Navegando por Assunto "Hepatite C"

Agora exibindo 1 - 20 de 27

Acesso aberto (Open Access)
Análise de polimorfismo na região promotora do gene da Interleucina 18 (-137 G/C e -607 C/A) em pacientes portadores do vírus da hepatite C de Belém, Pará
(Universidade Federal do Pará, 2012-03-23) SANTOS, Kemper Nunes dos; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769
Since its discovery in 1989, the hepatitis C virus (HCV) has been recognized as a major cause of chronic liver disease worldwide. Considered a public health problem worldwide involving between 170 to 350 million people infected. Host genetic factors have been implicated in the persistence of HCV infection. Studies suggest that two single nucleotide polymorphisms at position -607 C/A (rs1946518) and -137 G/C (rs187238) in the region of the gene IL-18 and have been found associated with the transcriptional promoter activity of IL -18, and potentially of IFN-γ, being associated with delayed viral clearance and persistence of the disease. We conducted a cross-sectional analytical study of the city of Belém-PA in 152 blood samples from patients infected with HCV and 188 uninfected controls. The samples were subjected to RT-PCR (Reverse Transcriptase - PCR) for detection of viral RNA and, subsequently, the PCR-RFLP (Restriction Fragment Length Polymorphism) to evaluate the polymorphism in the promoter region of IL-18 gene at positions -137 G/C and -607 C/A. The results showed no significant difference for IL-18 polymorphisms between patients and control group. But showed a significant difference for homozygous genotypes G/G (39.1%) at position -137 (OR = 3.00, CI [95%] = 1.24 – 7.22, p = 0.02), and A/A (21.7 %), position -607 (OR = 3.62, CI [95%] = 1.25 – 10.45, p = 0.03) among women than men (22.6% and 7.6%). The results showed evidence that among women, the presence of the polymorphism homozygous A/A (-607) acts as a protective factor against HCV infection, genotype as the A/A (-607) have been linked in some studies with liver disease and mild viral clearance.
Acesso aberto (Open Access)
Analysis of polymorphisms in the interleukin 18 gene promotor (-137 g/c and -607 c/a) in patients infected with hepatitis c virus from the brazilian amazon
(Universidade Federal do Pará, 2015-09) SANTOS, Kemper Nunes dos; ALMEIDA, Marcella Kelly Costa de; FECURY, Amanda Alves; COSTA, Carlos Araújo da; MARTINS, Luisa Caricio
Background - The hepatitis C virus has been recognized as the leading cause of chronic liver disease in the world. Host genetic factors have been implicated in the persistence of hepatitis C virus infection. Single nucleotide polymorphisms at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the IL-18 gene promoter have been suggested to be associated with delayed hepatitis C virus clearance and persistence of the disease. Objective - Identify these polymorphisms in a population infected with hepatitis C virus from the Brazilian Amazon region. Methods - In a cross-sectional analytical study conducted in Belém, Pará, Brazil, 304 patients infected with hepatitis C virus were divided into two groups: group A, patients with persistent infection; group B, patients with spontaneous clearance. The control group consisted of 376 volunteers not infected with hepatitis C virus. Samples were analyzed by RT-PCR for the detection of viral RNA and by RFLP-PCR to evaluate the presence of the -137 G/C and -607 C/A IL-18 gene promoter polymorphisms. Results - Comparison of polymorphism allele frequencies between the patient and control groups showed a higher frequency of allele C at position -607 among patients (P=0.02). When the association between the polymorphisms and viral infection was analyzed, patients carrying genotype C/A at position -607 were found to be at higher risk of persistent hepatitis C virus infection (P=0.03). Conclusion - the present results suggest a possible role of the -607 IL-18 gene promoter polymorphism in the pathogenesis of hepatitis C virus infection.
Acesso aberto (Open Access)
Aspectos epidemiológicos da infecção pelo vírus da hepatite C em populações ribeirinhas do estado do Pará, na Amazônia brasileira
(Universidade Federal do Pará, 2015-09-24) FECURY, Amanda Alves; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769
The hepatitis C virus (HCV) has large number of infected and may cause hepatocellular carcinoma. Its genetic material RNA presents six different genotypes (1, 2, 3, 4, 5 and 6) and subtypes, related by disease prognosis and response to treatment. The main route of transmission of HCV is the parenteral route, but the vertical and intrafamily transmission and is also reported. Drug use and needlestick share were characterized as risk factors for acquisition of infection and pathways are described and confirmed by literature. Less is known about viral transmission in remote or hard to reach communities such as riverside communities. This study aimed to determine the prevalence of viral infection, evaluate the serological profile and circulating genotype HCV, and trace the epidemiological characteristics, describing the main risk factors for acquisition of HCV infection that different riverside communities living in the State of Pará, in the Eastern Amazon, are exposed. The respondents answered a questionnaire to obtain epidemiologic information and blood samples were collected to carry out the serological, molecular, and genotyping tests. 1.277 samples were collected from Surrounding of the Tucuruí Hydroeletric, Pacuí, Hole of Maracujá and Nazário communities. Predominant age group 18-37 years old, female, married, low education, fishermen or açaí extractor, with up to 1 minimum wage. Respecting the particular characteristics of each local, consumption of alcohol and tobacco ranged between communities, as well as sharing the spiked material and cutting, the manicure kits, surgeries, hospitalization, presence of sexually transmitted diseases and if has any familiar with hepatitis. The Pacuí presented prevalence of HCV infection of 8,84 % having moderate prevalence of infection and Tucuruí was 2,25 %, close to the national average. The genotype 1 was found in 100% of Pacuí samples and 70% of Tucuruí samples, which possessed the remaining 30% of the samples belonging to genotype 3, following the pattern expected genotype distribution. In Maracujá Hole and Nazário Hole, there were no positive samples. The communities seem to have individuals who should be considered for the taking of public health measures, with increased risk of infection through drug use and the sharing of needlestick objects. The viral circulation have intermediate to moderate prevalence, with standard genotypic distribution similar to the Brazilian. The distribution of the infection and its features can vary within the country and even within their own regions, depending on the presence and viral circulation, in addition to the risk factors to which comunities are exposed.
