Navegando por Assunto "Cromatografia líquida acoplada com detector de arranjo diodo"

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Acesso aberto (Open Access)
Desenvolvimento de um método para caracterização do extrato hidroetanólico das folhas de Mikania lindleyana DC por Cromatografia Líquida de Alta Eficiência (CLAE)
(Universidade Federal do Pará, 2011) OWITI, Alex Oselu; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070
Mikania lindleyana DC (Asteraceae), vernacular “Sucuriju”, is a creeping, shrubby, perennial, evergreen plant, growing in the Amazon region where it is used as diuretic, anti-hypertensive, anti-inflammatory and anti-ulcerous medicine. The main aim of this study was to develop a method for characterization of hydro-ethanolic extract (tincture) from the leaves of M. lindleyana DC, using UV and High performance Liquid Chromatography (HPLC). The hydro-ethanolic extract (tincture) was prepared based on Brazilian Pharmacopeia V, 2010 and its dry residue was submitted to phytochemical analyses, Thin Layer Chromatography (TLC) and High Performance Liquid chromatography (HPLC). Among the substances observed in the phytochemical analysis was coumarin, alkaloids, amino acids, reducing sugars, phenols, tannins, steroids, terpenes, saponins and organic acids. TLC test was done by applying hexanic, chloroformic and ethyl acetate Fractions together with Crude hydro-ethanolic extract and aqueous solution of coumarin Aldrich® 0,1mg/ml on the same plate using toluene/dichloromethane/acetone (45:25:30) as the eluent system. The chromatogram was then sprayed with an alcoholic solution of KOH 5%, and then observed under UV light at 254nm and 365 nm, revealing a light green band at an RF-0.61 for chloroformic fraction, crude hydro-ethanolic extract and for coumarin samples. The HPLC analysis was conducted using methanol/water 53:47 as the eluent system. The chloroformic fraction(CL), coumarin and the crude hydro-ethanolic extract (CHE) showed peaks at Retention time around 6.00minutes and spectra with maximum absorptions between 270nm and 300nm.This clearly demonstrate the presence of coumarins in the samples (CL= 0.209mg/mL and CHE= 0.014mg/mL).
Acesso aberto (Open Access)
Investigação fitoquímica biomonitorada da tintura 70% de Arrabidaea chica (Humb. & Bompl.) Verlot
(Universidade Federal do Pará, 2011) RIBEIRO, Jeane Francisca Alves; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070
The Arrabidaea chica (H & B) Verlot, popularly known as Pariri or crajirú. Scandentia is a small shrub about 2.5 m high, occurring from southern Mexico to central Brazil, is common in the Amazon, where it is used by Indians for body painting.Popularly tea leaves is indicated for inflammation, anemia, intestinal cramps, regulate menstruation, diarrhea, among others. Recent studies have shown its antioxidant activity and wound healing in vivo and in vitro, and antifungal activity against Trichophyton mentagrophytes and trypanocidal effect. This paper aims to contribute to the validation of the claim of use and to standardize the form of employment A. chica herbal medicine as a resource, since it is in RENISUS. The ethanolic and aqueous extracts of the plant were investigated for their secondary metabolites. Parameters were determined for quality control of the plant drug, through grading, drying powder loss and total ash.Besides the chromatographic profiles obtained by TLC, CLV, LC-MS and LC - DAD. The plant meets the parameters of quality control in accordance with the literature. A CCD profile obtained with the eluent Hexane / Acetone / MeOH (67,5:27,5:5) and revealed with NP / PEG revealed a band with Rf = 0.17 with yellow fluorescence characteristic of flavonoids. In the profile of HPLC - DAD chromatograms were obtained with characteristics of quinones and flavonoids as well. From these results, the work was directed to the isolation, initially with the FAE by fractionation of CLV and analysis of resulting fractions by HPLC-DAD, and as a result suggested the presence of a flavonoid apigenin derivative of the flavonol. The microbiological results corroborate some of its popular uses thus contributing to validate the form of alleged use of the plant.