Navegando por Assunto "Felinos"

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Acesso aberto (Open Access)
Antibodies to Leishmania spp. in domestic felines
(Universidade Federal do Pará, 2015-12) OLIVEIRA, Gabriela Capriogli; PAIZ, Laís Moraes; MENOZZI, Benedito Donizete; LIMA, Michele de Souza; MORAES, Carla Cristina Guimarães de; LANGONI, Helio
Leishmaniasis is a vector-borne zoonotic disease caused by protozoa in the genus Leishmania, typical of rural and peri-urban environments. The causative agent of American visceral leishmaniasis is Leishmania (Leishmania) infantum chagasi and the main insect vector in Brazil is Lutzomyia longipalpis. Dogs (Canis familiaris) are important in the transmission of the disease, as a reservoir closely related to humans and an infection source for phlebotomine vectors. Since 1990, an increasing number of feline leishmaniasis cases have been reported, suggesting that domestic cats (Felis catus) might be involved in the epidemiology of the disease. The present study analyzed the prevalence of anti-Leishmania spp. antibodies in naturally infected domestic cats from various neighborhoods in the municipality of Belém, Pará, Brazil, using the indirect immunofluorescence assay (IFA) and the direct agglutination test (DAT). Among the 443 samples tested, 18 (4.06%) presented positive reactions in the IFA. The observed titers were 40 IU in 4.97% of the samples and 80 IU in 0.90%. In the DAT test, positive results were found in 25 (5.64%) of the samples. The observed titers were also 40 IU (4.97%) and 80 IU (0.68%). The agreement rate between the two tests was considered low (Kappa coefficient = 0.10).
Acesso aberto (Open Access)
Colonic epithelial nodular hyperplasia associated with strongyloidiasis in cats in the Amazon region, Pará State, Brazil
(Universidade Federal do Pará, 2017) MOURA, Márcio Alan Oliveira; JORGE, Ediene Moura; NASCIMENTO, Kelly Karoline Gomes do; CORREA, Gabriela Riet; ABEL, Isis; CAVALCANTE, Gustavo Góes; OLIVEIRA, Carlos Alberto de; BEZERRA JÚNIOR, Pedro Soares
Strongyloides spp. are intestinal parasites that affect several animal species. Four species of the genus have been reported in domestic cats: S. felis, S. planiceps, S.stercoralis and S. tumefaciens . Reports describing infection by these nematodes in domestic cats in Brazil are scarce. This study aimed to describe the pathological features of Strongyloides spp. parasitism in two cats in the Amazon region, state of Pará, Brazil. During the necropsy of the two cats, numerous whitish nodules approximately 0.2cm in diameter were observed in the wall of the large intestine. The nodules were conspicuous in the colonic mucosa and exhibited a punctate aperture facing the center of the lumen. Microscopically, these nodules were formed by projections of the mucosal epithelium into the submucosa, which formed tubules lined with a single layer of columnar epithelium, with high cellularity and rare goblet cells, characterizing epithelial hyperplasia of the crypts. Adult nematodes and eggs observed in the lumen of the tubules were morphologically compatible with Strongyloides spp. Numerous larvae were also observed in the interstitium adjacent to the nodule. A mild lymphocytic infiltrate was observed neighboring the hyperplastic nodules. The histological changes are consistent with those described for S. tumefaciens infection.
Acesso aberto (Open Access)
Ocorrência de micoplasmas hemotrópicos em caninos e felinos domésticos na região de Belém, PA
(Universidade Federal do Pará, 2014-02-07) MORAES, Leopoldo Augusto; GONÇALVES, Evonnildo Costa; http://lattes.cnpq.br/8652560763793265; PEREIRA, Washington Luiz Assunção; http://lattes.cnpq.br/9266281009742277
Hemotrophic mycoplasmas comprise a group of bacteria that can cause hemolytic anemia and several chronic diseases in animals. In cats three infecting species of hemoplasmas are reported: M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, the first being considered the most pathogenic, whereas the dogs are normally infected by at least two mycoplasma species M. haemocanis and ‘Candidatus M. haematoparvum’. In order to assess the occurrence of hemotrophic mycoplasmas in feline and canine populations of Belém city were analyzed, by PCR, the genetic material from blood samples of 299 domiciled and stray animals. In 8.03% (24/299) of DNA samples of hemotrophic mycoplasmas was amplified, 1.78 % (3 /169) from infected dogs and 16.15% (21 /130) from infected cats. Among the cats the highest rates of infection were found in domiciled animals 22.86% (16/70) and in males 29.51% (18/61), the species ‘Candidatus M. haemominutum’ 8.46 % (11/130) get the higher incidence when compared to species Mycoplasma haemofelis 6.92 % (9/130). The infection in dogs was only identified among stray animals, with the ‘Candidatus M. haematoparvum’ being the only species identified with a rate of 1.78 % (3/169). It was possible to assess that hemotrophic mycoplasmas circulate in both species in the present report, domiciled felines and males formed the group at highest risk for infection and the agent ‘Candidatus M. haematoparvum’ seems to be the only one in circulation in the canine population.