Acesso aberto (Open Access)
Associação do vírus da Hepatite C com o grupo sanguíneo ABO e Rh(D) em doadores de sangue da Fundação Hemopa, Pará, Brasil
(Universidade Federal do Pará, 2012-06-25) NÓBREGA, Letícia de Souza; CRESCENTE, José Ângelo Barletta; http://lattes.cnpq.br/5243773796185944
Several studies have demonstrated association of blood group systems with different pathologies. Therefore, this work aimed to investigate the association between HCV and blood groups ABO and Rh blood donors Hemopa Foundation from 2000 to 2010. Between January 2000 and December 2010 were analyzed 565,614 samples from individuals who donated blood in Hemopa, and 1064 showed these test reagent for HCV serologic survey, 1246 inconclusive and 563.304 negative. However, this study included only those blood donors who did not have any kind of co-infection to any other pathology researched, naturally Pará and brown skin. After the selection of these criteria led to a sample size of 838 (0.15%) seropositive in 1181 (0.21%) inconclusive and 551,991 (99.64%) seronegative for a total of 554.010 blood donors. For serological diagnosis of hepatitis C were used serological test ELISA (enzyme immunoassay) 4th generation for detection of specific anti-HCV in the serum of donors and the samples positive by serology (ELISA) was carried out extraction of viral RNA. The ABO and Rh blood groups were determined by the research of antigens A and / or B, anti-A and / or anti-B antibodies and Rh (D) antigens in the blood sample from the individual. Over the years we found that the seroprevalence of HCV-specific antibodies in blood donors has undergone reduction, ranging from 132 to 38 cases reactive serum. Most donors were between the age group 30-49 years (57.64%), belonging to the predominantly male 74.5% (624/838) and coming from Belém 72.5% (607/838). When the distribution of the phenotypes of the ABO and Rh blood groups systems between the donor reactive serum and non-reactive to HCV, it was found that no significant variation in the frequency of these phenotypes donor reagents and reactants in the non-HCV-specific antibodies. Donor blood PCR results showed that when compared to Rh and ABO blood groups of patients there was no association between them.
Acesso aberto (Open Access)
Association of killer cell immunoglobulin-like receptor polymorphisms with chronic hepatitis C and responses to therapy in Brazil
(2013) VASCONCELOS, Janaina Mota de; MÓIA, Lizomar de Jesus Maués Pereira; AMARAL, Ivanete do Socorro Abraçado; MIRANDA, Esther Castello Branco Mello; TAKESHITA, Louise Yukari; OLIVEIRA, Layanna Freitas de; MENDES, Lilian de Araújo Melo; SASTRE, Danuta; TAMEGÃO-LOPES, Bruna Pedroso; PEDROZA, Larysse Santa Rosa de Aquino; SANTOS, Sidney Emanuel Batista dos; SOARES, Manoel do Carmo Pereira; ARAÚJO, Marialva Tereza Ferreira de; BANDEIRA, Camila Lucas; SILVA, Adriana Maria Paixão de Sousa da; MEDEIROS, Zilene Lameira de; SENA, Leonardo dos Santos; DEMACHKI, Sâmia; SANTOS, Eduardo José Melo dos
Soroprevalence for Hepatitis C virus is reported as 2.12% in Northern Brazil, with about 50% of the patients exhibiting a sustained virological response (SVR). Aiming to associate polymorphisms in Killer Cell Immunoglobulin-like Receptors (KIR) with chronic hepatitis C and therapy responses we investigated 125 chronic patients and 345 controls. Additionally, 48 ancestry markers were genotyped to control for population stratification. The frequency of the KIR2DL2 and KIR2DL2+HLA-CAsp80 gene and ligand was higher in chronic infected patients than in controls (p < 0.0009, OR = 3.4; p = 0.001, OR = 3.45). In fact, KIR2DL3 is a weaker inhibitor of NK activity than KIR2DL2, which could explain the association of KIR2DL2 with chronic infection. Moreover, KIR2DS2 and KIR2DS2+HLA-CAsp80 (p < 0.0001, OR = 2.51; p = 0.0084, OR = 2.62) and KIR2DS3 (p < 0.0001; OR = 2.57) were associated with chronic infection, independently from KIR2DL2. No differences in ancestry composition were observed between control and patients, even with respect to therapy response groups. The allelic profile KIR2DL2/KIR2DS2/KIR2DS3 was associated with the chronic hepatitis C (p < 0.0001; OR = 3). Furthermore, the patients also showed a higher mean number of activating genes and a lower frequency of the homozygous AA profile, which is likely secondary to the association with non-AA and/or activating genes. In addition, the KIR2DS5 allele was associated with SVR (p = 0.0261; OR = 0.184).The ancestry analysis of samples ruled out any effects of population substructuring and did not evidence interethnic differences in therapy response, as suggested in previous studies.
