Navegando por Assunto "Histonas"

Agora exibindo 1 - 2 de 2

Acesso aberto (Open Access)
Efeitos do exercício voluntario na recuperação de hemissecção da medula espinhal: mudanças na rede perineuronal e acetilação de histonas
(Universidade Federal do Pará, 2014-03-31) SFAIR, Márcio José Teixeira; BAHIA, Carlomagno Pacheco; http://lattes.cnpq.br/0910507988777644; PEREIRA JÚNIOR, Antônio
Regular physical exercise prevents and combats various diseases over time, especially as a great therapeutic tool for the treatment of lesions in the central nervous system (CNS). After transection (complete or incomplete / hemisection) spinal cord reactive glial cells secrete substances inhibitory to axonal regeneration , for example , molecules of chondroitin sulfate proteoglycans (PGSCs) that play an important role in the formation of a physical barrier chemical , called glial scar, which prevents the growth of axons damaged by injury. Research involving experimental model of spinal cord injury and rehabilitation by exercise have obtained promising results. However, the physiological and molecular mechanisms by which promote these positive results are still poorly understood. The objective of this study was to analyze the recovery of motor function of the hind paw after voluntary exercise protocol in an experimental model of spinal cord hemisection and investigate two molecular mechanisms involved in functional recovery: the degradation of the PGSCs Perineuronal networks and histone acetylation. To do this, twenty-four (24) male Wistar rats (Rattus norvegicus) mice were used and divided into 3 groups (control, trained and untrained). Except for the control group, all animals were accustomed to racing wheels and followed underwent an experimental surgery hemisection of the spinal cord at the time of the 8th thoracic vertebra. Our results showed that voluntary exercise on wheels racing after experimental spinal cord injury promoted recovery of motor function of the affected hind paw, but did not observe qualitative differences in histone acetylation and degradation PGSCs between groups.
Acesso aberto (Open Access)
Estudo teórico da reação de metilação da proteína lisina metiltransferase (pkmt)
(Universidade Federal do Pará, 2019-10-09) GOMES, Guelber Cardoso; LIMA, Anderson Henrique Lima e; http://lattes.cnpq.br/2589872959709848; https://orcid.org/0000-0002-8451-9912; SILVA, Jerônimo Lameira; http://lattes.cnpq.br/7711489635465954; https://orcid.org/0000-0001-7270-1517
Cancer is one of the main targets in academic research and its understanding is related to gene regulation through histone methylation. G9a protein is responsible for the methylation of histone 3 Lysine 9 (H3K9), which can perform one or two methylations on this specific residue. Thus, computational techniques were used to describe this reaction through QM/MM simulation using the techniques of SEP, PMF in the Dynamo program and the mechanism in the AMBER program with the methods AM1, AM1D, PM3, PM6 and RM1 determining the best method. the parameters to describe the reaction. The results show that the reaction for deprotonation of Lysine 9 showed better values in the Amber18 program, being close to expected through a direct transfer of Lysine 9 to Tyrosine 1154 with the RM1 method and energy barrier of 27.15 kJ/mol. The transfer of the methyl group from the SAM molecule to Lysine 9 showed that the PM6 method using the Dynamo PMF technique had an energy barrier of 72.80 kJ/mol which is close to that obtained by the experimental data.