Navegando por Assunto "Cromatografia líquida acoplada com espectômetro de massa"

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Acesso aberto (Open Access)
Avaliação da atividade antinociceptiva e anti-inflamatória de Mikania lindleyana DC: validação do uso na medicina popular
(Universidade Federal do Pará, 2011-06-20) SILVA, Andressa Santa Brigida da; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070; SOUSA, Pergentino José da Cunha; http://lattes.cnpq.br/9909053957915090
Mikania lindleyana DC. (Asteraceae), popularly named sucuriju, is a common plant in Brazilian Amazonia. Ethnopharmacological studies show a diversity of uses of this species such in treatment of gastritis, infection, pain and inflammation. To validate the claim form and use was decided to study the acute toxicity and the antinociceptive and anti-inflammatory activities in experimental animal models and the chemical composition of lyophilized crude aqueous extract of Mikania lindleyana (AEML). The phytochemical screening of AEML showed the presence of saponins, proteins, amino acids, phenols, tannins, organic acids and flavonoids. On the thin layer chromatography (TLC) blues fluorescence characteristics of the coumaric acid were identifieds. The analisis on high-performance liquid chromatography coupled to mass spectrometry (HPLC-DAD-MS) seems compounds highly glycosylated. The dose 5000mg/kg of EAML did not cause death in animals. The AEML, at doses 125, 250, 500, 750, 1000 and 1500mg/kg, significantly inhibited (in a dose-dependant way) the number of contortions induced by acetic acid on writhing test. The median effective dose (ED50) of 692.6 mg/kg did not prolong the latency-time in the hot plate. The AEML showed activity in both first and second phase of formalin-induced licking response decreasing of the lick paw of mice, where this effect was reversed by the opioid antagonist naloxone. The AEML was able to inhibite the erythema formation when compared to control group, but did not inhibite the paw edema formation induced by dextran. The AEML inhibited the paw edema carrageenan-induced from 2nd hour and reduced neutrophil migration to peritoneal cavity. Our results indicate that AEML demonstrate an antinociceptive effect with probable involvement of the opioid system, and anti-inflammatory activity through inhibition of prodution of inflammatory mediators as PGs, and also on adhesion molecules with involvement of cytokines.
Acesso aberto (Open Access)
Investigação fitoquímica biomonitorada da tintura 70% de Arrabidaea chica (Humb. & Bompl.) Verlot
(Universidade Federal do Pará, 2011) RIBEIRO, Jeane Francisca Alves; SILVA JÚNIOR, José Otávio Carréra; http://lattes.cnpq.br/4437885351749994; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070
The Arrabidaea chica (H & B) Verlot, popularly known as Pariri or crajirú. Scandentia is a small shrub about 2.5 m high, occurring from southern Mexico to central Brazil, is common in the Amazon, where it is used by Indians for body painting.Popularly tea leaves is indicated for inflammation, anemia, intestinal cramps, regulate menstruation, diarrhea, among others. Recent studies have shown its antioxidant activity and wound healing in vivo and in vitro, and antifungal activity against Trichophyton mentagrophytes and trypanocidal effect. This paper aims to contribute to the validation of the claim of use and to standardize the form of employment A. chica herbal medicine as a resource, since it is in RENISUS. The ethanolic and aqueous extracts of the plant were investigated for their secondary metabolites. Parameters were determined for quality control of the plant drug, through grading, drying powder loss and total ash.Besides the chromatographic profiles obtained by TLC, CLV, LC-MS and LC - DAD. The plant meets the parameters of quality control in accordance with the literature. A CCD profile obtained with the eluent Hexane / Acetone / MeOH (67,5:27,5:5) and revealed with NP / PEG revealed a band with Rf = 0.17 with yellow fluorescence characteristic of flavonoids. In the profile of HPLC - DAD chromatograms were obtained with characteristics of quinones and flavonoids as well. From these results, the work was directed to the isolation, initially with the FAE by fractionation of CLV and analysis of resulting fractions by HPLC-DAD, and as a result suggested the presence of a flavonoid apigenin derivative of the flavonol. The microbiological results corroborate some of its popular uses thus contributing to validate the form of alleged use of the plant.