Acesso aberto (Open Access)
Autoanticorpos contra antígenos celulares e sua correlação com o genótipo viral em pacientes com infecção pelo vírus da Hepatite C (HCV)
(Universidade Federal do Pará, 2011) SOUZA, Ana Maria Almeida; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
Inflammation of the liver caused by hepatotropic viruses affect millions of people and represents a significant public health problem worldwide. There are interactions between hepatotropic viruses and the host immune system that can influence the pathogenesis of liver injury. The objective of this study was to investigate the frequency of autoantibodies in patients with hepatitis C virus, and its correlation with the genotypes found. We studied 51 patients diagnosed by PCR of infection with hepatitis C and a group of 100 blood donors with all serological tests for infectious diseases negative. The 51 patients with virus C had an average age of 43 years, + / - 11.3, in the pre-treatment, 34 (66.7%) were male and 17 (33.3%) were female. Of these 13 (25.5%) were ANA positive, 45 (88.2%) were with genotype 1 and 11.8% with genotype 3. Patients who presented with antibodies had no detectable levels of AST, ALT, AST / ALT, γ-GT and alkaline phosphatase significantly different from those with negative antibody titers. Thus, we conclude that the antibodies present in the study sample are independent of disease progression and patient prognosis, though seems to be linked with genotype 1.
Acesso aberto (Open Access)
Carcinoma hepatocelular e as infecções pelos vírus das hepatites B e C na Amazônia Oriental: estudo clínico-sorológico e de biologia molecular
(Universidade Federal do Pará, 1999-08-06) MIRANDA, Esther Castello Branco Mello; BENSABATH, Gilberta; http://lattes.cnpq.br/0818254462635138; CRUZ, Ermelinda do Rosário Moutinho da; http://lattes.cnpq.br/6061358867607317
In order to contribute to a better understanding of the possible role of hepatitis B and C in the etiopathogenis of hepatocellular carcínoma (HCC) in the East Amazon, there were studied 36 patients admited in three public hospitais in Belém/PA, from January,1992 to March,1999. The criteria to be enrolled in the study were the association of clinical and image procedures, alpha-fetoprotein (AFP) above 400nglml in the serum and/or histopathology examination compatible to hepatocellular carcinoma. Clinical aspects, biochemical and histological examination, serum levei of AFP and serological B and C hepatitis markers were evaluated. Polymerase chain reaction assays were used to detect serum virus nucleic acids, HBV DNA and HCV RNA. There was a predominance for male sex in the prevalence of HCC (p<0.01 ) in a ratio of 6.2 to 1 (male/female). Regarding age, the Mean and the Median were 50.8 and 53.0 years respectively, and the Amptitude was 6 to 81 years. Most patients (52.7%) carne from rural regions. Farmer was the main occupation referred (p<0.01). Alcohol abuse was observed in 33.3%. Abdominal pain and hepatomegaly were the most recorded sign and symptoms in 94.4% of patients. Cirrhosis was detected in 83.3%. The majority of it belonged to class B or class C of Child-Pugh classification. During diagnosis, 50% of patients had some kind of complication, specially portal-systemic encephalopathy and gastro-intestinal hemorrhage (upper digestive bleeding) related to the originai chronic liver disease. In 88,9% of the sample, one or more hepatitis B markers were positive. Also, 8,3% those patients had anti-HCV simultaneously positive. There were no serological markers in 11,1% of the casuistic. 58.3% had HBsAg serological positive test. Coinfection was detected in 2.8%. Antibodies to HBc were positive in 86%, being present in 25% of cases in association with the anti-HBs. Among patients positive to HBsAg, anti-HBe was observed in 85.7%, anti-HBe in 9.5% and IgM anti-HBc in 57.1%. There were no positive anti-HD in any sample case. The HBV DNA was found in 37.7% of ali patients and in 65% of the HBsAg positive. The HCV RNA was detected in 8.5% of the sample but in 100% of the patients positive to anti-HCV. Sera from patients negative to HBsAg or to anti- HCV had any evidence of HBV DNA or HCV RNA. The AFP was above the normal value in 88.9% of patients, with levels up to 400ng/ml in 75% of them and in 27.8% high titers superior to 70000 ng/ml were observed. The abdominal ultrasonography identified liver tumors with nodules, multiple or single, present respectively in 63.9% and 36.1% of the sample. The predominant histological tumor was trabecular well differentiated carcinoma (p<0.05). The least Mean age and the highest AFP serum levei (p<0.01 ) were recorded among patients HBsAg posítive. Most of patients had advanced illness. A death rate of 38.9% were recorded. In conclusion, hepatitis B virus infection seems to be important in the etiology of HCC in the East Amazon. Improving preventive measures such immunization and screening for early diagnosis of liver tumors in the risk population should be emphasyzed. Further control or direct studies need to be done to elucidate important possible cofactors in the region that may contribute to a better understanding of the mechanisms enrolled in hepatocarcinogenesis.