Acesso aberto (Open Access)
Pesquisa de anticorpos anti-Toxoplasma gondii (Nicolle & Manceaux, 1909) em felídeos selvagens nos municípios de Capitão Poço e Belém, Pará
(Universidade Federal do Pará, 2008-02-29) SILVA, Carolina Costa; SOUZA, Nazaré Fonseca de; http://lattes.cnpq.br/1616332835810182
Toxoplasmosis is one of the most widespread zoonosis in the world caused by Toxoplasma gondii, a protozoan that has in felids its unique and definitive host. Twenty one animals of four species were avaluated, Jaguarundi (Herpailurus yaguarondi), Ocelot (Leopardus pardalis), Margay (Leopardus wiedii), Jaguars (Panthera onca) to investigate the occurrence of toxoplasmosis in two municipalities of Pará State, using two serological assays, indirect hemagglutination, (IHA), direct modified agglutination (MAT) and coproparasitologic examination. From the tested animals, 18 were positive (85,72%). Twelve (57,14%) animals by the IHA and 14 (66,66%) by the MAT, were seropositive. In both utilized techniques there were no statistical differences between seropositivity and the genus. In the Herpailurus genus 4,6% of seropositivity was found in both tests; in the Leopardus 19,05% by IHA and 28,57% by MAT; and in the Panthera 33,33% in both techniques. Similar results were verified in 11 animals. Comparing both techniques, there were no statistical differences. Higher titers were obtained from a margay (1024) by MAT. No oocysts of T. gondii were found in the studied animals. It was verified high occurrence of toxoplasmosis in the studied areas and both techniques are efficient for serologic diagnoses of this disease.
Acesso aberto (Open Access)
Swimmer syndrome affecting an entire litter of kittens
(2013-06) CARDILLI, Diogo José; JOÃO, Carolina Franchi; VOORWALD, Fabiana Azevedo; FARIA, Joice Lara Maia; COSTA, Mirela Tinucci; TONIOLLO, Gilson Hélio
Swimmer syndrome is a developmental abnormality characterized by a delay in the ability to walk and move about and is manifested in newborns between 15 and 20 days of age. These animals exhibit constant coxofemoral articulation abduction, and their pelvic limbs move caudally and laterally. This case reports a litter of three crossbreeds kittens (26 days old) with swimmer syndrome. Each animal was treated with physical therapy and shackle bandages that kept their pelvic limbs bent and close to the body. After seven days, all of the animals exhibited normal ambulation, indicating that this treatment had a curative effect on the kittens.
Acesso aberto (Open Access)
Vitrificação de tecido ovariano de gata doméstica (Felis catus): um modelo para a preservação da fertilidade em felinos silvestres
(Universidade Federal do Pará, 2016-09-06) BRITO, Danielle Cristina Calado de; SANTOS, Regiane Rodrigues dos; http://lattes.cnpq.br/0500967766886604; PIECZARKA, Julio Cesar; http://lattes.cnpq.br/6644368250823351
The aim o the present thesis was to develop an efficient vitrification protocol of the ovarian tissue from domestic cat (Felis catus). This study was divided: Phase I: Effect of different basis media during the vitrification of cat ovarian tissue; Phase II: Effect of different sugars (extracellular cryoprotectants) and the vitrification technique for the vitrification of feline ovarian tissue. In phase I, the morphology of preantral follicles was similar (p > 0.05) to fresh control when RPMI-1640 was used as basis medium for vitrification. RPMI-1640 does not contain phenol red, which was found to enhance ethylene glycol (EG) toxicity during vitrification. In phase 2, the percentage of morphologically normal preantral follicles was similar (p > 0.05) to fresh control only when the vitrification medium contained 0.1 or 0.5 M trehalose, instead of sucrose or raffinose at same concentrations. Furthermore, based on parameters such as morphology, cell proliferation and thickness of collagen fibers, it is possibe to assume that efficient vitrification of feline ovarian tissue can be performed by combining trehalose with EG, with or without dimethylsulfoxide (DMSO), applying the solid-surface vitrification (SSV) or ovarian tissue cryosystem (OTC) method. Although vitrification with OTC in the presence of EG did not differ from the other treatments, this protocol presented the highest percentages of preserved preantral follicles (56%), being similar to control (64%). Additionally, no effect on gene regulation was observed after vitrification when apoptosis markers (BAX – protein X associated to Bcl-2), endoplasmic reticulum (ER) stress (ER protein 29 – ERP29), water channels proteins like aquaporins 3 and 9 (AQP3 and AQP9), the membrane ABC transporters ABCB1 and ABCG2, except when the SSV method was applied using only EG as cryoprotectant followed by seven days in vitro culture, where ERP29 up-regulation (ER stress) and AQP9 down-regulation (impaired water transport) were observed. Based on this, it can be concluded that to efficiently preserve feline ovarian tissue, is is necessary the use of a vitrification protocol free of phenol red, supplemented with trehalose, as extracellular cryoprotectant, and EG alone or in combination with DMSO, as intracellular cryoprotectants. Both open (SSV) and closed (OTC) systems are equaly efficient to maintain follicular survival during the vitrification procedure.