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Atividade antineoplásica da 1-desoxinojimiricina em modelos de câncer in vitro
(Universidade Federal do Pará, 2021-12) FONSECA, Suzanne Suely Santos da; PEREIRA JÚNIOR, Antônio; http://lattes.cnpq.br/1402289786010170; https://orcid.org/0000-0002-0808-1058
Cancer is one of the diseases that kill the most in Brazil, with alarming projections until 2030. In view of the problems related to cancer treatment, such as the compromise of healthy cells and, consequently, the presence of adverse effects, it is imperative to seek of alternative substances that may have antineoplastic effects and that are effective in the treatment of patients with this disease. In this way, the use of bioactive compounds has been widely used in the fight against neoplasms. Thus, the substance 1-deoxynojimyricin (1-DNJ) isolated from Bagassa guianensis may have great anticancer potential. In view of the problems related to cancer treatment, such as the impairment of healthy cells and, consequently adverse effects, it is necessary to search alternative substances that may have antineoplastic effects and they are effective in the treatment of patients with this disease. The objective of this study was to evaluate the effect of 1-deoxynojirimycin (1-DNJ) extracted from wood residue of the species Bagassa guianensis in different cancer cell lines to investigate possible antineoplastic actions in vitro using gastric adenocarcinoma and glioblastoma cancer cell lines. To do that, it was evaluated the effect of the substance 1-deoxynojirimycin on cell viability in vitro after 72h of treatment in cell lines ACP02 and A172. We also evaluated the effect of this bioactive compound on cell migration pattern, cell death by apoptosis and cell cycle changes using flow cytometry and reactive oxygen production. The results showed that 1-deoxynojirimycin makes a significant reduction in cell viability of cancer cell cultures in both glioblastoma (A172) and gastric cancer cell (ACP02) cell lines. The reduction in viability appears to be more effective in glioblastoma cell lines, with a common ic50 much lower when compared to other cell lines. We propose that the reduction in viability may be related to the decrease in reactive oxygen production in both lines after treatment with 1-DNJ. Besides that, 1-DNJ interrupts the cell cycle, prevents cell migration and induces necrosis-like cell death in the ACP02 lineage and apoptosis in the A172 lineage. Therefore, we suggest that 1-deoxynojirimycin may be an important and effective chemopreventive substance for the treatment of glioblastoma and gastric adenocarcinoma cancers.