Acesso aberto (Open Access)
Co-infecção vírus da imunodeficiência humana e vírus da hepatite C (HIV/HCV): aspectos epidemiológicos, clínicos e laboratoriais de uma população atendida em um serviço de hepatopatias na cidade de Belém-Pará
(Universidade Federal do Pará, 2006) AMARAL, Ivanete do Socorro Abraçado; SOUZA, Rita Catarina Medeiros de; http://lattes.cnpq.br/3560941703812539
The Human immunodeficiency virus 1 (HIV-1) and Hepatitis C virus (HCV) infection currently appears as co-morbidity, which can intervene mainly in natural history of hepatitis C. Describing demographic, clinical and laboratorial aspects including histopathological examination, was the objective of this study. Between august of 2004 to december of 2006, 36 co-infected patients were selected for this study. 92% were from Belém, with 42 years old medium age; 72.52% singles; 83.5% male and 61.1% heterosexuals. Among possible risk factors for HCV, 41, 7% referred injectable illicit drug use, 38,9% intranasal cocaine and 38,9% syringe share. History of alcoholism (77,8%) and TARV use had been the possible factors for hepatic illness aggravations. A patient showed clinical signals of hepatic failure from chronic disease. Among biochemical hepatic tests, medium ALT and AST levels had been 68UI/L and 61UI/L, respectively. T CD4+ lymphocytes medium levels were 327cells/mm3. Medium HIV viral load was 2,53 log10 copies/mL (ep=0,34). Medium HCV viral load was 5,90log10UI/mL. HCV genotype 1 was the most frequent (58,82%). 57% of the patients submitted to liver biopsy presented fibrosis ranging from moderate to severe and 11% did not presented fibrosis by METAVIR classification. There was association between T CD4+ lymphocytes and ALT or AST levels (p=0,0009 and p=0,0002), and there was association between HCV genotype 1 and HCV-RNA viral load higher or equal to 6 Log10 (p=0,34). There was association between HCV-RNA and HIV-RNA (p=0,039) viral load. The patients presented good health conditions, no signs of liver failure and immunological stability, but showed important liver structure alterations. Therefore, they are good candidates for HCV antiviral therapy. Perhaps, future studies using controlled group, having a large casuistry are necessary for better understanding of HIV/HCV co-infection.
Acesso aberto (Open Access)
Correlação entre autoimunidade tireoidiana e o vírus da hepatite C
(Universidade Federal do Pará, 2012) ARAGÃO, Andrea Gomes de; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
The viral hepatitis are the most important subject of public health, being caused by differents etiologic agents. Amongst these, we find the hepatitis C virus (HCV) that according to the World Health Organization (WHO) its affects about 123 millions of people world-wide, a prevalence of 2%. The main form of transmission is the exposition to infected blood. The HCV is part of Flaviviridae family, Hepacivirus genus, its possess 6 genotypes and multiples subtypes. In Brazil, the genotype 1 is observed in 70% of infected patients, followed by genotype 3 (25%) and genotype 2 (5%). Some risk factors are strongly related with the HCV transmission, amongst them: the utilization of sterilized glass syringe at home; the sharing of utensils of personal hygiene as shavers, tooth brush, pliers of manicure and cutting nails and; blood transfusion before 1993. The present study aimed to execute an epidemiologic inquiry about risk factors related with the HCV infection and determinate the anti-HCV seroprevalence in candidates of blood donation, in the State of Pará. The following risk factors were: the use of sterilized needles and syringes at home; the use of proper material of manicure and pedicure; the use of disposable blades in public environment; the accomplishment of invasive dental treatment and; the act of receiving blood transfusion before 1993. The purpose the investigate the frequency of the autoantibodies of Brazilian hepatitis C virus carriers. The target group was represented by 14 HCV patients, while 45 healthy blood donors (HI) were included as controls. The following autobodies (Ab) to thyroid peroxidase (TPO –Ab) and anti-thyroglobulin (Tg – Ab) were investigated were investigated by indirect agglutination. In relation to the TPO – Ab, the bearers of HVC were negative in and in control group 92.86% were found positive and 15.56% 84.44% negative. As the Tg-Ab was positive in HCV carriers 92.86% 88.37% in the control group and. This study found no difference in the frequency of tireoidianas disorders when compared with hepatitis C patients without specific treatment with those without hepatitis C, probably justified, by the high prevalence of DAT in population control group. The results indicate the need for a larger sample of individuals to prove the role of hepatitis C virus alone as a trigger for DAT.
