Navegando por Assunto "Indometacina"

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Acesso aberto (Open Access)
Avaliação da influência do tratamento com indometacina no aprendizado e na memória espacial em modelo murino de diabetes tipo 1
(Universidade Federal do Pará, 2017-05-25) SANTOS, Gabriel Cardoso de Queiroz; BASTOS, Gilmara de Nazareth Tavares; http://lattes.cnpq.br/2487879058181806
Diabetes mellitus (DM) is the group of metabolic disorders that has as a common characteristic the disregulation of blood glucose levels, invariably leading to hyperglycemia. This disease has become the most frequent in the adult population, mainly in developing countries, causing several serious consequences such as cardiovascular and renal diseases, factors responsible for a high mortality rate of the individuals affected. In addition that consequences, which are better investigated and described in the literature, other types of complications are observed. Clinical and experimental studies demonstrate that both type 1 and type 2 diabetes mellitus may contribute to the development of cognitive deficits and dementias. However, the mechanisms that lead to such disorders are not yet fully understood. A study using the non-selective non-steroidal anti-inflammatory, indomethacin, has shown that aspects related to impaired neuronal plasticity in diabetes can be reversed, demonstrating that these disorders may be modulated by neuroinflammatory changes. The aim of the present study was to evaluate the influence of chronic treatment with indomethacin on memory and learning in a murine model of type 1 diabetes mellitus (T1DM). Using the open field test, Y-maze test and Morris water maze test we investigated the indomethacin effects on behaviors changes after aloxan inducing T1DM. Indomethacin significantly decrease related behaviors to the anxious state in Open field test. This treatment also reversed space work memory deficits in the Y-maze test, and learning and spatial memory deficits in the Morris Water Maze. Thus, it can be concluded that chronic treatment with indomethacin has beneficial effects on the cognition of mice submitted to type 1 diabetes mellitus.
Acesso aberto (Open Access)
A indução do comportamento tipo ansiedade e estresse oxidativo pela indometacina no cérebro do Danio rerio (Zebrafish) é prevenida pelo alfa-tocoferol
(Universidade Federal do Pará, 2021-01) PINHEIRO, Jéssica Souza; OLIVEIRA, Karen Renata Herculano Matos; http://lattes.cnpq.br/3032008039259369; SILVA, Anderson Manoel Herculano Oliveira da; http://lattes.cnpq.br/8407177208423247
The non-steroidal anti-inflammatory drugs are among the most used and prescribed in the world, however this type of drug has several side effects at the neural level. Studies related to neurobehavioral and neurochemical damage of this class of drug are still necessary for a better understanding of all the possible damages that they can cause. As a result, indomethacin, which is an NSAID, has been widely used to treat pathologies such as rheumatoid arthritis, musculoskeletal injuries, osteoarthritis and postoperative pain. Indomethacin non-selectively blocks the enzymes COX-1 and COX-2, acting to decrease the production of prostaglandins. Therefore, this study proposed that the indomethacin could be generated anxiogenic effects and oxidative stress in the brain, and whether the antioxidant α-tocopherol exercised protection against the possible damage caused by indomethacin in zebrafish. The animals used were fish of the species Danio rerio (n=160), subdivided into the following groups: Control - Saline 0.9%; Indomethacin - INDO 0.5 mg/kg; INDO 0.75 mg/kg; INDO 1.0 mg/kg; INDO 2.0 mg/kg; INDO 3.0 mg/kg; α-Tocopherol - TF; TF + INDO 1 mg/kg; TF + INDO 2 mg/kg and were subjected novel tank diving test, the parameters time on top, freezing, erratic swimming and crossed quadrants were analyzed. The statistical analysis of the results was performed using one-way ANOVA with a post-test bonferroni or tukey for comparison between groups, with values with p <0.05 being considered significant. The results regarding the behavioral parameters and oxidative stress were expressed as mean ± standard error or standard deviation. The parameters that showed statistical differences were the time at the top and freezing, where the animals of the groups INDO 0.5 mg/kg, INDO 0.75 mg/kg, INDO 1 mg/kg and INDO 2 mg/kg explored for less the top of the apparatus compared to the CTRL group. In the freezing parameter the groups treated with indomethacin INDO 0.5 mg/kg, 0.75 mg/kg and 2 mg/kg did not show statistical differences with the CTRL group, however there was a difference between the CTRL and INDO groups 1 mg/kg. In the freezing parameter, the animals in the INDO 1 mg/kg group showed a longer time without movement compared to the CTRL group. In the other parameters, there were no significant differences between the groups treated with the control group. The analysis of lipid peroxidation, the INDO 1 mg/kg and INDO 2 mg/kg groups showed an increase in MDA production compared to the CTRL group, thus inferring that there was an increase in oxidative stress when animals were treated with indomethacin. The α-tocopherol exercised protection when animals were previously treated in both the TF + INDO 1 mg/kg group and the TF + INDO 2 mg/kg group compared to the INDO 1 mg/kg and INDO 2 mg/kg groups, respectively. Therefore, indomethacin is involved in inducing anxiety-like behavior and oxidative stress in zebrafish brains.
Acesso aberto (Open Access)
Neurogênese endógena induzida por acidente vascular encefálico experimental após inibição da ativação microglial/macrofágica com o anti-inflamatório indometacina
(Universidade Federal do Pará, 2011-05-16) LOPES, Rosana Telma Santos; LEAL, Walace Gomes; http://lattes.cnpq.br/2085871005197072
Stroke results from the transitory or permanent reduction of cerebral blood flow. It can be classified as hemorrhagic or ischemic. Ischemic stroke is responsible for around 87% of all cases. This acute neural disorder is the second cause of mortality and disability around the world and the main cause of death in Brazil. It has been shown that neuroblasts migrate to the ischemic striatum following middle cerebral artery occlusion (MCAO) and partially replace neurons lost during ischemia. Nevertheless, most of the migrating neuroblasts die in the first weeks following MCAO and inflammatory events, mainly microglia activation, may underlie neuroblast death. In this study, we investigated the effects of the nonsteroidal anti-inflammatory indomethacin on microglial activation, neuronal preservation and adult neurogenesis following experimental MCAO in adult rats. Animals were submitted to endothelin-1 induced- MCAO and treated (i.p) with indomethacin (N=8) or sterile saline (N=8) for 7 days and perfused at 8 or 14 days. Immunohistochemistry was performed to assess neuronal loss (anti-NeuN), microglial activation (anti-Iba1 and ED1) and migrating neuroblasts (anti-DCX). The numbers of NeuN, ED1 and DCX positive cells per field were counted in the ischemic striatum or subventricular zone. Indomethacin treatment reduced microglial activation in general and the number of ED1+ cells at both 8 and 14 days (±6,9 and ±3,0 cells respectively) postinjury, compared to control (±7,9 or ±6,5 cells, p<0.001, ANOVA-Tukey). There was an increase in the number of DCX+ cells in both subventricular zone (SVZ) and striatum at the same survival times. There was no difference in the number of NeuN positive cells between groups in all investigated survival times. The results show that indomethacin treatment induces inhibition of microglial activation concomitant with increased neuroblast proliferation and migration following MCAO. This is a promising outcome, considering that indomethacin is already used in non-neural human diseases and that adult neurogenesis may underlie functional recovery following stroke.