Navegando por Assunto "Hematologia"

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Análise crítica dos achados hematológicos e sorológicos de pacientes com suspeita de Dengue
(2008-10) BARROS, Lilian Patrícia Souza; IGAWA, Sônia E. S.; JOCUNDO, Susana Y.; BRITO JUNIOR, Lacy Cardoso de
The increase in the number of more serious forms of dengue fever among cases in the city of Belém, Brazil has astounded local authorities. The objective of this study was to conduct a critical analysis of hematologic and serologic findings of patients with clinical suspicion of dengue seen at a clinical laboratory in Belém in Pará State. This retrospective study involved 210 patients who were referred to the Laboratory of Clinical Pathology Dr. Paulo C. Azevedo in Belém, in the period of February and March of 2007, with requests for a complete blood count and serological test for IgM to confirm the diagnosis of dengue. Of the cases studied, 51/210 (24.3%) presented with thrombocytopenia and 53/210 (25.2%) leukopenia. The serologic test for IgM was positive in 47.1% (99/210) of cases. A statistically significant association (p<0.05) was observed only among patients who presented with thrombocytopenia (33/99) and positive serology for dengue, suggesting that the hematological alterations of leukopenia and thrombocytopenia, frequently associated with this illness, may not be present at the beginning of the infection. It is therefore essential to carry out a serologic test for IgM to confirm a diagnosis of dengue.
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Hematologia como ferramenta no monitoramento do "status" da cadeia produtiva de oito espécies de Acaris ornamentais (Loricariidae) do médio Rio Guamá, estado do Pará
(Universidade Federal do Pará, 2011-02-28) NEVES, Mikaelle de Souza; FUJIMOTO, Rodrigo Yudi; http://lattes.cnpq.br/9538142371454660
The present study monitored the health of eight species of ornamental plecos (acaris) captured and marketed in the Guamá River Middle - Pará, through the establishment of the hematological baseline value, evaluation of transport stress and infestation by Trypanosoma spp. The ornamental plecos were: ancistrus (Ancistrus sp. - L338), loricaia (Rineloricaia lanceolata - L10), picoto (Hypostomus sp. - L28), bola (Peckoltia oligospila - L06) pleco (CochiIodon sp. - L145), canoa (Lasiancistrus saetiger - L323), assacu (Pseudacanthicus spinosus - L160) and pinima (Leporacanthicus galaxias - L07).The blood collections for determination of hematological baseline (Chapters I and II) were realized at the site of capture fish, under the least stress as possible. Blood samples uninfected were separated to enable comparisons with the others after the transport stress (Chapters III and IV) and also with fish infected by Trypanosoma spp. (Chapter V). The transport stress lasted 3h, in density 1,5 fish/L, simulating the marketing process the fish in the region and was avalued et 0, 6, 24, 48, 72 and 96h after stress. In Chapter I and II, the hematological baseline showed significant difference (p> 0.05) among the seven species of plecos although they belong to the same family and share similar ecological niches. The transport stress for 3h (Chapters III and IV) not compromise the health of plecos, since most of the hematological parameters returned to baseline within 24h for the bola, 48h for the pleco and 72h for the picoto, being respectively, the minimum time indicated by the results of these fish to maintained before a new trade. The all eight plecos species studied were infected by Trypanosoma spp. (Chapter V). Normocytic-hypochromic anemia was observed in ancistrus and canoa, and hypochromic-macrocytic anemia in loricaia. In infected pinima showed under stress with lymphocytopenia, neutrophilia and monocytosis. Therefore, the results of this test provided an assessment of the healthiness of these species of ornamental plecos through the haematological examinations, thereby subsidize the development or adequacy of management less stressful for these plecos in order to assist the sustainability of ornamental plecos extractive chain.
Acesso aberto (Open Access)
Hematological and morphometric differences of blood cells from rheas, Rhea americana (Struthioniformes: Rheidae) on two conservation farms
(Universidade Federal do Pará, 2017-01) GALLO, Samira Salim Mello; EDERLI, Nicole Brand; OLIVEIRA, Francisco Carlos Rodrigues de
Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espírito Santo (ES), and the other in São Carlos, São Paulo (SP), were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained. The results from the two farms analyzed were compared by means of the t test. There were differences in the parameters of the erythrocyte series between the two flocks because of a process of hypochromic macrocytic anemia observed among the rheas in ES. The values for the erythrocyte series and indexes were: RBC, 2.81 ± 0.15 x106/μl; PCV, 44.20 ± 2.86%; Hb, 12.12 ± 0.74 g/dL; MCV, 15.75 ± 0.89 fL; MCH, 43.18 ± 1.82 pg; and MCHC, 27.44 ± 0.80 g/dL. The values for the leukocyte series were: WBC, 12,072 ± 4116 /μL; heterophils, 64.10 ± 9.90%; eosinophils, 2.05 ± 2.06%; monocytes, 6.40 ± 2.99%; lymphocytes, 26.93 ± 9.62%; and basophils, 0.52 ± 1.27%. These can all be suggested as references for rheas reared in Brazil. Statistical differences were observed in erythrocyte length measurements, which were higher in the rheas in SP than in those in ES. Regarding width, the heterophils, lymphocytes and monocytes of the birds in ES were statistically greater than those of the birds in SP. It was concluded that rheas may exhibit morphometric alterations to blood cells and differences in blood elements according to the type of management to which they are subjected.
