Navegando por Assunto "Câncer - Tratamento"

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Acesso aberto (Open Access)
Análise do perfil do número de cópias e transcriptoma de pacientes com gliomas e em linhagens de glioblastomas tratadas com pisosterol
(Universidade Federal do Pará, 2018-10-17) FERREIRA, Wallax Augusto Silva; OLIVEIRA, Edivaldo Herculano Corrêa de; http://lattes.cnpq.br/0094007714707651
Central Nervous System Tumors (CNS) account for approximately 2% of all cancers. Although the incidence of CNS tumors is small, compared to other neoplasms, these tumors are among the most serious human malignancies because they affect the organ responsible for the coordination and integration of all organic activities. Gliomas represent approximately 80% of all intracranial tumors, typically affecting adults, with a high incidence between 40 and 65 years of age. Although numerous anti-glioma drugs have already been developed, they induce adverse reactions and their therapeutic effects are not satisfactory. The objective of this study was to evaluate and compare the profile of Copy Number Variation (CNV) and gene expression of patients diagnosed with gliomas and in glioblastomas cell lines (U87-MG, U343, AHOL1 and 1321N1) treated with pisoterol. For rhe experiments done with the cell lines treated with pisoterol, we demonstrated that they were highly sensitive to pisoterol treatment. This drug reduced the number of live cells in a dose-dependent manner. In addition, we demonstrated that after 48h of exposure to pisoterol, all cell lines were blocked in G2/M. Finally, we demonstrate that the pisosterol can modulate the expression of several genes of ATM/ATR pathway, promoting apoptosis. We demonstrated on genomic scale that all the cell lines had more genes that were significantly down-regulated than up-regulated after the treatment with pisosterol. For the experiments done with the gliomas biopsies, we demonstrated that only 11 genes (TNFRSF1A, SNAPC2, CASP8, IRAK3, GPX3, FZD9, TFAP2C, CDH1, RPRM, POU4F3 and MGMT) exhibited changes in the pattern of methylation in all grades analyzed. In addition, the methylation pattern of these 11 genes had correlations with some clinicopathological characteristics, such as age, sex and histological grade. And finally, we made a molecular characterization describing the CNVs of the gliomas originating from Belém-PA.
Acesso aberto (Open Access)
Perfil citopatológico de pacientes atendidas na Casa da Mulher e avaliação da atividade do ácido caurenoico contra linhagens de câncer cervical
(Universidade Federal do Pará, 2018-02-28) ROCHA, Silvia Maria Machado da; ROCHA, Carlos Alberto Machado da; http://lattes.cnpq.br/5789536737681588; BAHIA, Marcelo de Oliveira; http://lattes.cnpq.br/3219037174956649
Cervical cancer (CC), which is of great importance for public health, is considered to be the 4th most common type of cancer in the world. Human papillomavirus (HPV) infections are the main risk factor. Approximately 70% of women with CC are in less developed regions, demonstrating a socioeconomic relationship. Papillomavirus belong to the family Papillomaviridae, with more than 40 genera, of which five are composed of HPVs, with at least 200 species already described associated with human infections. These virus are enveloped, spherical and have a double-stranded DNA genome. HPVs have tropism by epithelial cells and persistent infections can lead to progressive intraepithelial transformations with evolution to precursor lesions of cervical cancer, and finally cancer. HPV infection is the most common sexually transmitted infection in the world, and most sexually active people, men and women, will have contact with the virus at some point in their lives. In the present study, the cytopathological profile of patients treated at a Secondary Reference Unit in Gynecological Cancer of the city of Belém / PA and the activity of caurenoic acid against cervical cancer lines were analyzed. The profile of the microbiological and cytopathological findings was verified in the examinations carried out by the Casa da Mulher laboratory during a period of one year, using data from 2,202 cervical cancer preventive exams (PCCU), showing a weak correlation between age range and frequency of microbiological findings and pathological alterations. The microbiological findings were the presence of three species: Gardnerella vaginalis (23.48%), Candida sp. (12.44%) and Trichomonas vaginalis (0.68%). The prevalence of cytologic abnormalities corresponded to 5.72%. Atypia cells of undetermined significance corresponded to 2.679%, and the total proportion of potentially malignant neoplastic lesions was 1.09%. The increase in PCCU coverage in the female population needs to be achieved and health promotion must be effected through intersectoral partnerships, popular participation, and collective accountability for quality of life. On the other hand, the evaluation of the genotoxic and mutagenic effect of caurenoic acid (CA) in cervical cancer lines was performed using HeLa (HPV18-positive), CaSki (HPV16-positive) and C33A (HPV-negative). AC showed a strong positive correlation with the genotoxicity indicators evaluated. At high concentrations, it inhibited the expression of E6 and E7 HPV genes, which interfere with cell cycle regulation. Although genotoxic effects were observed, the assays pointed to the possibility of using CA as feedstock for therapeutic agents against cervical cancer with HPV, as well as future research on E6 and E7 functions.