Acesso aberto (Open Access)
Distribution of hepatitis C virus genotypes among different exposure categories in the State of Pará, Brazilian Amazon
(2011-02) SAWADA, Leila; PINHEIRO, Andréia Cristina Costa; LOCKS, Daiane; PIMENTA, Adriana do Socorro Coelho; REZENDE, Priscila Rocha de; CRESPO, Deborah Maia; CRESCENTE, Jose Angelo Barletta; LEMOS, José Alexandre Rodrigues de; OLIVEIRA FILHO, Aldemir Branco de
INTRODUCTION: Epidemiological studies concerning HCV genotypic distribution in the Brazilian Amazon are scarce. Thus, this study determined the patterns of distribution of HCV genotypes among different exposure categories in the State of Pará, Brazilian Amazon. METHODS: A cross-sectional study was conducted on 312 HCV-infected individuals belonging to different categories of exposure, who were attended at the HEMOPA, CENPREN and a private hemodialysis clinic in Belém. They were tested for HCV antibodies using an immunoenzymatic test, RNA-HCV, using real-time PCR and HCV genotyping through phylogenetic analysis of the 5' UTR. The population groups were epidemiologically characterized according to data collected in a brief interview or medical consultation. RESULTS: Genotype 1 predominated in all the different categories of HCV exposure. HCV genotypic distribution among blood donors comprised genotypes 1 (94%) and 3 (6%). All patients with chronic hematologic diseases had HCV genotype 1. The genotypic distribution in illicit-drug users comprised genotypes 1 (59.6%) and 3 (40.4%). In patients under hemodialysis, genotypes 1 (90.1%), 2 (3.3%), and 3 (6.6%) were detected. Finally, the frequency of genotypes 1 and 3 was significantly different between the groups: BD and DU, PUH and DU, PUH and PCHD and PCHD and DU. CONCLUSIONS: The genotypic frequency and distribution of HCV in different categories of exposure in the State of Pará showed a predominance of genotype 1, regardless of the possible risk of infection.
Acesso aberto (Open Access)
Evaluation of the therapeutic response of hepatitis C in coinfected patients (HIV/HCV): a study of cases from a hospital for chronic liver diseases in the Eastern Brazilian Amazon
(2013-02) AMARAL, Ivanete do Socorro Abraçado; MÓIA, Lizomar de Jesus Maués Pereira; BARBOSA, Maria Silvia de Brito; DEMACHKI, Samia; ARAÚJO, Marialva Tereza Ferreira de; SOARES, Manoel do Carmo Pereira
INTRODUCTION: The aim of this study was to evaluate the therapeutic response of hepatitis C in patients coinfected with human immunodeficiency virus (HIV-1). METHODS: A retrospective study of 20 patients coinfected with HIV-1/HCV who were treated in the outpatient liver clinic at the Sacred House of Mercy Foundation Hospital of Pará (Fundação Santa Casa de Misericórdia do Pará - FSCMPA) from April 2004 to June 2009. Patients were treated with 180µg PEG interferon-α2a in combination with ribavirin (1,000 to 1,250mg/day) for 48 weeks. The end point was the sustained virological response (SVR) rate (HCV RNA negative 24 weeks after completing treatment). RESULTS: The mean age of the patients was 40±9.5 years, of which 89% (n=17) were male, and the HCV genotypes were genotype 1 (55%, n=11/20), genotype 2 (10%, n=2/20) and genotype 3 (35%, n=7/20). The mean CD4+ lymphocyte count was 507.8, and the liver fibrosis stages were (METAVIR) F1 (25%), F2 (55%), F3 (10%) and F4 (10%). The early virological response (EVR) was 60%, the end-of-treatment virological response (EOTVR) was 45% and the SVR was 45%. CONCLUSIONS: The median HCV viral load was high, and in 85% of cases in which highly active antiretroviral therapy (HAART) was used, none of the patients with F3-F4 fibrosis responded to treatment. Of the twenty patients treated, 45% achieved SVR and 45% achieved EOTVR. Studies that include cases from a wider region are needed to better evaluate these findings.
Acesso aberto (Open Access)
Hepatite C: prevalência e fatores de risco entre portadores do VIH/SIDA em Belém, Pará, na Amazônia brasileira
(Universidade Federal do Pará, 2004) MONTEIRO, Maria Rita de Cássia Costa; NASCIMENTO, Margarida Maria Passeri do; PASSOS, Afonso Dinis Costa; FIGUEIREDO, José Fernando de Castro
The objective of this investigation was to study the prevalence of hepatitis C virus infection and to identify possible risk factors for its transmission, in 406 adult patients with HIV/Aids who attended at public health services, in Belém city, Pará, Brazil. The anti-HCV was performed by third generation immunoenzymatic technique, and the HCV RNA by polymerase chain reaction. The overall prevalence of hepatitis C virus was 16% (CI: 12.4 - 19.6). Multivariate analysis showed association between virus C infection and age, with significant risk in the group about fifty years old or more (OR=9.75), blood transfusion (OR=4.74) and use of injecting drugs (OR=149.28). The hepatitis C virus infection was detected in 83.7% of intravenous drug users and 22.1% of transfused patients. These data indicate the efficient transmission of the virus through the percutaneous exposition and reaffirm the high risk to hepatitis C among injectable illicit drug user.
