Navegando por Assunto "Enriquecimento ambiental"

Agora exibindo 1 - 4 de 4

Acesso aberto (Open Access)
Alterações da morfologia da micróglia do septo lateral e comportamento semelhante ao ansioso em um modelo murino de inoculação sequencial de VDEN1 e VDEN4: influência do enriquecimento ambiental
(Universidade Federal do Pará, 2016-05-05) GOMES, Giovanni Freitas; DINIZ, Cristovam Wanderley Picanço; http://lattes.cnpq.br/2014918752636286; SÓSTHENES, Márcia Consentino Kronka; http://lattes.cnpq.br/7881527576747420
Dengue disease is the major cause of deaths by arbovirus infections in Brazil. In the American Continent, the epidemics seem to be associated to the fact that multiple dengue virus (VDEN) serotypes circulate simultaneously. Despite its epidemiological importance and a century of systematic studies dedicated to understand the disease, its detailed pathogenic mechanisms remain poorly understood. The objective of this study was to evaluate possible influence of environmental enrichment on behavioral changes and microglial morphology alterations in the lateral septum after sequential VDEN1 and VDEN4 intraperitoneal inoculations of infected brain homogenates. To that end, we used adult females ten months old of an immunocompetent albino Swiss mouse strain housed in standard or enriched cages. A single intraperitoneal infection of VDEN1 was followed after 28 days by another inoculation of VDEN4. To enhance clinical signs, a regimen of daily alternated injections of VDEN1 or VDEN4 followed 24 hours later by anti-VDEN2 antibody was applied in the last 7 days. Control animals received equal volumes and regime of inoculation of uninfected brain homogenate. We assessed the behavioral changes using the open field exploratory (OF) and elevated plus-maze (EPM). Infected animals housed in standard cages showed significant decrease in time of exploration of the periphery in the OF and in the time of exploration of enclosed arm in the EPM. Uninfected mice housed in standard cages and animal housed in enriched cages did not show same changes. To check how possible microglial changes could be influenced by acute DENV1 infection, secondary DENV4 infection or the passive anti-DENV4 inoculation, we decided to sacrifice groups of animals after which point of inoculation. To evaluate microglial changes, we did selective immunohistochemistry for microglia and macrophages using anti-IBA-1 antibody (Wako, Japan) and we used tri-dimensional reconstruction to morphometric evaluation. Compared to uninfected, infected mice from standard cages showed significant changes in microglial morphology. We also tested the hypothesis that septal microglia is clustered in subtypes and that DENV infection could change this pattern. We noticed microglia is subdivided in three subgroups in physiological conditions, a more complex pattern, a less complex pattern and an intermediate. After DENV1 or DENV4 infection, we observed changes in this pattern, including the appearance of a high complexity cell, increasing the percentage of complexes microglia. We observed these changes in animals from standard cages, but not in animals from enriched cages. Another interesting data is that environmental enrichment appears to reduce this morphometric changes. Based on the evidences, we suggest that sequential infection with VDEN1/VDEN4 in murine model induced behavioral changes and microglial changes in the lateral septum and EA appears to protect animals against these alterations. Based on these evidences, we suggest that microglial from lateral septum present a heterogeneous pattern of morphology and that DENV infection can induce morphological changes, and alterations in the pattern of subdivision, associated with the increase in the percentage of high complexity cells. In addition, infection can induces behavioral changes detected by EPM and OF tests and environmental enrichment seems to protect against microglial and behavioral changes.
Acesso aberto (Open Access)
Bem estar em cativeiro: análise e planejamento da ocupação do tempo em macacos-prego (Cebus apella)
(Universidade Federal do Pará, 2009) LESSA, Miguel Angelo Monteiro; GALVÃO, Olavo de Faria; http://lattes.cnpq.br/7483948147827075
Foraging is one of the most important activities and consuming great part of the day time of neotropical primates in the natural environment. In the wild, Capuchins spend up to 80% of their time traveling and foraging in the search for food. On the other side, in captivity no effort is usually required to obtain food. Therefore the opportunity to express foraging activities becomes virtually excluded in captivity. The main objective of this work was to create and evaluate the effectiveness of an environmental enrichment tool called “bowlboard”, designed to difficult access, extending the time devoted to reach food. The observation was carried through in four different contexts, being one in the morning and three in the afternoon, using a focal animal sampling method. The relative duration of each behavioral event was compared in the absence and presence of the enrichment. It was found that the bowller extended foraging time, functioning as instrument of environmental enrichment. A considerable lowering in frequency of abnormal behaviors, while search and manipulation of food was more time consuming.
Acesso aberto (Open Access)
Bem-estar de macacos-prego no cativeiro: engenharia comportamental no enriquecimento ambiental e análise da dinâmica espacial.
(Universidade Federal do Pará, 2014-03-25) LESSA, Miguel Angelo Monteiro; GALVÃO, Olavo de Faria; http://lattes.cnpq.br/7483948147827075; https://orcid.org/0000-0001-9912-3833
Capuchin monkeys (Sapajus ssp.) in captivity have few opportunities to perform characteristics skills of these monkeys in the natural environment. The responsibility for the welfare of the capuchin monkeys living in the Experimental School for Primates poses the task of finding healthier living conditions. The objective is to build captive environment conditions functionally similar to the natural environment, using physical, foraging, cognitive and social environmental-enrichment techniques. Aditionally, interested in knowing about the social relations within the spatial interactions it is proposed to describe the relationships in terms of inter-individual spatial proximity and preferred heights in different groups of Sapajus spp. kept in captivity. Fourteen individuals of four groups of three institutions were observed in three different contexts: before, during and after feeding. The positions of each individual were recorded using the scan sampling method. The results showed a pattern of inter-distance between different dyads of animals in study groups. The monkeys were closer after feeding and far before and during feeding. Differences among preferred places were also observed. The dominant monkeys spent more time in places that were most attractive. The subordinate monkeys spent more time on the opposite and less attractive places in relation to the place occupied by the dominant. The overall pattern of interindividual spacing was similar to that observed in nature, male capuchin monkeys showed a characteristic avoidance and no approximation.
Acesso aberto (Open Access)
Enriquecimento alimentar e cognitivo para o bem-estar em cativeiro
(Universidade Federal do Pará, 2012) COUTINHO, Paulo Henrique Módena; GALVÃO, Olavo de Faria; http://lattes.cnpq.br/7483948147827075
When put an animal in captivity, we limit their actions, taking control of most of the variables that act on a restricted environment. These variables, although to remedy the basic needs of the animals has the potential to be highly predictable and therefore stress. Environmental enrichment can be one of the most important advances in animal health in captivity in recent times, raising the psychological and physiological parameters of well-being, suppressed by captivity. The main objective was to create and verify the effectiveness of an environmental enrichment equipment called "roulette" with the function of minimizing the harmful effects of the two captive capuchins, by increasing the time devoted to foraging activities, approaching its budget activities relevant to that of animals in the wild. The observations were made by instantaneous sampling method in five steps: 1) pre-intervention, 2) D5, 3) D10 4) D15 and 5) post-intervention, and subsequently compared longitudinally. The data demonstrate the effectiveness of roulette item enriching the environment, since the behaviors associated with the exploitation and displacement suffered increased over the intervention - with the exception of D10, which will soon be re-evaluated - keeping average frequency of foraging near than expected in free-living animals (Rímoli, 2001) still in the post-intervention observations, rather than the frequency of abnormal behavior or stereotyped.