Navegando por Assunto "Imunomodulador"

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Acesso aberto (Open Access)
Ação do metabólito secundário 5-hidroxi-2-hidroximetil gama-pirona isolado de fungos do gênero Aspergillus sobre monócitos humanos in vitro
(Universidade Federal do Pará, 2012-06-15) COSTA, Josineide Pantoja da; SILVA, Edilene Oliveira da; http://lattes.cnpq.br/7410116802190343
The 5-hydroxy-2- hydroxymethyl-gamma-pyrone (HMP) is a secondary metabolite synthe-sized by some species of fungi from Aspergillus, Penicillium and Acetobacter genera. The HMP has several applications, being used as antioxidant, tyrosinase inhibitor, protective agent against radiation and antitumor. Recently, it was also shown that this metabolite acts as a macrophage activator. However, the effect of HMP in human monocytes is unknown. Thus, the aim of this study was to evaluate the effects of HMP on the cell viability and differentia-tion of human blood monocytes in vitro. Human peripheral leucocytes were obtained from blood bag donated from Fundation Hemocenter of Para State. Cell isolation was performed using HISTOPAQUE® 1077-density-gradient. Monocytes were treated for 24, 48 and 72 hours with 50 and 100 μg/mL of HMP. The ultrastructural analysis of treated monocytes showed spreading ability, high number of cytoplasmatic projections and vacuoles, features that are often observed in activating cells. Immunofluorescence analysis of the expression of surface protein specific for the macrophage (F4/80), demonstrated that human monocytes treated with 50 and 100 μg/mL for 48 and 72 h showed the similar pattern of expression of proteins to that of human monocytes differentiated by macrophage colony-stimulating factor (M-CFS). The viability test used showed that HMP has no citotoxicity effect on human mon-ocytes when treated with 50 and 100 μg/mL of HMP. These results demonstrate a new role for HMP as an immunomodulator agent, inducing the differentiation of monocytes into macrophages.
Acesso aberto (Open Access)
Atividade antimicrobiana, antioxidante e imunomoduladora de Agaricus brasiliensis e Ilex paraguariensis in vitro e em modelo de sepse murino
(Universidade Federal do Pará, 2016-09-15) NAVEGANTES, Kely Campos; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390
Sepsis is an organ dysfunction caused by a dysregulated immune response to an infection, the initial therapeutic approach to sepsis are broad spectrum antimicrobials, which is not sufficient for control of infection, requiring association with other therapies. Thus, Ilex paraguariensis where to be a potent antimicrobial, antioxidant and Agaricus brasiliensis has immunomodulatory properties could be a new source of therapy. Thus, this study aimed to evaluate the antimicrobial effect, antioxidant and immunomodulatory in vitro and in vivo of the extracts. Therefore, in this study, we evaluated the in vitro antimicrobial activity of aqueous extracts from A.brasiliensis and I. paraguariensis against Staphylococcus aureus and Escherichia coli by the method of microdilution and by spectophotometry for determination of minimum inhibitory concentration and cultivation technique on petri dish for minimum bactericidal concentration, cytotoxicity in macrophages was evaluated, nitric oxide (NO) production, proliferation, phagocytosis, equivalent antioxidant capacity to Trolox (TEAC) and determination of the total antioxidant activity by capturing the free radical and reactive species production oxygen (ROS). In the swiss mice with induced sepsis were pretreated with aqueous extracts of A.brasiliensis and I. paraguariensis, after 12 and 24 hours collected their samples and evaluated the survival, leukocyte migration, hemogram, bacterial load, NO production, malondialdehyde (MDA), TEAC and ex vivo we evaluated phagocytic capacity and release of ROS. The A.brasiliensis showed no antimicrobial activity in vitro, remained viable cells reduced the phagocytic capacity, increased NO, but in the presence of LPS reduced, showed proliferative effect, but in the presence of mitogen had antiproliferative effect and has a strong antioxidant activity and capacity to sequester radicals in vitro. I.paraguariensis presented antimicrobial activity as well as cytotoxic effect induced phagocytic capacity increased NO, but in the presence of LPS reduced, had proliferative effect, antioxidant activity and capacity to sequester radicals in vitro. In vivo model of sepsis, both increased the survival of animals, A.brasiliensis reduced leukocyte influx while Ilex increased, only A.brasiliensis had hemogram similar to sham, both extracts reduced bacterial load and levels decreased NO, MDA and increased antioxidant levels in the tissues, in addition, both reduced the production of both extracts present ERO. Although excellent in vitro results, the aqueous extract from A.brasiliensis was found to be most promising as an adjuvant therapy in sepsis that I. paraguariensis due to its high antimicrobial activity, antioxidant and anti-inflammatory in vivo.