Acesso aberto (Open Access)
Hepatitis B and C virus infection among Brazilian Amazon riparians
(2011-10) OLIVEIRA, Claudia Suellen Ferro de; SILVA, Adenielson Vilar e; SANTOS, Kemper Nunes dos; FECURY, Amanda Alves; ALMEIDA, Marcella Kelly Costa de; FERNANDES, Adriana Prado; COSTA, Carlos Araújo da; FREITAS, Andrei Silva; CORVELO, Tereza Cristina de Oliveira; MARTINS, Luisa Caricio
INTRODUCTION: Viral hepatitis is a major public health concern in Brazil. There are few past studies on this issue, especially among riparian communities. This study aims at determining the seroprevalence of viral hepatitis B and C in the riparian community of Pacuí Island, within the Cametá municipality of Pará State, Brazil. Moreover, this study aims to investigate the principal risk factors that this community is exposed to. METHODS: The current study has accessed blood samples from 181 volunteers who have answered an epidemiological questionnaire. Analyses on serological markers have been tested with commercial ELISA kits for detecting HBsAg, total anti-HBc, anti-HBs, and anti-HCV. Within seroreactive patients for HCV, RT-PCR and line probe assay have been performed to identify the viral genotype. RESULTS: In the serological marker analysis for hepatitis B, no reactivity for HBsAg, rate of 1.1% for total anti-HBc, and rate of 19.3% for anti-HBs have been observed. On hepatitis C, 8.8% seroprevalence has been found, in which 62.5% have gotten viral RNA. Among the risk factors studied, the following have been highlighted: non-use of condoms, sharing of cutting instruments, use of illicit drugs, and reports of family disease with HBV or HCV. CONCLUSIONS: The vaccination coverage against HBV is low, and the high prevalence of HCV within this community has been observed.
Acesso aberto (Open Access)
Infecções pelos vírus das hepatites B e C e o carcinoma hepatocelular na Amazônia oriental
(2004) MIRANDA, Esther Castello Branco Mello; MÓIA, Lizomar de Jesus Maués Pereira; AMARAL, Ivanete do Socorro Abraçado; BARBOSA, Maria Silvia de Brito; CONDE, Simone Regina Souza da Silva; ARAÚJO, Marialva Tereza Ferreira de; CRUZ, Ermelinda do Rosário Moutinho da; DEMACHKI, Samia; BENSABATH, Gilberta; SOARES, Manoel do Carmo Pereira
In order to contribute to a better understanding of the possible role of hepatits B and C in the etiopathogenis of HCC in the East Amazon, there were studied 36 patients in Belém/PA. Serological hepatitis markers were evaluated and polymerase chain reaction assays were used to detect HBV-DNA and HCV-RNA. Alcohol abuse was observed in 33.3% and cirrhosis in 83.3%. In 88.9% of the sample, one or more hepatitis B markers were positive. Also, 8.3% those patients had anti-HCV simultaneously positive. The HBsAg serological test was positive in 58.3%; anti-HBc in 86%; anti-HBe in 85.7%; anti-HBe in 9.5%; IgM anti-HBc in 57.1%. The HBV DNA was found in 37.7% and in 65% of the HBsAg positive. The HCV RNA was detected in 8.5% and in 100% of' the patients positive to anti-HCV. The AFP was above the normal value in 88.9% of patients, with levels up to 400ng/ml in 75% of them. In conclusion, hepatitis B virus infection seems to be important in the etiology of HCC and improving measures such immunization and screening in the risk population should be emphasyzed.
Acesso aberto (Open Access)
A influência dos polimorfismos nos genes interferons lambda 3 lambda 4 e ancestralidade genética na infecção crônica pelo vírus da hepatite c e na resposta ao tratamento em uma população miscigenada de Belém-Pará-Brasil
(Universidade Federal do Pará, 2015) AMARAL, Ivanete do Socorro Abraçado; SANTOS, Eduardo José Melo dos; http://lattes.cnpq.br/6647221517242357; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
Chronic infection by Hepatitis C virus is a global public health problem in which more than half of infected people evolves to chronicity and can progress to cirrhosis with its complications as well as hepatocellular carcinoma. This study aimed to describe the polymorphisms in genes IL-28B and IFNL4 in a population of Belém with chronic infection by hepatitis C because they can influence the therapeutic response and the natural history of this infection. Three hundred and sixty-six patients were attended at Santa Casa de Misericórdia do Pará Hospital, confirmed by RT-PCR and 243 controls. The SNPs studied were determined by PCR Real Time technique and ancestry was assessed using 48 INDEL markers validated for European, African and Indian parental populations. Contributions of African, European and Amerindian ancestry were found in patients and controls, respectively (0.06 vs 0.04), (0,58 vs 0.662) (0.275) vs (0.214). The frequencies of rs-860 C/C, C/T, T/T (C/T and T/T) in patients and controls were respectively: (21% vs 38%), 59% vs 48%), (19% vs 14%), (79% vs 62%). The rs-917 genotype frequencies in patients and controls showed no significance and genotype frequencies in ss-590 were similar to those in rs-860. As predictors of therapeutic response: female gender, age below 45 years old, hepatic fibrosis stage (F1F2), rs-917 genotype T/T, HCV RNA <600.000UI/ml and platelets levels ≥ 150 x104 / mm3. Therapeutic response was associated with the rs-917 T/T and its mutant allele in heterozygous or homozygous was associated with therapeutic failure and F3 F4. In conclusion, from the point of view of genetics, one nitrogenous base exchange on a particular chromosome leads to large repercussions on different studied populations interfering with aspects related to the response to therapy, natural course of HCV infection. This information could lead to individualized treatment with better prospects of responses, increased vigilance for certain patients more likely to progress to more advanced stages of fibrosis and hepatocellular carcinoma.
