Navegando por Assunto "Healing"

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Acesso aberto (Open Access)
Avaliação da atividade cicatrizante do extrato hidroalcoólico de Ayapana triplinervis (Vahl) R.M. King & H. Robinson (ASTERACEAE)
(Universidade Federal do Pará, 2020-07-16) NASCIMENTO, Suellen Carolina Martins do; LIMA, Anderson Bentes de; http://lattes.cnpq.br/3455183793812931; https://orcid.org/ 0000-0002-0534-2654; ANDRADE, Marcieni Ataíde de; http://lattes.cnpq.br/8514584872100128; https://orcid.org/ 0000-0001-5875-695X
Wound healing is a complex process that involves the organization of cells, chemical signals and remodeling in order to repair injured tissue. North American statistical data show a prevalence of skin lesions in approximately 22.8% of the world's population. In Brazil, wounds are a serious public health problem. In this sense, the use of medicinal plants as therapeutic agents has aroused interest among researchers due to their most different effects, including healing. Thus, ethnopharmacological studies are found in the literature, which associate the use of A. triplinervis with wound healing, but there is no scientific evidence to prove this activity. Thus, this study aims to evaluate the healing potential of the hydroalcoholic extract of A. triplinervis incorporated in an ointment of 5 and 10%. For that, phytochemical analyzes were carried out: prospecting of the plant drug and the hydroalcoholic extract, by means of colorimetric, precipitation tests, thin layer chromatography and high-performance liquid chromatography of the hydroalcoholic extract. In addition to the macroscopic, morphometric analysis, and histological examination of the hydroalcoholic extract ointment in 5% and 10%, using Dersani® and saline solution, respectively, as positive and negative controls. Through these tests, it was observed that the plant drug presented several compounds: saponins, anthraquinones, steroids and triterpenes, polyphenols and coumarins. For the hydroalcoholic extract the result was similar, but the tests did not indicate the presence of anthraquinones. Analysis by thin layer chromatography of the hydroalcoholic extract revealed positive results for coumarins, steroids and triterpenes. In the chromatographic profile, the extract, obtained by high performance liquid chromatography, showed a peak, being suggestive of coumarin. Macroscopic analysis of the lesions showed that the groups of the 5% and 10% extract ointments had more re-epithelialized wounds. In morphometry there was no statistical difference between the four test groups in the percentage of wound contraction. However, the histological examination showed that the ointment with 10% hydroalcoholic extract showed better quality in the development of tissue repair, as it increased fibroblasts, collagen, keratinization, more than the other groups. Thus, this study showed that A. triplinervis extract ointment did not accelerate the speed of wound closure, however, it did show a beneficial influence on the quality in which the lesions evolved, yet further research is necessary to better understand It is made.
Acesso aberto (Open Access)
Métodos de obtenção e caracterização de biomembrana de quitosana e copaíba para potencial uso em feridas
(Universidade Federal do Pará, 2022-06-14) PARANHOS, Sheila Barbosa; PASSOS, Marcele Fonseca; http://lattes.cnpq.br/0588450144351187; https://orcid.org/0000-0002-5616-2127; CANDIDO, Verônica Scarpini; http://lattes.cnpq.br/8274665115727809; https://orcid.org/0000-0002-3926-0403
Health professionals deal directly with several complex situations in the care of sick people. Among these, there are skin wounds that can harm the patient's clinical condition, in addition to costly treatment for healing. Skin wounds require dressings to protect against pathogenic microorganisms and to accelerate the healing process. With the emergence of biomaterials available for use in wound treatment, chitosan has become an effective choice, easily found in a natural and renewable form with healing potential. The chitosan membrane presents ideal conditions in the treatment of wounds, such as absorption, protection, biocompatibility and antimicrobial potential. To increase its healing effects, natural oils have been incorporated into the polymer matrix, such as copaiba, which has a high anti-inflammatory action. In this context, the work aimed to obtain and characterize chitosan membranes by emulsion and nanoemulsion of copaiba oil to treat skin wounds. The chitosan membranes with oil addition by emulsion and nanoemulsion were synthesized by the solvent evaporation technique. They were evaluated by macroscopic analysis and characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, differential scanning calorimetry, swelling percentage, humidity, contact angle. An in vitro assay of antibacterial activity against the bacterium S.aureus was carried out. The membranes had an apparently thin appearance, little malleability, relative opacity, continuous and good handling. He observed more porosity on the surfaces of membranes synthesized by nanoemulsion of copaiba oil, in addition to a more amorphous behavior. He noticed a better interaction between chitosan and oil constituents when the oil synthesis was prepared by nanoemulsion, resulting in improved stability of the material produced. The swelling percentages were higher in the MQCoN-0.1 (214±3.22%) compositions when immersed in water and the MQCoN-5.0 composition (220±6.83%) in the PBS solution. The wetter behavior was significant in membranes composed of 0.1% (0.80±1.37%) and 0.5% (3.00 ±0.79%) copaiba oil nanoemulsions. There was no great influence on the contact angle between syntheses and compositions. The chitosan membrane with 1.0% (v/v) of emulsified oil and the chitosan membrane with 0.5% (v/v) of nanoemulsified oil were the most hydrophilic membranes. All membranes were able to inhibit bacterial growth, except the chitosan membrane with 1.0%(v/v) oil emulsion. Materials obtained by nanoemulsion have ideal attributes for application in the use of skin wounds.
Acesso aberto (Open Access)
Síntese e Caracterização de Membrana de Quitosana com Extrato de Banana Verde e Andiroba para Cicatrização de Lesões Epiteliais
(Universidade Federal do Pará, 2022-03-21) FERREIRA, Elisângela da Silva; CANDIDO, Verônica Scarpini; http://lattes.cnpq.br/8274665115727809; https://orcid.org/0000-0002-3926-0403
Biomaterials must enable the surrounging healthy cells to grow and replace the matrix that constitutes the material. The application of other bioactives is being widely studied, as well as the incorporation of phytosan into chemical removal films in wound healing by different techniques. This study aimed to synthesize and characterize chitosan membranes, green banana peel extract and andiroba oil for application in epithelial devices. Membranes were made in different compositions and characterization tests were carried out on the synthesizer. Andiroba oil was added in pure form or oil-in-water (O/W) emulsion in the chitosan solution. Higher in chitosan membranes with emulsion O/W, being 978.79 % e 423.64 %, in Nano M6 and Nano M11, respectively, after 24 hours of life in water. The highest percentage of moisture was in M7 (24.9 ± 3.8 %) and the lowest in Nano M7 (13.46 ± 0.8 %). All samples are hydrophilic, with lower values with banana peel extracts from the first decoction, M1 (24.0° ± 1.9°) and Nano M6 (23.7° ± 4.3°). Diffractograms are mostly amorphous material, for the most part. Thermal analysis has greater stability of chitosan membranes synthesized with banana peel extract and addition of andiroba oil. Spectroscopy showed characteristic peaks and bands of sample components and interaction between these components, as well as suggestion or encapsulation of the oil. The material first presented characteristics of absorption of liquids and synthesized, in particular the membranes of banana extraction of the day of decoction interaction and with addition of O/W emulsions, with greater possibility of duration of its healing of composting epithelial deficiencies.