Acesso aberto (Open Access)
Hematological, morphological and morphometric characteristics of blood cells from rhea, Rhea Americana (Struthioniformes: Rheidae): a standard for Brazilian birds
(Universidade Federal do Pará, 2015-11) GALLO, Samira Salim Mello; EDERLI, Nicole Brand; BÔA-MORTE, Murilo de Oliveira; OLIVEIRA, Francisco Carlos Rodrigues de
Blood exams are an indispensable tool in bird medicine. This study aimed at describing values and aspects of rheas' hematology, Rhea americana, as well as analyzing the morphology and morphometry of all blood cells. Fifty eight adult rheas of both sexes from two farms, one in Cachoeiro de Itapemirim, Espírito Santo State and the other in São Carlos, São Paulo State, were selected. Blood samples were taken and RBC count, PCV and Hb levels measured and used in hematimetric indexes calculations. The total and differentiated leukocyte counts, as well as the TPP and fibrinogen were determined. Erythrocytes, leukocytes and thrombocytes were identified and characterized morphologically. The values for the red series and hematimetric indexes were: RBC (2.81±0.15×106/μL), PCV (44.20±2.86%), Hb (12.12±0.74 g/dL), MCV (15.75±0.89 fL), MCH (43.18±1.82 pg), MCHC (27.44±0.80 g/dL); the values of white series were: WBC (12.072±4116/μL), heterophils (64.10±9.90%), eosinophils (2.05±2.06%), monocytes (6.40±2.99%), lymphocytes (26.93±9.62%), basophils (0.52±1.27%). One may conclude that on average, rheas' blood cells are larger than those of other birds, but these cells in smears cannot be differentiated only by their size. Besides rheas' leukocytes have different components and coloring as in other bird species, however, there are no components or staining aspects unique to the species.
Acesso aberto (Open Access)
Importância da presença de granulações tóxicas para o diagnóstico hematológico de septicemia
(2007-12) SALGADO, Danielle Nazaré da Silva; CARVALHO, Raimundo Gladson Corrêa; OLIVEIRA, Maria de Fátima Pinto; SANTOS, Eduardo José Melo dos; BRITO JUNIOR, Lacy Cardoso de
This work aims at investigating the association of the presence of toxic granulation with positive blood cultures, age of patients, conditions of hospitalization and types of bacterial agents. Blind prospective and retrospective, analyses were carried out for the presence of toxic granulations-in blood samples of 300 patients of the both genders hospitalized in the City of Belém, Pará, Brazil. Request blood tests over a two year period were evaluated. The blood tests and cultures were performes using automated methods. All the data were statistically compared using the Qui-square test (clump method). The results show statistical associations between: (1) the presence of toxic granulations and positive blood cultures; (2) lower ages of patients (the newborn) and positive blood cultures; (3) hospitalization in the ICU and positive blood cultures and (4) toxic granulations-and the observation of leucocytosis and right-left shunts in patients hospitalized in the ICU with positive blood cultures. The commonest bacterial agents identified were klebsiella oxytoca (22%), Acinetobacter calcoaceticus (20%), Escherichia coli (18%), Enterobacter cloacae (14%), and Pseudomonas aeruginosa (8%).
Acesso aberto (Open Access)
Intervalos de referência sanguíneos e a influência da idade e sexo sobre parâmetros hematológicos e bioquímicos de ovinos da raça Santa Inês criados na Amazônia Oriental
(Instituto Nacional de Pesquisas da Amazônia, 2015-09) LIMA, Misael Brito de; MONTEIRO, Maria Vivina Barros; JORGE, Ediene Moura; CAMPELLO, Claudio Cabral; RODRIGUES, Luiz Fernando Souza; VIANA, Rinaldo Batista; MONTEIRO, Frederico Ozanan Barros; COSTA, Cícero Temístocles Coutinho
Blood count and biochemical tests are used to diagnose diseases in domestic animals. These tests can be influenced by age, gender, nutrition, breed, species and environmental conditions. Thus, data from one region should not be extrapolated to animals raised in other regions. The objective of this study was to determine the hematological and biochemical values of different ages and genders of Santa Inês sheep, raised in the eastern Amazon. There were examined 91 sheep that were assigned to three groups: G1 (three to six months old, n = 31); G2 (seven to 24 months old, n = 30) and G3 (above 24 months old, n = 30). The blood cell count, white blood count and biochemical determinations were made with an automatic counter and a semi-automatic analyzer, respectively. Mean values were compared using the Tukey test. The number of erythrocytes, the red blood indices, the thrombogram, eosinophils, total protein, urea and creatinine concentrations were influenced by the age of the animals. The erythrocyte coefficient of variation and the creatinine concentration were influenced by gender, and were greater in males. The neutrophil:lymphocyte (N:L) ratio was greater than one for all age groups. This study led to the determination of reference values for sheep raised in the Eastern Amazon and demonstrated that when interpreting hematological and biochemical tests of sheep, age and gender must be considered.