Acesso aberto (Open Access)
Investigação de polimorfismo dos genes NFKB1, TYMS, UCP2 e SGSM3 em pacientes com hepatite C crônica em uma população da região norte do Brasil
(Universidade Federal do Pará, 2015-09-11) SOUZA , Susi dos Santos Barreto de; MOIA , Lizomar de Jesus Maués Pereira; http://lattes.cnpq.br/8335502787825672; DEMACHKI, Samia; http://lattes.cnpq.br/7568391537270652
The hepatitis C virus (HCV) affects about 130-150 million people worldwide. Sex, age, smoking, ethnicity, ancestry, and genetic polymorphisms may interfere with the progression of hepatitis C. We investigated the role of functional polymorphisms in genes NFKB1 (rs28362491), TYMS (rs16430), UCP2 and SGSM3 (rs56228771) with the unfavorable evolution of patients with chronic hepatitis C in a population in the northern region of Brazil. Epidemiological and clinical questionnaires were used to conduct a cross-sectional, observational and descriptive study to investigate polymorphisms. The relationship of these patients with the unfavorable evolution of 75 patients with chronic hepatitis C, in 2 groups (with and without cirrhosis), who underwent outpatient follow-up at two hospitals in Belém-PA, were identified. A panel of 48 Ancestral Information Markers (MIAs) was used as a method of genomic control in the study. It was revealed that the sex, age, smoking, alcoholism and polymorphisms of the TYMS and NFKB1 genes do not present statistical significance, respectively: p = 0.775; p = 0.070; p = 0.404; p = 0.498; p = 0.565 and p = 0.809. However, the polymorphisms of UCP2 and SGSM3 genes and African ancestry presented statistical significance. The 10% increase in African ancestry led to a reduction of 0.571 in the chance of developing cirrhosis of the liver, thus conferring a protective effect (P = 0.0417, OR = 0.429, CI = 95% = 0.170-0.898). The genotype of the polymorphism of the UCP2 gene was associated with a risk reduction (P = 0.05, OR = 0.0003, 95% CI = 0-1.90) and the genotype of the gene polymorphism SGSM3 was associated with significant risk (P = 0.024, OR = 7.106, 95% CI = 1,295-39,007) for developing cirrhosis of the liver. It is concluded that the African ancestry and the polymorphisms of the UCP2 and SGSM3 genes are related to the unfavorable evolution of patients with chronic hepatitis C.
Acesso aberto (Open Access)
Likely transmission of hepatitis C virus through sharing of cutting and perforating instruments in blood donors in the State of Pará, Northern Brazil
(2010-04) OLIVEIRA FILHO, Aldemir Branco de; PIMENTA, Adriana do Socorro Coelho; ROJAS, Márcia de Fátima Maciel de; CHAGAS, Márcia Cristina Munhoz; CRESPO, Deborah Maia; CRESCENTE, Jose Angelo Barletta; LEMOS, José Alexandre Rodrigues de
We determined the risk factors for HCV infection in blood donors in the State of Pará, Northern Brazil. We examined 256 blood donors seen at the Blood Bank of Pará State between 2004 and 2006. They were divided into two groups, depending on whether they were infected with HCV or not; 116 donors were infected with HCV, while the other 140 were free of infection. The HCV-RNA was detected by real-time PCR. All of the participants filled out a questionnaire about possible risk factors. The data were evaluated using simple and multiple logistic regressions. The main risk factors for HCV were found to be use of needles and syringes sterilized at home (OR = 4.55), invasive dental treatment (OR = 3.08), shared use of razors at home (OR = 1.99), sharing of disposable razors in barbershops, beauty salons, etc. (OR = 2.34), and sharing manicure and pedicure material (OR = 3.45). Local and regional health authorities should educate the public about sharing perforating and cutting materials at home, in barber/beauty shops, and in dental clinics as risk factors for HCV infection.
