Navegando por Assunto "Biometria testicular"

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Acesso aberto (Open Access)
Biometria testicular, caracterização e congelamento de sêmen de macacos-de-cheiro de vida livre (Saimiri vanzolinii, S. cassiquiarensis e S. macrodon) e cativeiro (S. Collinsi) em água de coco em pó (ACP-118®)
(Universidade Federal do Pará, 2014-06-24) OLIVEIRA, Karol Guimarães; DOMINGUES, Sheyla Farhayldes Souza; http://lattes.cnpq.br/2794753357251149
Recently, Saimiri sciureus taxon (squirrel monkey) was revised and some subspecies are now classified by some authors as species, for example: Saimiri collinsi, S. cassiquiarensis and S. macrodon. Phenotypically these species are well determined. However, information on reproductive aspects is still quite scarce. The objective of the first phase of this study was to describe and correlate the testis with the seminal quality from captive (S. collinsi) and free-living (S. vanzolinii, S. cassiquiarensis and S. macrodon) squirrel monkeys. Length, width, height, circumference and testicular volume were measured as well as motility, vigour, plasma membrane integrity and morphology, for correlation data. No difference was observed between the right and left testicles within species, neither difference between species concerning to testicular measures. Semen, which was collected by electroejaculation with rectal probe, was constituted by a coagulated fraction, a liquid fraction or both. Seminal quality was similar among species. There is no correlation between testicular volume and seminal volume (of liquid or coagulated fraction) in S. collinsi and S. vanzolinii. The second phase of this work aimed to propose a semen freezing protocol in an extender based in ACP- 118® (powdered coconut water), testing two glycerol concentrations (1.5 and 3%) for captive S. collinsi. The third phase aimed to test a semen cooling protocol without addition of egg yolk (extracellular cryoprotector) followed by freezing with the best glycerol concentration observed in the preceding experiment and apply these protocols, previously tested in captive S. collinsi, in free-living males from S. vanzolinii, S. cassiquiarensis and S. macrodon in field conditions. Semen from S. collinsi could be cooled in ACP-118® without egg yolk. The freezing protocol developed proved to be useful for semen cryopreservation of S. Collinsi, although requiring improved to allow better maintenance of sperm quality after thawing. The procedure proved to be also applicable under field conditions on free-living animals, providing very encouraging results for the formation of genebanks for species vulnerable to extinction as the S. vanzolinii.
Acesso aberto (Open Access)
Ultrassonografia testicular, em machos bubalinos criados em regime extensivo no estado do Pará
(Universidade Federal do Pará, 2011-07-07) MANRIQUE AYALA, Henry Daniel; RIBEIRO, Haroldo Francisco Lobato; http://lattes.cnpq.br/1614582293203770
The objective was to verify the biometrics associated with testicular ultrasound as a tool in the selection of sexual precocity in male buffalo. We used 19 male crossbred Murrah and Mediterranean breeds, aged between 11 and 59 months, from May 6 to November 18, 2010. The animals were divided into 12 age groups: 8 animals from 12 to 19 months, 3 animals from 20 to 29 months, and 8 animals from 30 to 59 months. The animals were kept under extensive system in the low land to pasture canarana (Eriochloa SP) without mineralization. The animals were subjected to clinical andrological examination with semen collection by transrectal massage of the ampoules and physical analysis of the ejaculate. The sonographic examinations were performed with the device model Mindray DP-2200, 75L50EAV transrectal linear transducer, multi-frequency 5.0 / 7.5 / 10.0 MHz echodensity The testicular (Ecot) was expressed in number of pixels / area, using the program Image J. We collected blood samples to measure testosterone levels. Statistical analysis was performed using the SAS statistical software 1999. The results of scrotal circumference show averages and standard deviations minimum and maximum of 12.88 ± 0.51cm for animals of 12 and 13 months and 28.38 ± 0.38cm in males aged greater than or equal to 60 months, respectively. The testicular volume measurements showed minimum and maximum values of 30.28 ± 17.37cm³ in animals of 12 and 13 months and 534.25 ± 25.36 cm³ in animals greater than or equal to 60 months, respectively. The mean and standard deviation of the sperm concentration maximum and minimum found were 5.5 ± 3.5 (x 10⁶ / mm³) for animals of 16 and 17 months and 51.41 ± 2.26 (x 10⁶ / mm³) for greater than or equal to 60 months , respectively. Sperm motility in the minimum and maximum percentages were obtained 10% for animals with 16 and 17 months and 80 ± 5.77% to greater than or equal to 60 months, respectively. The total sperm defects found were minimum and maximum of 18.5% in animals of 16 and 17 months and 3%. in greater than or equal to 60 months, respectively. Testosterone levels were found minimum and maximum of 0,070 ± 0,026 ng / ml for 12 and 13 months and 2762 ± 0457 ng / ml for animals greater than or equal to 60 months, respectively. The echogenicity for animals aged 12 to 13 months was 78.67 ± 6.36 pixels, in the range of 14 and 15 months was 94.22 ± 3.40 pixels, for animals aged 16 and 17 months was 88.16 ± 3.95 pixels, animals between 18 and 19 months was 96.09 ± 3.40 pixels, for animals of 20 and 21 months was 103.12 ± 3.86 pixels, for 22 and 23 months was 98.4 ± 5.87 pixels, for the age of 24 to 29 months was 114.05 ± 2.42 of pixels, aged 30 to 35 months was 109.24 ± 3.13 pixel for animals of 36 at 41 months was 98.67 ± 5.3 pixel, between 42 to 47 months the average was 99.33 ± 2.1 pixel, between 48 to 59 months was 96.17 ± 1.90 pixel and animals greater than or equal to 60 months of age was 90.13 ± 1.77 pixel. We conclude that ultrasonography, the interpretation of the echogenicity, associated with the clinical andrological examination, is a tool that can be used in the selection of precocity and fertility evaluation of male buffalo.