Navegando por Assunto "Gastrite"

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Acesso aberto (Open Access)
Efeitos do consumo de água de pH alcalino em pacientes com gastrite e correlação com marcadores epigenéticos relacionados com a inflamação
(Universidade Federal do Pará, 2018-10-10) CHAVES, Juliana Ramos; KHAYAT, André Salim; http://lattes.cnpq.br/6305099258051586
In the carcinogenesis of gastric cancer, the stages usually manifest clinically as gastritis, gastric atrophy, ulcerations, intestinal metaplasia, dysplasia and finally, as malignant neoplasia. The association between gastric cancer and diet is already widely described in the literature and several studies have demonstrated the influence of food intake with preservatives and with high concentration of nitrates and salt, with the development of this neoplasia. Regarding water consumption, there’s no relevant evidences. The pH of most of the water sold in the metropolitan área of Belem does not match the standarts recommended by the govermnment Health department, being more acidic. Thus, the benefits of both healthy eating and alkaline water consumption are object of several discussions. Nowadays the other types of markers that can aid the detection of pre-neoplastic and neoplastic lesions will be hosted. Among them, find themselves as epigenetic proteins. Environmental factors such as diet, inflammation and infection have been excluded as contributors to epigenetic changes. Hence, the present work intends to provide evidence that only the water pH modification is able to lead the variations the expressions pattern of miRNAs, associated with a first stage of gastric carcinogenesis, a gastritis. For this it was applied the microRNAs miR-7, mir-155, mir-29c and mir-135b, in 28 patients porters of gastritis that were burned to digestive endoscopy alkaline PH. After collection, the RNA from the samples was extracted, and the complementary tape DNA (cDNA) was obtained. The cDNAs were submitted to qPCR amplification analysis for analysis of miRNA expression. They assessment were using the Biostat and Stata 11.0 programs, being statistically superior to values of p <0.05. Comparing the levels of expression and clinical evaluation of gastritis by EDA before and after alkaline water consumption, the results demonstrated that there was a increasing of the target microRNAs, of miR-7 (p = 0.09), miR155 (p = 0.13), miR-29c (p =0.21) and miR-135b(p=0.19). On the other hand, it was possible to observe a significant endoscopic improvement of the gastritis (p=0.024), demonstrating the clinical benefit of alkaline water intake.
Acesso aberto (Open Access)
Estudo do perfil de pacientes submetidos a pesquisa de Helicobacter pylori: análise endoscópica e dos fatores determinantes da atividade linfocitária na resposta imunológica gástrica (ROR-Y, FOXP3 e GATA3)
(Universidade Federal do Pará, 2015) MIRANDA, Ariney Costa de; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
INTRODUCTION: Helicobacter pylori is known for its adaptability to the host may progress to chronic infection using diverse and effective mechanisms of pathogenicity. It has high worldwide incidence and its direct relationship with peptic ulcer, gastritis, gastric carcinoma and lymphoma occurs in a minority of infected individuals. A better understanding of the genetic regulation of gastric immune response, motivated this investigation. OBJECTIVES: Describe the transcription factors of T lymphocytes positive for ROR-γ, FOXP3 and GATA3, correlating them with the intensity, type and degree of activity of gastritis, caused by H. pylori infection METHODS: The study included 50 patients of both sexes who underwent upper gastrointestinal endoscopy with biopsy. Urease test and histology were made for identification and confirmation of infection by the bacteria. Thirty-five samples were sent to the immunopathology laboratory NMT-UFPA to study gene expression of transcription factors of T lymphocytes (ROR- γ, FOXP3 and GATA3) by RT-PCR method. RESULTS: We obtained 48.5% positive H. pylori urease test in patients and 25.7% positive of H. pylori in the histological study. The confirmation of H. pylori held by these two exams was 11.7%. In this sample, 22% of individuals reported having a previous Helicobacter pylori infection. The age and gender of the individuals did not influence the gene expression of the studied factors. The H. pylori positive individuals showed a higher expression of the GATA3 gene with prior infection report, and mild erosive gastritis body classified by the Sydney system via endoscopy. The ROR-γ gene presented with increased expression only when comparing samples with or without positive for H. pylori (histology), by the topography of the inflammatory process evidenced by endoscopy. The terms of the factors in the study were more significant when we used the β-actin gene as standard when compared to the GAPDH gene. CONCLUSIONS: The adult age group analyzed in our sample did not influence the gene expression of the studied transcription factors. 2- There were not found differences in the genes expressions that were studied, related to gender of the sample. 3- There was a significant gene expression not only in the patients that were H. pylori positive (histology), but also in the ones who reported previous infection in our study. 4-To compare the endoscopic findings of the sample using the Sydney system with the gene expression of transcription factors under study, we obtained better agreement only in the degree of activity of gastritis. 5- The transcription factor GATA3 (TH2 response profile) was the highest gene expression in samples with endoscopic gastritis and tested positive for H. pylori. 6- The transcription factor ROR-γ (TH17 response profile) presented with increased expression when comparing samples with the topography of the inflammatory process evidenced by endoscopy, regardless of positive H. pylori (histology). 7- The gene β-actin gene as a constituent standard used in our study was that showed significant results in quantified terms, when compared to the GAPDH gene.
Acesso aberto (Open Access)
Expressão dos antígenos ABH e Lewis na gastrite crônica e alterações pré- neoplásica da mucosa gástrica
(2002-12) AGUIAR, Délia Cristina Figueira; CORVELO, Tereza Cristina de Oliveira; ARAÚJO, Marialva Tereza Ferreira de; CRUZ, Ermelinda do Rosário Moutinho da; DAIBES, Samiry; ASSUMPÇÃO, Mônica Baraúna de
BACKGROUND:The major cause for chronic gastritis in human is the infection by the Helicobacter pylori. The blood group antigens present at the gastric mucous are described as possible receptor for this bacteria in the epithelium. The alterations in the expression of blood group patterns are associated with the development of gastric cancer. OBJECTIVES: Verify the H. pylori prevalence and examine the immunohistochemical distribution of the ABH and Lewis antigens expression to correlate with histopathological alterations. PATIENTS AND METHODS: From 63 chronic gastritis patients were investigated gastric biopsies, blood and saliva samples by dot-blot-ELISA, indirect immunoperoxidase and hematoxylin-eosin and Gram. RESULTS: No significant association between the presence of the bacteria and the ABH, Lewis and Secretor phenotype was found. For the majority of the patients the antigen expression of the ABH and Lewis blood group was restricted mainly to the foveola epithelium of the gastric mucosa, similar to the saliva. The inappropriate expression of these antigens occurred always in the presence of H. pylori and/or preneoplastic alterations of the gastric mucosa. In areas with intestinal metaplasias we also observed reduced reactivity for the H and Leb antigens and mainly the induced expression of Lea. CONCLUSION: Alterations in the pattern of the glycosylation of this antigens are interesting, because they reflect different stages in the cellular differentiation and become potential markers in the diagnostic evaluation and prognosis of gastric pathologies.