Acesso aberto (Open Access)
Marcadores ultra-sonográficos hepáticos em portadores assintomáticos do vírus da hepatite C, pré-doadores de sangue da Fundação HEMOPA, Belém-Pará
(Universidade Federal do Pará, 2003-12-29) MAGNO, José Emilio Campos; MONTEIRO, Maria Rita de Cassia Costa; http://lattes.cnpq.br/5536136455627983
ln order to evaluate the importance of the ultra-sonography, as an image diagnosis rnethod, in the detection of hepatics alterations in people who are symptomatics carrier of the hepatitis C vírus (HCV), this assay has studied people that are blood pre-donors of Fundação Hemopa, official reference hemocentre of Pará State, Brazil, between October and December of 2003,with the more important aims: to depict the hepatics markers of morphologics alterations; to find the frequency of thats alterations and to define the presence of association enter that’s morphologics alterations and the HCV infection. The assay has compared a central group - composed by 30 people HCV positive - and a control group - composed by 38 people HCV negative. Both of the research's groups were asymptomatic male and female with correct age to be blood donors. The donation selection have been done by the hemocentre, using their rotine proceeding. The paper has proceeded using pared people by sex and age. The groups have shown young age as most of all, wich is a normal hemocentre profile. Hepatomegaly, hiperecogenicity of the parenchyma have been the most found alteration in both research's groups, however without significative statistic difference. The increasing of the liver cell fatness is the way we can explain these alterations, reminding that the inflammation and the fibrotic components can increase this hiperecogenicity of the tiver parenquime. Heterogenicity, hipoecogenics focus, visibility alterations of some inside tiver veins and splenomegaly have been some other topics without significative statistic difference between the central and control groups. Significative statistic difference were found in the liver superfície marker and in the c1assification by pontuation, exactly as Lin's scores, about liver superficies, parenchyma, inside veins and spleen index. The total points has classified people from central and control groups in thin standard - 80% in central group and 100% in control group -; heterogenics - 13,3% in central group and 0% in control group -; and nodular - 6,7 % in central group and 0% in control group. There weren't found in both groups nodes, mass, ascitis, increasing of the portal and spleen veins and collateral circulation. The young age of blood donors people could have minimized the ecographics markers found, because of the chronics evolution tendence, slow and silent, of the VHC infection, with lately expressions of chronics hepatitis's morphologics alterations, cirrhosis and hepatocellular carcinoma.
Acesso aberto (Open Access)
Pesquisa e genotipagem do vírus da hepatite C em portadores de doenças renais crônicas submetidos à hemodiálise
(Universidade Federal do Pará, 2012-12-20) FREITAS, Maria de Jesus Rodrigues; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769
Patients with chronic kidney disease are at high risk of acquiring hepatitis C virus (HCV). The prevalence of hepatitis C in hemodialysis units is high. The study aimed to assess the presence of HCV and its different genotypes in patients with chronic kidney disease who underwent hemodialysis in Belém and metropolitan area in the state of Pará, Brazil, determining the prevalence of the virus genotypes and epidemiological characteristics of patients with chronic kidney disease. We conducted cross-sectional study, hemodialysis units in seven cities of Belém and metropolitan area in the period from October 2011 to April 2012. A questionnaire was applied to social data, epidemiological and on the presence of risk factors for viral hepatitis. Biological material was collected from the patients for HCV PCR and ELISA tests. Patients with the presence of viral RNA were evaluated for genotypes. The prevalence of antibodies to HCV between the groups was 8.4%, while 5.4% had viral RNA, with 0.1% among those who were negative. Genotype 1 was the most prevalent, with 86.1%, followed by type 2, with 11.6%. The type 3 was only 2.3%. Whereas epidemiological analysis showed male predominance, age 49, married or in a stable, low education level and family income of up to two minimum wages. The main cause of CKD was diabetes mellitus (34.4%), followed by glomerulonephritis (18.6%) and hypertension (17.1%). The duration of hemodialysis was significantly important risk factor for acquiring HCV (p = 0.012), with the majority of HCV patients who acquired the disease during hemodialysis were above 5 years of treatment (p = 0.0001). Another risk factor associated with HCV was prior organ transplant. We conclude that, in Belém and the metropolitan area, the prevalence of HCV in hemodialysis was high and the most frequent genotype is the same as the general population in northern Brazil.
Acesso aberto (Open Access)
Prevalência de Hepatite C em pacientes em terapia de substituição renal na cidade de Imperatriz – Maranhão
(Universidade Federal do Pará, 2012) SILVA, Maria Izabel Leite da; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769
Hepatitis C is considered a public health problem in Brazil and worldwide, with a high risk of becoming chronic, and complications such as hepatocellular carcinoma and liver cirrhosis. Patients on hemodialysis have a high risk for infection with hepatitis C for many reasons. Studies show high prevalence rates in dialysis units worldwide. In the state of Maranhao there´s no studies showing the profile of HCV in the population of patients with chronic renal failure. The main objective of this study is to evaluate the prevalence of hepatitis C in patients undergoing renal replacement therapy in the city of Imperatriz-Maranhao. As well as to investigate the main risk factors involved in transmission of HCV in this population. This is a cross-sectional study conducted at a clinic of kidney diseases, the period between January and December 2010. The target population for the study consisted of 181 patients on renal replacement therapy, hemodialysis or peritoneal dialysis. We used a questionnaire designed to obtain epidemiological data was collected peripheral blood sample, in order to perform the search for anti-HCV testing and molecular biology research and genotyping of viral RNA. The results showed a prevalence of 7.2% (13/181) in HCV patients. A higher frequency of males among patients with HCV (92%). The risk factor that stood out was the duration of hemodialysis, where 30.8% of patients over 15 years of treatment had antibodies against HCV and had abnormal ALT levels. The prevalence of Hepatitis C was high in the study unit. The duration of dialysis was considered crucial for the positivity of the virus, males had higher incidence. Individuals with chronic HCV infection had ALT levels higher than those without chronic hepatitis C infection.