e de fungos da Serra de Carajás
(Universidade Federal do Pará, 2016-06-22) CORDEIRO, Jorgeffson da Silva; MARINHO, Patricia Santana Barbosa; http://lattes.cnpq.br/4826647905254039; MARINHO, Andrey Moacir do Rosario; http://lattes.cnpq.br/2511998363000599
Endophytic fungi are microorganisms that lives in association with the host species, and they're a promising source of important natural products. With a equal importance, the soil fungi also are capable of producing so many substances of economic value. Thus, the following work was objective to obtain bioactive composts produced by endophytic fungi M. acutistipula var. ferrea and soil Carajás Saw, besides the endophytic S. apiospermium from liana of Bauhinia guianensis Auble. The species M. acutistipula var. ferrea, were isolated 56 endophytic and 64 soil fungi. They were randomly, selected, 12 fungi to be reactivated in Petri dishes containing PDA medium. After reactivating the micro-extracts of 12 fungi were obtained as the adapted methodology Smedsgaar. The micro-extracts were evaluated by HPLC-DAD to determine the chromatographic profile and, also, submitted antimicrobial testing. The FSF 12 (trichoderma sp.), selected after the screening of chemical and biological profile was grown on a large scale in a solid medium (rice) and led to the isolation of five substances: fatty acid polyunsaturated (E1), 5'-inosil (E2), tyrosol (E3), harzialactone A (E4) and 2-anhydro mevalonic (E5). In relation to the fungus EJCP13 (S. apiospermium) where compounds isolated, triacyl (J1), ergosterol peroxide (J2), ergosterol (JA19), cerivisterol (JA29), methylester (JA24), dulcitol (D), and brefeldin A (JA). The compound Brefeldin A showed significant antimicrobial activity and, also, proved to be the major secondary metabolite in extracts from S. apiospermium. Thus, it decides to quantify a substance in the extract, which corroborate results, which indicate the presence of brefeldin A only in AcOEt and the hexane extracts. The isolation of the substances was performed by chromatographic techniques, such as CCVU and, monitoring, by ADCC. The chemical structures were elucidated with the aid of spectroscopic (1H and 13C NMR, HMQC, HMBC), and spectrometric (ESI-MS).
Acesso aberto (Open Access)
Perfil cromatográfico e potencial químico e biológico de fungos de solo e fungos endofíticos isolados de Bauhinia guianensis e Mimosa acutistipula var. Ferrea
(Universidade Federal do Pará, 2016-03-04) PINHEIRO, Eduardo Antonio Abreu; MARINHO, Andrey Moacir do Rosario; http://lattes.cnpq.br/2511998363000599; MARINHO, Patrícia Santana Barbosa; http://lattes.cnpq.br/4826647905254039
The study of natural sources and that exhibit little explored specific ecological interactions their habitat has been emphasized as a strategy for the discovery of new substances with biological activities. In this context, this study aims to carry out the chemical and bioactive study of endophytic fungi and the Amazon soil fungi, to contribute in the search for bioactive compounds. In this study, 15 fungal species were reactivated in Petri dishes containing PDA medium. After reactivation, microfungal extracts 15 were obtained according to a methodology adapted Smedsgaar. The micro extracts were evaluated by UPLC / MS to determine the chromatographic profile and was made to evaluate the clusters separation main components, and are also subjected to antimicrobial assays and test preliminary cytotoxicity on Artemia salina for the assessment of biological potential. chemical studies were conducted with Aspergillus sp. EJC08, Exserohilum rostratum 1:11 Er and Aspergillus sp. FRIZ12. The three fungal species were grown on solid medium (rice) in sufficient quantity for the isolation of secondary metabolites. The compounds were isolated by chromatographic methods and identified by spectroscopic methods. Twelve compounds were obtained, three steroids in common fungal species (ergosterol, ergosterol peroxide and cerivisterol), mixture of two steroids commonly found in plants (β-sitosterol and estigmaterol), five substances polyketide source (2-benzyl- 4H- pyran-4-one, flavasperona, annularinas I and J monocerina), a carbohydrate (5- hydroxymethylfurfural) and a nucleoside (uridine). Of the isolated compounds, there are the annularinas I and J, novel natural products, and 2-benzyl-4H-pyran-4-one, second occurrence as a natural product. The polyketides and uridine showed good antimicrobial activity and moderate cytotoxic activities in general.