Navegando por Assunto "Enzymes"

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Acesso aberto (Open Access)
Estudo de misturas de enzimas (complexo celulásico, complexo enzimático, xilanase, β-glucanase e xilanase, β-glucosidase e Glucoamilase) na bioconversão do bagaço da cana-de-açúcar em etanol
(Universidade Federal do Pará, 2015) MOREIRA, Rosiane Fernandes; MACHADO, Nelio Teixeira; http://lattes.cnpq.br/5698208558551065
This work, it was proposed to evaluate mixtures of commercial enzymes by supplier Novozymes A / S. The enzymes used in this work were: celulase complex, xylanase, β-glucosidase, enzymático complex, xylanase and β-glucanase and glucoamylase in the glucose production from sugarcane bagasse subjected to treatment with alkali hydroxide solution sodium at room temperature, 70 ° C, 90 ° C and 120 ° C. The BCA yield on a dry basis after treatment with NaOH solution 6 (w / v) were 30.64% ± 1.395 (PACTA), 44.00% ± 1.787 (PAC70), 65.91% ± 1.096 (PAC90), and 95.25% ± 1.461 (CAP 120), respectively. The ash content for the BCA were 2.05% ± 0.027 (PACTA), 0.62% ± 0.013 (PAC70), 0.48% ± 0.007 (PAC90) and 0.18% ± 0.008 (PAC120). The lignin contents were 20.67 ± 0.603 (PACTA), 13.03 ± 0.711 (PAC70), 6.05 ± 0.196 (PAC90) and 5.49 ± 0.151 (PAC120). The results suggest that the conversion rates of cellulosic waste into glucose are strongly dependent on temperature in the alkaline pulping process. The kinetic parameters obtained in kinetic adjustments enzymatic hydrolysis of the BCA for PACTA, PAC70, PAC90 and PAC120 were: Vmax (g/h) equal to 7.20; 5.12; 4.54 and 0.87 respectively; Km (g) equal to 3.6; 2.56; 2.27 and 2.56 respectively; Kcat (h) equal to 1.44; 1.02; 0.91 and 0.17 respectively; Km/Vmax equal to 0.5 for all samples and Kcat/Km of 0.4 for all samples.
Acesso aberto (Open Access)
Uso de processo enzimático usando a obtenção de carotenoides da torta residual de dendê
(Universidade Federal do Pará, 2017-02-22) MESQUITA, Eric César Mano; CORRÊA, Nádia Cristina Fernandes; http://lattes.cnpq.br/5763999772352165
The residual fibers from the production of palm oil are a potential source of carotenoids of great economic importance, particularly β-carotene, due to its biological characteristics in human health and its pro-vitamin A. The enzymes cellulite have been shown to be a strong alternative to increase the yield in the oil extraction process, used particularly for the pretreatment of the constituent material of the plants, as it favors the release of the oil inside the plant cell, contributing to the increase of the amount of oil to be extracted and still reducing the extraction time, thus providing the efficiency of the extraction processes of compounds of industrial interest. In the pressed mesocarp pie (TMD), a pre-treatment with the enzymes cellulase (CELLUCLAST) and pectinase (PECTINEX) at the times of 3.6, 12, 24, 48 and 60 h was carried out to evaluate the highest volume yield carotenoid degradation. Then, the effect of the addition of the lipase enzyme was evaluated through a rotational central composite experimental design 2³, on the quality of the oil, in terms of β-carotenes (μg /gTMD48). The results for the pre-treated TMD indicate that 6 hours would be enough to reach this goal, obtaining 55% mass of carotenoids. For the results with addition of lipase, it was observed that in the highest concentration there was a decrease in acidity and an increase in the content of carotenoids. In the present study it was observed that hydrolysis did not occur as a function of the amount of water in the reaction medium favoring the opposite reaction, the esterification of the free fatty acids.