Acesso aberto (Open Access)
Perfil hematológico de búfalas da raça Murrah, criadas ao sol e à sombra, em clima tropical da Amazônia Oriental
(Instituto Nacional de Pesquisas da Amazônia, 2011) SILVA, Jamile Andréa Rodrigues da; ARAÚJO, Airton Alencar; LOURENÇO JÚNIOR, José de Brito; VIANA, Rinaldo Batista; SANTOS, Núbia de Fátima Alves; GARCIA, Alexandre Rossetto
We evaluated the hematologic responses of 20 female buffaloes raised under full sun (group NS) and shade (group SG) at Belem, Para, Brazil. The animals in the SG group (n = 10) were maintained in paddocks under a silvopastoral system with Racosperma mangium, and the ones in the NS (n = 10), in paddocks with Brachiaria humidicola, with drinking water and mineral salt, but without access to shade. Air temperature (AT), relative air humidity (RH) and black global temperature (BGT) were measured for each treatment. Blood sampling for erythrogram and leukocyte count were undertaken at intervals of 14 days, and carried out at 1:00 p.m., during 2009. AT and black global temperature and humidity index (BGHI) were different (P < 0.05) throughout the year, and their highest values were observed in the NS group. During the rainier period, the SG group presented the highest values of leukocytes (P < 0.05), although in the transition and low rainy periods, the highest values were observed in the NS group. In the low rainy period, the erythrocytes count showed the highest values in the NS group. The highest levels of hemoglobin (P < 0.05) were observed during the transition and low rainy periods. Only hemoglobin presented a significant and negative (P < 0.05) correlation with RH. We concluded that the female Murrah buffaloes are subject to a hostile environment, and that the low rainy period is the most favorable for causing heat stress.
Acesso aberto (Open Access)
Perfil hematológico e bioquímico de búfalos ( Bubalus bubalis ) na Amazônia Oriental
(Universidade Federal do Pará, 2014-12) FONTES, Deiseane Gaia; MONTEIRO, Maria Vivina Barros; JORGE, Ediene Moura; OLIVEIRA, Carlos Magno Chaves; RITTER, Rhuan Amorim; BARBOSA NETO, José Diomedes; SILVA FILHO, Ednaldo da; MONTEIRO, Frederico Ozanan Barros
Complete blood cell count and biochemical testing are exams routinely used in assessing the health of domestic animals, including buffaloes. In the Amazon region, research on this subject is scarce. Thus, the aim of this study was to establish reference intervals for hematology and blood chemistry of Bubalus bubalis raised in eastern Amazon and evaluate the effects of age and sex on biochemical and hematological values. Seventy-three (n=73) Murrah buffaloes were divided into three groups, group 1 (G1, n=22): animals from two to eight months, group 2 (G2, n=23): animals from nine months to two years and group 3 (G3, n=28): animals over two years. Blood counts and biochemical analyzes were performed on automated equipment. The reference intervals were established as recommended by Clinical and Laboratory Standards Institute (CLSI). Tukey and Kruskal-Wallis tests were used to evaluate the effect of gender and age, with differences considered significant when P <0.05. There was influence of age on the values of red blood cells (RBC), packed cell volume (PCV), hemoglobin , leukocytes, lymphocytes, eosinophils, neutrophils, platelets, mean platelet volume (MPV), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (HCM), Red Blood Cell Distribution Width (RDW) and the proportion of neutrophil: lymphocyte (N:L). The gender affected MCV and the Platelets Distribution Width (PDW), which were higher (P<0.05) in females, while RDW was higher in males. Comparing the biochemical parameters among age groups, it was found that age influenced the activity of the enzymes aspartate aminotransferase (AST) and alkaline phosphatase (ALP) and the concentrations of creatinine, total protein and direct bilirubin. Creatinine concentrations and direct bilirubin were significantly higher in animals of older age. The gender affected the activity of AST and the concentration of direct bilirubin, which were higher (P <0.05) in males. Hematological and biochemical values set in this study can be used as a reference for buffaloes bred in eastern Amazon.